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ABSTRACT The lactose repressor protein from _Escherichia coli_ binds sugars, primarily galactosides, which modulate its interactions with operator DNA and thereby affect synthesis of the

_lac_ metabolic enzymes1. The affinity of the repressor for operator DNA is decreased by binding inducer sugars and increased by binding anti-inducer sugars2. Based on regions of the primary

structure implicated by genetic methods to be involved in sugar binding3–5, amino acid sequence homology between L-arabinose-binding protein (ABP) and _lac_ repressor has recently been

reported6. The sugar-binding sites for these two proteins might be expected to have similar structural features, as both bind L-arabinose and D-galactose. The high resolution structure of

ABP reported in the accompanying article7 provides complete definition of amino acids in the sugar-binding site. By identification of homologous residues in the _lac_ repressor, we have now

predicted the structure of the portion of the repressor sugar-binding site which accommodates the galactosyl moiety. This prediction provides the first potential view of the

inducer/anti-inducer site in the repressor protein. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS

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institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS STRUCTURAL ANALYSIS AND INSIGHT INTO EFFECTOR BINDING OF THE NIACIN-RESPONSIVE

REPRESSOR NIAR FROM _BACILLUS HALODURANS_ Article Open access 03 December 2020 MECHANISM OF NANR GENE REPRESSION AND ALLOSTERIC INDUCTION OF BACTERIAL SIALIC ACID METABOLISM Article Open

access 31 March 2021 X-RAY CRYSTALLOGRAPHY REVEALS MOLECULAR RECOGNITION MECHANISM FOR SUGAR BINDING IN A MELIBIOSE TRANSPORTER MELB Article Open access 02 August 2021 REFERENCES * Miller,

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INFORMATION AUTHORS AND AFFILIATIONS * Department of Biochemistry, Rice University, Houston, Texas, 77251, USA Clarence F. Sams, Nand K. Vyas, Florante A. Quiocho & Kathleen Shive

Matthews Authors * Clarence F. Sams View author publications You can also search for this author inPubMed Google Scholar * Nand K. Vyas View author publications You can also search for this

author inPubMed Google Scholar * Florante A. Quiocho View author publications You can also search for this author inPubMed Google Scholar * Kathleen Shive Matthews View author publications

You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sams, C., Vyas, N., Quiocho, F. _et al._

Predicted structure of the sugar-binding site of the _lac_ repressor. _Nature_ 310, 429–430 (1984). https://doi.org/10.1038/310429a0 Download citation * Received: 09 January 1984 * Accepted:

10 May 1984 * Issue Date: 02 August 1984 * DOI: https://doi.org/10.1038/310429a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

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