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ABSTRACT Neurotensin is a 13-amino acid peptide that is widely distributed in central and peripheral tissues of various mammalian species1. In peripheral tissues, the highest concentration

of neurotensin-like immunoreactivity is found in the ileum, where it is present in endocrine-like cells and nerve fibres2–4. The longitudinal smooth muscle layer of the guinea pig ileum,

where neurotensin has both a direct relaxant and an indirect contractile action, has been used extensively as a biological assay system for neurotensin5. We report here that the majority of

specific 3H-neurotensin binding sites is present in the guinea pig ileum circular smooth muscle layer, which is known to be innervated by a large proportion of the ileal non-adrenergic

inhibitory nerves6. Neurotensin produces a dose-dependent, tetrodotoxin-resistant relaxation, whereas the relaxation produced by field stimulation of the inhibitory nerves is

frequency-dependent and tetrodotoxin-sensitive. The calcium-dependent potassium channel blocker apamin7,8 inhibits both the neurotensin- and nerve stimulation-induced muscle relaxation.

Incubation of the circular smooth muscle preparation with a neurotensin antiserum substantially inhibited the nerve stimulation-induced relaxation, indicating a direct relationship between

the effects of neurotensin and of nerve stimulation. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS

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AND 5-HT (SEROTONIN), INDEPENDENT TO THE RHO KINASE AND EXTRACELLULAR CALCIUM PATHWAYS Article Open access 23 October 2023 GIANT PYRAMIDAL NEURONS OF THE PRIMARY MOTOR CORTEX EXPRESS

VASOACTIVE INTESTINAL POLYPEPTIDE (VIP), A KNOWN MARKER OF CORTICAL INTERNEURONS Article Open access 11 September 2024 THYROID HORMONE ACTIVATED UPPER GASTROINTESTINAL MOTILITY WITHOUT

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Unit, Medical Research Council Centre, Medical School, Hills Road, Cambridge, CB2 2QH, UK Michel Goedert, John C. Hunter & Mary Ninkovic Authors * Michel Goedert View author publications

You can also search for this author inPubMed Google Scholar * John C. Hunter View author publications You can also search for this author inPubMed Google Scholar * Mary Ninkovic View author

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Ninkovic, M. Evidence for neurotensin as a non-adrenergic, non-cholinergic neurotransmitter in guinea pig ileum. _Nature_ 311, 59–62 (1984). https://doi.org/10.1038/311059a0 Download

citation * Received: 01 June 1984 * Accepted: 02 July 1984 * Issue Date: 06 September 1984 * DOI: https://doi.org/10.1038/311059a0 SHARE THIS ARTICLE Anyone you share the following link with

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