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ABSTRACT The translocation t(11;18)(q21;q21), which is the most frequent chromosomal aberration in extranodal marginal zone B cell lymphomas of MALT-type, was characterised in a series of 34

biopsies, including 18 gastric non-Hodgkin's lymphomas (NHL) of MALT-type, six MALT-type NHL of extragastral origin and 10 extranodal large B cell lymphomas (LBL). Based on

fluorescence _in situ_ hybridisation, STS-PCR analysis and screening of genomic PAC libraries, a physical map of contiguous DNA probes on chromosome 11 was constructed containing the

anti-apoptotic genes _API2_ and _API1_ adjacent to the translocation breakpoint. RACE-PCR experiments revealed _MALT1_ the chromosome 18-derived fusion partner of _API2_, which has also been

reported recently by other groups. RT-PCR analysis and DNA sequencing demonstrated the expression of an _API2-MALT1_ fusion transcript in 18/24 gastral and extragastral MALT-type lymphomas.

In none of 10 LBLs was a translocation specific RT-PCR product detected. Five variants of the fusion transcript were identified and in all instances the open reading frame of the fused

portion of the _MALT1_ gene was maintained. The molecular analysis of these variants allowed the design of optimised assays for the diagnosis of the _API2-MALT1_ gene rearrangement. Access

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SIMILAR CONTENT BEING VIEWED BY OTHERS GENOMIC LANDSCAPE OF EPSTEIN–BARR VIRUS-POSITIVE EXTRANODAL MARGINAL ZONE LYMPHOMAS OF MUCOSA-ASSOCIATED LYMPHOID TISSUE Article 24 December 2021 LARGE

B-CELL LYMPHOMAS WITH _CCND1_ REARRANGEMENT HAVE DIFFERENT IMMUNOGLOBULIN GENE BREAKPOINTS AND GENOMIC PROFILE THAN MANTLE CELL LYMPHOMA Article Open access 23 September 2024 LONG-TERM

MONITORING OF GASTRIC MUCOSA-ASSOCIATED LYMPHOID TISSUE LYMPHOMA IN PATIENTS WITH EXTRA COPIES OF THE _MALT1_ GENE Article Open access 29 February 2024 REFERENCES * Isaacson P, Wright DH .

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at Roswell Park Cancer Institute by Panos A Ioannou (RPCI-1), Chira Chen and Baohui Zhao (RPCI-3), and Baohui Zhao (RPCI-4 and 5). High density filters and clones were kindly provided by the

Resource Center/Primary Database of the German Human Genome Project, Berlin, Germany. Michael James kindly provided information on the physical map of the YACs. The authors gratefully thank

Simone Schindelmann, Sandra Weitz and Ralf Klären for their technical assistance. Finally, we also acknowledge Peter Marynen for open discussion. This work was supported by grants from the

Deutsche Forschungsgemeinschaft (Ka 1449/1–1) and the Sander-Stiftung (94.025.2 and 98.086.1) AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * ‘Organisation komplexer Genome’, Deutsches

Krebsforschungszentrum, Heidelberg, Germany J Kalla, C Schaffner, S Wolf & P Lichter * Innere Medizin III, Universität Ulm, Ulm, Germany S Stilgenbauer & H Döhner * Pathologisches

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and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kalla, J., Stilgenbauer, S., Schaffner, C. _et al._ Heterogeneity of the _API2-MALT1_ gene rearrangement in MALT-type lymphoma.

_Leukemia_ 14, 1967–1974 (2000). https://doi.org/10.1038/sj.leu.2401918 Download citation * Received: 22 May 2000 * Accepted: 05 July 2000 * Published: 02 November 2000 * Issue Date: 01

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shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * MALT-type lymphoma * extranodal

large B cell lymphoma * t(11 * 18)(q21 * q21) * _API2_ * _MALT1_