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ABSTRACT Numerous observations suggest that chromosome instability is caused by mitotic abnormalities such as errors in the partitioning of chromosomes. _Chfr_ was recently defined as a

central component of a new mitotic checkpoint that delays chromosome condensation in response to mitotic stress. _Chfr_ was shown to be frequently inactivated in several human neoplasms,

including colon, lung and esophageal cancers. To test whether _Chfr_ inactivation may lead or participate to chromosomal instability (CIN), we analysed the genetic and epigenetic status of

the gene in a large panel of primary colon and breast cancers, as well as in colon and breast cancer cell lines displaying either a microsatellite instability or a CIN. Our results confirm

that _Chfr_ is frequently inactivated in colon cancers, through a mechanism of hypermethylation of the promoter sequences. In contrast, the loss of _Chfr_ expression appears to be a rare

event in breast cancers. Furthermore, our data demonstrate that _Chfr_ inactivation is not associated with CIN in these frequent types of human cancers. Access through your institution Buy

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OTHERS CHROMOSOMAL INSTABILITY AS A DRIVER OF CANCER PROGRESSION Article 29 July 2024 A PAN-CANCER COMPENDIUM OF CHROMOSOMAL INSTABILITY Article 15 June 2022 THE TWO SIDES OF CHROMOSOMAL

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Sen S . (1998). _Nat. Genet._, 20, 189–193. Download references ACKNOWLEDGEMENTS We thank T Halazonetis for _Chfr_ constructs. This work was supported by grants from the Comité Départemental

du Rhône and the Comité Départemental de l'Ain de la Ligue de Lutte contre le Cancer, and from the Association pour la Recherche sur le Cancer. AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * Unité d'Oncologie Moléculaire, INSERM U590, Centre Léon Bérard, 28 rue Laënnec, Lyon, Cedex 08, 69373, France Jacques Bertholon, Qing Wang, Nicole Falette, Carole Verny, 

Jessie Auclair, Christelle Chassot, Claudine Navarro & Alain Puisieux * Faculté de Pharmacie, 8 avenue Rockefeller, Lyon, Cedex 08, 69373, France Jacques Bertholon & Alain Puisieux *

Fédération de Spécialités Digestives, Hôpital Edouard Herriot, Place d'Arsonval, Lyon, Cedex 03, 69437, France Jean-Christophe Saurin Authors * Jacques Bertholon View author

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Jessie Auclair View author publications You can also search for this author inPubMed Google Scholar * Christelle Chassot View author publications You can also search for this author inPubMed

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Puisieux. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bertholon, J., Wang, Q., Falette, N. _et al._ _Chfr_ inactivation is not associated to

chromosomal instability in colon cancers. _Oncogene_ 22, 8956–8960 (2003). https://doi.org/10.1038/sj.onc.1207078 Download citation * Received: 20 May 2003 * Revised: 04 August 2003 *

Accepted: 05 August 2003 * Published: 04 December 2003 * Issue Date: 04 December 2003 * DOI: https://doi.org/10.1038/sj.onc.1207078 SHARE THIS ARTICLE Anyone you share the following link

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content-sharing initiative KEYWORDS * _Chfr_ * hypermethylation * chromosome instability * breast cancer * colon cancer