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ABSTRACT A DECLINE in the T-cell population usually marks the onset of progressive immunological disease in individuals infected with the human immunodeficiency virus (HIV). Because CD4+

cells help to coordinate efficient immune responses, some of the defects in the immune function in advanced cases of AIDS may be explained by the disappearance of these cells. Therefore, an

understanding of the mechanisms used by HIV to induce the reduction of CD4+ cells is important. Here we use a Moloney murine leukaemia virus-based retroviral vector in order to express the

_nef_ gene of HIV-1 in three lymphocytic cell lines expressing CD4. In all cases we find that cell-surface CD4 expression is inversely related to _nef_ expression. However, _nef_ does not

alter steady-state levels of CD4 RNA or CD4 protein. Also, _nef_ can downregulate a CD4 triple mutant (Ser → Ala) that is neither phosphorylated nor down-regulated by phorbol esters,

indicating that _nef_ is acting by a different mechanism. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS

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SIGNALING AND T CELL PROLIFERATION Article Open access 27 August 2024 GFI1 CONTROLS THE FORMATION OF EFFECTOR-LIKE CD8+ T CELLS DURING CHRONIC INFECTION AND CANCER Article Open access 15

May 2025 A CELL-BASED BIOLUMINESCENCE ASSAY REVEALS DOSE-DEPENDENT AND CONTEXTUAL REPRESSION OF AP-1-DRIVEN GENE EXPRESSION BY BACH2 Article Open access 03 November 2020 REFERENCES *

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Center, Seattle, Washington, 98104, USA J. Victor Garcia & A. Dusty Miller Authors * J. Victor Garcia View author publications You can also search for this author inPubMed Google Scholar

* A. Dusty Miller View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE

Garcia, J., Miller, A. Serine phosphorylation-independent downregulation of cell-surface CD4 by _nef_. _Nature_ 350, 508–511 (1991). https://doi.org/10.1038/350508a0 Download citation *

Received: 21 December 1990 * Accepted: 11 February 1991 * Issue Date: 11 April 1991 * DOI: https://doi.org/10.1038/350508a0 SHARE THIS ARTICLE Anyone you share the following link with will

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