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ABSTRACT A CONSIDERABLE amount of microscope work involved in cytological studies of plant roots can be obviated by the preparation of a cell suspension from a number of roots ; the cell

suspension can then be examined instead of a large number of sections or squashes of individual roots. This is of particular advantage in studies in which the examination of a large cell

population is required for the evaluation of the cytological effects of a particular experimental treatment, such as X-radiation. A number of techniques have been described for making cell

suspensions from plant roots1–3, all of which either produce a large number of broken cells or require the use of enzymes. The following simplified method without the use of enzymes has

given satisfactory results with _Vicia faba_ root-tips, the number of cells broken being negligible. Access through your institution Buy or subscribe This is a preview of subscription

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ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS SUSPENSION CULTURE OF STEM CELLS

ESTABLISHED OF _CALENDULA OFFICINALIS_ L. Article Open access 03 January 2024 EXTRACELLULAR VESICULO-TUBULAR STRUCTURES ASSOCIATED WITH SUBERIN DEPOSITION IN PLANT CELL WALLS Article Open

access 18 March 2022 PRECISE TRACKING OF NANOPARTICLES IN PLANT ROOTS Article 05 September 2024 REFERENCES * Brown, R., _J. Exp. Bot._, 2, 96 (1951). Article  Google Scholar  * Harris, B.

J., and Blackman, G. E., _Nature_, 173, 642 (1954). Article  ADS  Google Scholar  * Setterfield, G., Schreiber, R., and Woodard, J., _Stain Technology_, 29, 113 (1954). Article  CAS  Google

Scholar  * Brown, R., and Richless, P., _Proc. Roy. Soc._, B, 136, 110 (1949). Article  ADS  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Medical

Research Council Experimental Radiopathology Research Unit, Hammersmith Hospital, London, W.12 SHIRLEY HORNSEY Authors * SHIRLEY HORNSEY View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE HORNSEY, S. Preparation of Cell Suspensions from Plant Roots for

Cytological Studies. _Nature_ 176, 744–745 (1955). https://doi.org/10.1038/176744a0 Download citation * Issue Date: 15 October 1955 * DOI: https://doi.org/10.1038/176744a0 SHARE THIS ARTICLE

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