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ABSTRACT Adhesion of platelets to the subendothelial matrix of an injured vessel wall is an essential step in triggering the formation of a haemostatic plug. Fibronectin, collagen and

laminin are three major components of the subendothelial matrix which support platelet adhesion1. Receptors for fibronectin and collagen have been identified on platelets and are included in

the integrin family2–11. Here we report that adhesion of platelets to laminin is inhibited by a rat monoclonal antibody against the integrin family member, VLA-6. This antibody does not

affect platelet adhesion to fibrinogen, fibronectin or to type I and III collagen. Binding to laminin does not require platelet activation and is not inhibited by fibronectin and laminin

cell-attachment peptides. Platelet adhesion to laminin is supported by Mn2+, Co2+and Mg2+, but not by Ca2+, Zn2+ and Cu2+. This cation preference is distinct from that characteristic for

other platelet-adhesive glycoproteins8,12,13. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access

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subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS STRUCTURAL MECHANISM OF LAMININ RECOGNITION BY INTEGRIN Article Open access 29 June 2021

EFFECTS OF BACTERIAL LIPOPOLYSACCHARIDES ON PLATELET FUNCTION: INHIBITION OF WEAK PLATELET ACTIVATION Article Open access 23 July 2020 INTEGRIN Β3 DIRECTLY INHIBITS THE GΑ13-P115RHOGEF

INTERACTION TO REGULATE G PROTEIN SIGNALING AND PLATELET EXOCYTOSIS Article Open access 16 August 2023 REFERENCES * III, C. R., Engvall, E. & Ruoslahti, E. _J. Cell Biol._ 99, 2138–2145

(1984). Google Scholar  * Gardner, J. M. & Hynes, R. O. _Cell_ 42, 439–448 (1985). Article  CAS  PubMed  Google Scholar  * Plow, E. F., McEver, R. P., Coller, B. S., Woods, V. L. Jr

& Marguerie, G. A. _Blood_ 66, 724–727 (1985). CAS  PubMed  Google Scholar  * Pytela, R., Pierschbacher, M. D., Ginsberg, M. H., Plow, E. F. & Ruoslahti, E. _Science_ 231, 1559–1562

(1986). Article  ADS  CAS  PubMed  Google Scholar  * Giancotti, F. P. _et al._ _Blood_ 69, 1535–1538 (1987). CAS  PubMed  Google Scholar  * Piotrowicz, R. S., Orchekowski, R. P., Nugent, D.

J., Yamada, K. Y. & Kunicki, T. J. _J. Cell Biol_ 106, 1359–1364 (1988). Article  CAS  PubMed  Google Scholar  * Nieuwenhuis, H. K., Akkerman, J. W. N., Houdijk, W. P. M. & Sixma, J.

J. _Nature_ 318, 470–472 (1985). Article  ADS  CAS  PubMed  Google Scholar  * Santoro, S. A. _Cell_ 46, 913–920 (1986). Article  CAS  PubMed  Google Scholar  * Santoro, S. A., Rajpara, S.

M., Staatz, W. D. & Woods, V. J. Jr _Biochem. biophys. Res. Commun._ 153, 217–223 (1988). Article  CAS  PubMed  Google Scholar  * Kunicki, T. J. _et al._ _J. biol. Chem._ 263, 4516–4519

(1988). CAS  PubMed  Google Scholar  * Hemler, M. E., Crouse, C., Takada, Y. & Sonnenberg, A. _J. biol. Chem._ 263, 7660–7665 (1988). CAS  PubMed  Google Scholar  * Bennett, J. S. &

Vilaire, G. _J. clin. Invest._ 64, 1393–1401 (1979). Article  CAS  PubMed  PubMed Central  Google Scholar  * Marguerie, G. A., Edgington, T. S. & Plow, E. F. _J. biol. Chem._ 255,

154–161 (1980). CAS  PubMed  Google Scholar  * Hynes, R. O. _Cell_ 48, 549–554 (1987). Article  CAS  PubMed  Google Scholar  * Ruoslahti, E. & Pierschbacher, M. D. _Science_ 238, 491–497

(1987). Article  ADS  CAS  PubMed  Google Scholar  * Jennings, L. & Phillips, D. R. _J. biol. Chem._ 257, 10458–10463 (1982). CAS  PubMed  Google Scholar  * Pischel, K. D., Bluestein,

H. G. & Woods, V. L. _J. clin. Invest._ 81, 505–513 (1987). Article  Google Scholar  * Pytela, R., Pierschbacher, M. D. & Ruoslahti, E. _Cell_ 40, 191–198 (1985). Article  CAS 

PubMed  Google Scholar  * Takada, Y., Huang, C. & Hemler, M. E. _Nature_ 326, 607–609 (1987). Article  ADS  CAS  PubMed  Google Scholar  * Sonnenberg, A., Janssen, H., Hogervorst, F.,

Calafat, J. & Hilgers, J. _J. biol. Chem._ 262, 10376–10383 (1987). CAS  PubMed  Google Scholar  * Rao, N. C., Barsky, S. H., Terranova, V. P. & Liotta, L. A. _Biochem. biophys. Res.

Commun._ 111, 804–808 (1983). Article  CAS  PubMed  Google Scholar  * Malinoff, H. L. & Wicha, M. S. _J. Cell Biol._ 96, 1475–1479 (1983). Article  CAS  PubMed  Google Scholar  * Lesot,

H., Kühl, U. & von der Mark, K. _EMBO J._ 2, 861–865 (1983). Article  CAS  PubMed  PubMed Central  Google Scholar  * Wayner, E. A. & Carter, W. G. _J. Cell. Biol._ 105, 1873–1884

(1987). Article  CAS  PubMed  Google Scholar  * Graf, J. _et al._ _Cell_ 48, 989–996 (1987). Article  CAS  PubMed  Google Scholar  * Modderman, P. W., Huisman, H. G., van Mourik, J. A. &

von dem Borne, A. E. G. Kr. _Thromb. Haemostas_ 60, 68–74 (1988). Article  CAS  Google Scholar  * Giltay, J. C., Brinkman, H. J. M., Modderman, P. W., von dem Borne, A. E. G. Kr. & van

Mourik, J. A. _Blood_ (submitted). * Cheresh, D. A. _et al._ _J. Cell Biol._ 105, 1163–1173 (1987). Article  CAS  PubMed  Google Scholar  * Edgar, D., Timpl, R. & Thoenen, H. _EMBO J._

3, 1463–1468 (1984). Article  CAS  PubMed  PubMed Central  Google Scholar  * Engvall, E. _et al._ _J. Cell Biol._ 103, 2457–2465 (1986). Article  CAS  PubMed  Google Scholar  * Legrand, C.,

Dubernard, V. & Nurden, A. T. _Biochim. biophys. Acta_ 812, 802–810 (1985). Article  CAS  PubMed  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, The Netherlands Arnoud Sonnenberg 

& Piet W. Modderman * The Netherlands Cancer Institute, Amsterdam, The Netherlands Frans Hogervorst Authors * Arnoud Sonnenberg View author publications You can also search for this

author inPubMed Google Scholar * Piet W. Modderman View author publications You can also search for this author inPubMed Google Scholar * Frans Hogervorst View author publications You can

also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sonnenberg, A., Modderman, P. & Hogervorst, F.

Laminin receptor on platelets is the integrin VLA-6. _Nature_ 336, 487–489 (1988). https://doi.org/10.1038/336487a0 Download citation * Received: 25 July 1988 * Accepted: 11 October 1988 *

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