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ABSTRACT 2-Chlorodeoxyadenosine (2-CDA) is an adenosine deaminase resistant analogue of deoxyadenosine which has shown clinical activity in human hematologic neoplasms. The exact mode of

action of this drug remains the subject of investigation. We applied the Differential Staining Cytotoxicity (DiSC) assay to 50 human tumour specimens obtained from patients with a variety of

hematologic malignancies to characterise the activity spectrum of 2-CDA. We evaluated the disease-specific activity of this agent in vitro and compared its relative cytotoxicity with that

of other antineoplastic agents in current clinical use. Comparisons were conducted against nitrogen mustard, doxorubicin, vincristine and cytosine arabinoside. Our results indicate that

2-CDA has activity in myeloid and many lymphoid neoplasms but that multiple myeloma specimens reveal significant resistance. Cross resistance studies reveal a correlation between 2-CDA and

the alkylator nitrogen mustard but no correlation between 2-CDA and doxorubicin, vincristine nor cytosine arabinoside. The results suggest 2-CDA activity in many human hematologic neoplasms

with the clear exception of multiple myeloma and further suggest a relationship between this agent and alkylators of the mustard class. The DiSC assay may provide useful insights in the

pre-clinical evaluation of new antineoplastic drugs and may help to elucidate drug activities and mechanisms of action. Access through your institution Buy or subscribe This is a preview of

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SUPERIOR TO CONVENTIONAL DRUG SENSITIVITY ASSAYS IN DETECTING CYTOTOXIC DRUGS Article Open access 26 May 2020 STANDARDIZED ASSAYS TO MONITOR DRUG SENSITIVITY IN HEMATOLOGIC CANCERS Article

Open access 01 December 2023 QUINACRINE-CASIN COMBINATION OVERCOMES CHEMORESISTANCE IN HUMAN ACUTE LYMPHOID LEUKEMIA Article Open access 26 November 2021 AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * Memorial Cancer Institute, Long Beach, 90806, California RA Nagourney Authors * RA Nagourney View author publications You can also search for this author inPubMed Google

Scholar * SS Evans View author publications You can also search for this author inPubMed Google Scholar * JC Messenger View author publications You can also search for this author inPubMed 

Google Scholar * YZ Su View author publications You can also search for this author inPubMed Google Scholar * LM Weisenthal View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Nagourney, R., Evans, S., Messenger, J. _et al._ 2 chlorodeoxyadenosine activity

and cross resistance patterns in primary cultures of human hematologic neoplasms. _Br J Cancer_ 67, 10–14 (1993). https://doi.org/10.1038/bjc.1993.3 Download citation * Issue Date: 01

January 1993 * DOI: https://doi.org/10.1038/bjc.1993.3 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable

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