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Access through your institution Buy or subscribe Even before the first member of the NF-κB family was cloned, it was recognized that the association of IκB with NF-κB is controlled by

phosphorylation, and thus the search for the protein kinase responsible began. On page 548of this issue3, DiDonato_et al_. report the purification and cloning of a cytokine-responsive IκB

kinase which might put an end to this long and frustrating search. In 1990, Ghosh and Baltimore4 demonstrated that treatment of a partially purified NF-κB/IκB complex by certain kinases

could induce dissociation of the complex and activation of NF-κB DNA-binding activity. Cloning5 of the complementary DNA encoding IκBα, one of the three forms of IκB now known, then

stimulated an intense burst of research which to some extent clarified the signalling events. In response to extracellular stimuli, the IκBα molecule becomes phosphorylated by a

serine-specific kinase at positions 32 and 36. This phosphorylation in turn induces polyubiquitination of the molecule, which targets it for rapid degradation (and not for dissociation from

NF-κB, as originally postulated)2. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51

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to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES *

Sen, R. & Baltimore, D. _Cell_ 47, 921–928 (1986). Google Scholar  * Baldwin, A. S. _Annu. Rev. Immunol_. 14, 649–683 (1996). Google Scholar  * DiDonato, J. A., Hayakawa, M., Rothwarf,

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499–512 (1994). Google Scholar  * Schouten, G. J._et al._ _EMBO J._ 16, 3133–3144 (1997). Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Unité de Biologie

Molculaire de l'Expression Gnique, UMR 321 CNRS, Institut Pasteur, 25 rue du Dr Roux, Paris, 75724 Cedex 15, France Alain Israë Authors * Alain Israë View author publications You can

also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Israë, A. IκB kinase all zipped up. _Nature_ 388,

520–521 (1997). https://doi.org/10.1038/41433 Download citation * Issue Date: 07 August 1997 * DOI: https://doi.org/10.1038/41433 SHARE THIS ARTICLE Anyone you share the following link with

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