Reader TTS

Access through your institution Buy or subscribe The new reports show that actin-based motility only occurs close to the cell periphery, and that the formation of actin tails seems to take

place only during or after fusion of viral particles with the plasma membrane. To reach the cell periphery, however, vaccinia particles travel along microtubules in a saltatory fashion. This

is the first example of a virus using both the microtubule and actin cytoskeleton to spread from cell to cell. Rietdorf and colleagues have identified conventional kinesin as the motor that

is responsible for microtubule-based transport, and show that this motor is recruited to viral particles through the tetratricopeptide (TPR) repeats in the kinesin light chain. The A36R

protein, which is encoded by vaccinia and is essential for vaccinia-induced actin polymerization, is also required to recruit kinesin to virus particles and for their movement to the cell

periphery. Interestingly, Rietdorf _et al_. found that virus particles are never associated with both kinesin and actin tails, indicating that the transition from movement along microtubules

to fusion with the plasma membrane and actin-tail formation is tightly regulated. Whether A36R has any role in this, and the nature of the exact molecular link between A36R and

kinesin-dependent movement, will be the subject of future studies. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution

Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full

article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs *

Contact customer support ORIGINAL RESEARCH PAPER * Ward, B. M. & Moss, B. Visualization of intracellular movement of vaccinia virus virions containing a green fluorescent protein–B5R

membrane protein chimera. _J. Virol._ 75, 4802–4813 (2001) Article  CAS  Google Scholar  * Hollinshead, M. et al. Vaccinia virus ulitilizes microtubules for movement to the cell surface. _J.

Cell Biol._ 154, 389–402 (2001) Article  CAS  Google Scholar  * Rietdorf, J. et al. Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus. _Nature Cell

Biol._ 3, 992–1001 (2001) Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Editor, Nature Cell Biology, Barbara Marte Authors * Barbara Marte

View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Marte, B. Vaccinia

virus on the move. _Nat Rev Mol Cell Biol_ 2, 792 (2001). https://doi.org/10.1038/35099061 Download citation * Issue Date: 01 November 2001 * DOI: https://doi.org/10.1038/35099061 SHARE THIS

ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard

Provided by the Springer Nature SharedIt content-sharing initiative