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ABSTRACT TUBERCULOSIS is responsible for one in four of all avoidable adult deaths in developing countries1. Increased frequency and accelerated fatality of the disease among individuals

infected with human immunodeficiency virus has raised worldwide concern that control programmes may be inadequate2, and the emergence of multidrug-resistant strains of _Mycobacterium

tuberculosis_ has resulted in several recent fatal outbreaks in the United States3. Isonicotinic acid hydrazide (isoniazid, INH) forms the core of antituberculosis regimens; however,

clinical isolates that are resistant to INH show reduced catalase activity and a relative lack of virulence in guinea-pigs4–7. Here we use mycobacterial genetics8,9 to study the molecular

basis of INH resistance. A single _M. tuberculosis_ gene, _katG_, encoding both catalase and peroxidase, restored sensitivity to INH in a resistant mutant of _Mycobacterium smegmatis_, and

conferred INH susceptibility in some strains of _Escherichia coli_. Deletion of _katG_ from the chromosome was associated with INH resistance in two patient isolates of _M. tuberculosis._

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support SIMILAR CONTENT BEING VIEWED BY OTHERS CATALASE ACTIVITY DEFICIENCY SENSITIZES MULTIDRUG-RESISTANT _MYCOBACTERIUM TUBERCULOSIS_ TO THE ATP SYNTHASE INHIBITOR BEDAQUILINE Article Open

access 13 November 2024 CINA MEDIATES MULTIDRUG TOLERANCE IN _MYCOBACTERIUM TUBERCULOSIS_ Article Open access 22 April 2022 CRISPRI CHEMICAL GENETICS AND COMPARATIVE GENOMICS IDENTIFY GENES

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AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * MRC Tuberculosis and Related Infections Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, W12 OHS, UK Ying Zhang & 

Douglas Young * Department of Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, W12 OHS, UK Bryan Allen * Laboratoire de Genetique Moleculaire Bacterienne,

Institut Pasteur, 75724, Paris, 15, France Beate Heym & Stewart Cole * Service de Bacteriologie-Virologie, CHU Pitie-Salpetriere, 75634, Paris, 13, France Beate Heym Authors * Ying Zhang

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Bryan Allen View author publications You can also search for this author inPubMed Google Scholar * Douglas Young View author publications You can also search for this author inPubMed Google

Scholar * Stewart Cole View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE Zhang, Y., Heym, B., Allen, B. _et al._ The catalase—peroxidase gene and isoniazid resistance of _Mycobacterium tuberculosis_. _Nature_ 358, 591–593 (1992).

https://doi.org/10.1038/358591a0 Download citation * Received: 13 May 1992 * Accepted: 02 July 1992 * Issue Date: 13 August 1992 * DOI: https://doi.org/10.1038/358591a0 SHARE THIS ARTICLE

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