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ABSTRACT During development, neurones find and interconnect with their targets in a remarkably precise way. The unfolding of neuronal specificity involves a series of highly specific

recognition events which are likely to be coordinated by the spatial and temporal expression of many different surface molecules. At early stages of development, neuronal recognition occurs

most dramatically at the tips of growing axons, at growth cones and their filopodia1,2. Previous studies on the grasshopper embryo suggest that specific filopodial contacts lead to the

stereotyped patterns of selective axonal fasciculation; these results support the ‘labelled pathways’ hypothesis which predicts that the different neighbouring axon fascicles in the

embryonic neuropil within filopodial grasp are differentially labelled3–8. To uncover the molecular labels on fasciculating embryonic axons, we screened 2,000 monoclonal antibodies generated

against the embryonic neuroepithelium9. Here we describe three antibodies which reveal surface antigens whose temporal and spatial expression during embryogenesis correlate with the

predictions of the model. In particular, the Mes-2 antibody recognizes an antigen which is transiently expressed on the surface of only 4 out of ∼ 1,000 neurones in each metathoracic

hemisegment during a short period of embryogenesis. The growth cones of two of these neurones fasciculate in the periphery and innervate the same target. Moreover, they transiently express

the Mes-2 surface antigen while doing so. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access

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Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional

subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS SPATIOTEMPORAL EXPRESSION OF _IGLON_ FAMILY MEMBERS IN THE DEVELOPING MOUSE NERVOUS SYSTEM

Article Open access 01 October 2021 A DEVELOPMENTAL ATLAS OF SOMATOSENSORY DIVERSIFICATION AND MATURATION IN THE DORSAL ROOT GANGLIA BY SINGLE-CELL MASS CYTOMETRY Article 27 October 2022

HOMEOBOX GENES AND THE SPECIFICATION OF NEURONAL IDENTITY Article 26 August 2021 REFERENCES * Bray, D. in _Cell Behavior_ (eds Bellairs, R., Curtis, A. & Dunn, G.) 299–318 (Cambridge

University Press, 1982). Google Scholar  * Letourneau, P. C. in _Neuronal Development_ (ed Spitzer, N. C.) 213–259 (Plenum, 1982). Book  Google Scholar  * Goodman, C. S., Raper, J. A., Ho,

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M. J., Ho, R. K. & Goodman, C. S. _Devl Biol._ 94, 391–399 (1982). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of

Biological Sciences, Stanford University, Stanford, California, 94305, USA Kathryn J. Kotrla & Corey S. Goodman Authors * Kathryn J. Kotrla View author publications You can also search

for this author inPubMed Google Scholar * Corey S. Goodman View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kotrla, K., Goodman, C. Transient expression of a surface antigen on a small subset of neurones during embryonic development. _Nature_ 311,

151–153 (1984). https://doi.org/10.1038/311151a0 Download citation * Received: 19 March 1984 * Accepted: 26 June 1984 * Issue Date: 13 September 1984 * DOI: https://doi.org/10.1038/311151a0

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