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ABSTRACT Although the fixation of ligand to haemoglobin (Hb) is known to be accompanied by changes in protein conformation1 regulating the oxygen exchange in blood, the mechanism triggering

these changes remains undecided2–4. We now report a dynamic approach to this problem using results obtained in a nanosecond laser photolysis study of carboxyhaemoglobin (HbCO) and its

isolated subunits. The study is based on our previous observation5 of a structural evolution of free Hb after photodeligation, manifested through slight variations of the protein spectrum in

the microsecond range. It is now found that the isolated subunits also show this behaviour. The duration of the spectral evolution is ∼2_µ_s for the three proteins and the activation energy

of the process ∼9 kcal mol−1. The spectral evolution is attributed to local conformation changes at the haem region, occurring during the structural relaxation of the freshly deliganded

protein. The results for the isolated chains show that such changes exist even in the absence of the R-T transition. Access through your institution Buy or subscribe This is a preview of

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STUDY OF THE SPIN CROSS-OVER DYNAMICS IN HAEM PROTEINS Article Open access 18 August 2020 INFLUENCE OF PUMP LASER FLUENCE ON ULTRAFAST MYOGLOBIN STRUCTURAL DYNAMICS Article Open access 14

February 2024 ULTRAFAST COHERENT MOTION AND HELIX REARRANGEMENT OF HOMODIMERIC HEMOGLOBIN VISUALIZED WITH FEMTOSECOND X-RAY SOLUTION SCATTERING Article Open access 16 June 2021 REFERENCES *

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AUTHORS AND AFFILIATIONS * Laboratoire de Photophysique Moléculaire du CNRS, Université de Paris-Sud, Orsay Cédex, 91405, France L. Lindqvist, S. El Mohsni & F. Tfibel * Laboratoire de

Biologie Physico-Chimique, UER Biomédicale, 45 rue des Saints-Pères, 75270, Paris, Cédex 06, France S. El Mohsni & B. Alpert Authors * L. Lindqvist View author publications You can also

search for this author inPubMed Google Scholar * S. El Mohsni View author publications You can also search for this author inPubMed Google Scholar * F. Tfibel View author publications You

can also search for this author inPubMed Google Scholar * B. Alpert View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Lindqvist, L., El Mohsni, S., Tfibel, F. _et al._ Transient haem–globin interactions in photodeligated carboxyhaemoglobin and subunits.

_Nature_ 288, 729–730 (1980). https://doi.org/10.1038/288729a0 Download citation * Received: 14 July 1980 * Accepted: 04 November 1980 * Issue Date: 18 December 1980 * DOI:

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