Reader TTS

ABSTRACT The transplantation antigens H–2K, H–2D and H–2L are developmentally regulated1–3, highly polymorphic4 cell surface proteins encoded by the major histocompatibility gene complex

(MHC)5–7. First detectable on the early embryo2,3, they are subsequently expressed on most somatic cells of the adult mouse in association with the protein _β_2-microglobulin (_β_2M; ref.

5). Cultured F9 embryonal carcinoma (EC) cells can be induced to differentiate along alternative pathways to form either parietal8 or visceral9 extra-embryonic endoderm, each concomitant

with a change in morphology and pattern of gene expression. Previous reports have demonstrated an increased level of transplantation antigens in differentiated F9 EC cells10–14, but the cell

types expressing them were not defined. Here we show that the level of MHC H–2Kb and _β_2M transcripts is increased during both pathways of this differentiation. Expression of a foreign MHC

_H_–_2K_bm1 gene was found to be regulated in a similar manner when the gene was introduced into EC cells. In contrast, an introduced rabbit _β_-globin gene was not regulated but expressed

constitutively. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution

Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full

article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs *

Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS IMMUNE-PRIVILEGED TISSUES FORMED FROM IMMUNOLOGICALLY CLOAKED MOUSE EMBRYONIC STEM CELLS SURVIVE LONG TERM IN ALLOGENEIC HOSTS

Article Open access 23 November 2023 TGF-Β1 TRANSCRIPTIONALLY PROMOTES 90K EXPRESSION: POSSIBLE IMPLICATIONS FOR CANCER PROGRESSION Article Open access 22 April 2021 IN VITRO AND IN VIVO

FUNCTIONS OF T CELLS PRODUCED IN COMPLEMENTED THYMI OF CHIMERIC MICE GENERATED BY BLASTOCYST COMPLEMENTATION Article Open access 25 February 2022 REFERENCES * Strickland, S., Smith, K. K.

& Maratti, K. K. _Cell_ 21, 347–355 (1980). Article  CAS  Google Scholar  * Ostrand-Rosenberg, S. _Curr. Topics dev. Biol._ 14, 147–168 (1980). Article  CAS  Google Scholar  * Webb, C.

G. _et al._ _J. exp. Med._ 146, 923–932 (1977). Article  CAS  Google Scholar  * Klein, J. _Science_ 203, 516–521 (1977). Article  ADS  Google Scholar  * Ploegh, H. L., Orr, H. T. &

Strominger, J. L. _Cell_ 24, 287–299 (1981). Article  CAS  Google Scholar  * Hood, L., Steimmetz, M. & Goodenow, R. _Cell_ 28, 685–687 (1982). Article  CAS  Google Scholar  * Klein, J.

_Biology of the Mouse Major Histocompatibility Complex_ (Springer, Berlin, 1975). Book  Google Scholar  * Strickland, S. & Mahdavi, V. _Cell_ 15, 393–403 (1980). Article  Google Scholar

  * Hogan, B. L. & Taylor, A. _Nature_ 291, 235–237 (1981). Article  ADS  CAS  Google Scholar  * Nicolas, J. F. _et al._ _Ann. Microbiol_ _A_ 126, 3–22 (1975). CAS  Google Scholar  *

Croce, C. M. _et al._ _Proc. natn. Acad. Sci. U.S.A._ 78, 5754–5748 (1981). * Knowles, B. B. _et al._ _Nature_ 288, 615–617 (1980). Article  ADS  CAS  Google Scholar  * Jacob, F. _Immunol.

Dev._ 33, 3–32 (1977). CAS  Google Scholar  * Tanaha, K., Appella, E. & Jay, G. _Cell_ 35, 457–465 (1983). Article  Google Scholar  * Cooper, A. R., Taylor, A. & Hogan, B. L. M.

_Devl Biol._ 99, 510–516 (1983). Article  CAS  Google Scholar  * Kurkinen, M. _et al._ _Nucleic Acids Res._ 11, 6200–6209 (1983). Article  Google Scholar  * Fames, J. R. _et al._ _Proc.

natn. Acad. Sci. U.S.A._ 78, 2253–2257 (1981). Article  ADS  Google Scholar  * Sherman, M. I. & Miller, R. A. _Devl Biol._ 63, 27–34 (1978). Article  CAS  Google Scholar  * Benham, P.

J., Quintero, M. A. & Goodfellow, P. M. _EMBO. J._ 2, 1963–1968 (1983). Article  CAS  Google Scholar  * Artzt, K. & Jacob, F. _Transplantation_ 17, 632–625 (1975). * Forejt, J. _Cold

Spring Harb. Symp. quant. Biol._ 48, 541–552 (1983). Google Scholar  * Andrews, D. W. & Goodfellow, P. N. _Somat. Cell Genet._ 6, 271–284 (1980). Article  CAS  Google Scholar  *

Correani, A. & Croce, C. M. _J. cell. Physiol._ 105, 73–99 (1980). Article  CAS  Google Scholar  * Weiss, E. H. _et al._ _Nature_ 301, 671–674 (1983). Article  ADS  CAS  Google Scholar 

* Wigler, G. R. _et al._ _Cell_ 16, 777–785 (1979). Article  CAS  Google Scholar  * Queen, C. & Baltimore, D. _Cell_ 33, 741–748 (1983). Article  CAS  Google Scholar  * Gillies, S. D.

_et al._ _Cell_ 33, 719–728 (1983). Article  Google Scholar  * Banerji, J. _et al._ _Cell_ 33, 729–740 (1983). Article  CAS  Google Scholar  * Murphy, D. _et al._ _Cell_ 35, 865–871 (1983).

Article  CAS  Google Scholar  * Liesi, P. _et al._ _Nature_ 306, 265–267 (1983). Article  ADS  CAS  Google Scholar  * Grosveld, G. C. _et al._ _Nature_ 295, 120–126 (1982). Article  ADS  CAS

  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratory of Gene Structure and Expression, National Institute for Medical Research, The Ridgeway, Mill

Hill, London, NW7 1AA, UK Arnon Rosenthal, Stephanie Wright, Richard Flavell & Frank Grosveld * Department of Molecular Biology, The Hebrew University, Hadassah Medical School,

Jerusalem, Israel Arnon Rosenthal & Howard Cedar * Biogen Research Corporation, 14 Cambridge Center, Cambridge, Massachusetts, 02142, USA Richard Flavell Authors * Arnon Rosenthal View

author publications You can also search for this author inPubMed Google Scholar * Stephanie Wright View author publications You can also search for this author inPubMed Google Scholar *

Howard Cedar View author publications You can also search for this author inPubMed Google Scholar * Richard Flavell View author publications You can also search for this author inPubMed 

Google Scholar * Frank Grosveld View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE

THIS ARTICLE Rosenthal, A., Wright, S., Cedar, H. _et al._ Regulated expression of an introduced MHC _H_–_2K__bm1_ gene in murine embryonal carcinoma cells. _Nature_ 310, 415–418 (1984).

https://doi.org/10.1038/310415a0 Download citation * Received: 14 February 1984 * Accepted: 21 May 1984 * Issue Date: 02 August 1984 * DOI: https://doi.org/10.1038/310415a0 SHARE THIS

ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard

Provided by the Springer Nature SharedIt content-sharing initiative