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SUMMARY To obtain cosmid markers and transcribed sequences from a specific chromosome region, a series of radiation-reduced hybrids (RHs) containing various regions of human chromosome 11

was prepared from microcell hybrid A9 (_neo_11) cells containing a normal human chromosome 11 tagged with pSV2_neo_ at 11p11.2. Among 15 radiation hybrid clones isolated, RH(11)-9 which

contains a q23 fragment in addition to the_neo_ integration site, was used for the construction of a cosmid library. Cosmid clones having human DNA sequences were screened, and localized by

Southern hybridization with the radiation hybrid panel. Fifty-nine cosmids were assigned to 11q23 and 6 cosmids to 11p11.2. Exon amplification proceeded with 23 of the 59 cosmids and 16

putative exons were cloned. Three of them were identical to those constituting a known gene which locates on q23 (ATDC), and the others were unknown. Thus, the RHs containing various

subchromosomal fragments of chromosome 11 were useful for constructing region-specific DNA markers. The RH-(11)-9 cells and putative exons also facilitate the positional cloning of genes in

the 11q23 region. SIMILAR CONTENT BEING VIEWED BY OTHERS THE GENOMIC STRUCTURE OF A HUMAN CHROMOSOME 22 NUCLEOLAR ORGANIZER REGION DETERMINED BY TAR CLONING Article Open access 04 February

2021 ASSEMBLY OF 43 HUMAN Y CHROMOSOMES REVEALS EXTENSIVE COMPLEXITY AND VARIATION Article 23 August 2023 EXPERIMENTAL METHOD FOR HAPLOTYPE PHASING ACROSS THE ENTIRE LENGTH OF CHROMOSOME 21

IN TRISOMY 21 CELLS USING A CHROMOSOME ELIMINATION TECHNIQUE Article Open access 31 May 2022 ARTICLE PDF REFERENCES * Akao Y, Seto M, Yamamoto K, Iida S, Nakazawa S, Inazawa J, Abe T,

Takahashi T, Ueda R (1992): The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL-1 gene with t(4;11) and t(11;19) translocation. Cancer Res52:6083–6087 CAS 

PubMed  Google Scholar  * Buckler AJ, Chang DD, Graw SL, Brook D, Haber DA, Sharp PA, Housman DE (1991): Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc

Natl Acad Sci USA88:4005–4009 Article  CAS  PubMed  PubMed Central  Google Scholar  * Call KM, Glaser T, Ito CY, Buckler AJ, Pelletier J, Haber DA, Rose EA, Kral A, Yeger H, Lewis WH, Jones

C, Housman DE (1990): Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus. Cell60:509–520 Article  CAS  PubMed  Google

Scholar  * Carter SL, Negrini M, Baffa R, Gillum DR, Rosenberg AL, Schwartz GF, Croce CM (1994): Loss of heterozygosity at 11q22-q23 in breast cancer. Cancer Res54:6270–6274 CAS  PubMed 

Google Scholar  * Chen Z, Brand NJ, Chen A, Chen S-J, Tong J-H, Wang Z-Y, Waxman S, Zelent A (1993): Fusion between a novel Kruppel-like zinc finger gene and retinoic acid receptor-α locus

due to a variant t(11:17) translocation associated with acute promyelocytic leukemia. EMBO J12:1161–1167 CAS  PubMed  PubMed Central  Google Scholar  * Cherif D, Der-Sarkissan H, Derre J,

Tokino T, Nakamura Y, Berger R (1992): The 11q23 breakpoint in acute leukemia with t(11;19)(q23;p13) is distal to those of t(4;11), t(6;11) and t(9;11). Genes Chromosomes Cancer4:107–112

Article  CAS  PubMed  Google Scholar  * Chomczynski P, Sacchi N (1987): Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal

Biochem162:156–159 Article  CAS  PubMed  Google Scholar  * Devilee P, Van Schothorst EM, Bardoel AFJ, Bonsing B, Kuipers-Dijkshoorn N, James MR, Freuren G, Van der may AGL, Cornelisse CJ

(1994): Allelotype of head and neck paragangliomas: allelic imbarance is confined to the long arm of chromosome 11, the site of the predisposing locus PGL. Genes Chromosomes Cancer11:71–78

Article  CAS  PubMed  Google Scholar  * Emi M, Takahashi E, Koyama K, Okui K, Oshimura M, Nakamura Y (1992): Isolation and mapping of 88 new RFLP markers on human chromosome 8.

Genomics13:1261–1266 Article  CAS  PubMed  Google Scholar  * Foulkes WD, Champbell IG, Stamp GW, Trowsdale J (1993): Loss of heterozygosity and amplification on chromosome 11q in human

ovalian cancer. Br J Cancer67:268–273 Article  CAS  PubMed  PubMed Central  Google Scholar  * Gatti R (1991): Localizing the genes for Ataxia-telangiectasia: a human models for inherited

cancer susceptibility. Adv Cancer Res56:77–104 Article  CAS  PubMed  Google Scholar  * Gerhard DS, Lawrence E, Wu J, Chua H, Ma N, Bland S, Jones C (1992): Isolation of 1001 new markers from

human chromosome 11, excluding the region of 11q13-p15.5, and their sublocalization by a new series of radiation-reduced somatic cell hybrids. Genomics13:1133–1142 Article  CAS  PubMed 

Google Scholar  * Gillet GT, McConville CM, Byrd PJ, Stankovic T, Taylor AM, Hunt DM, West LF, Fox MF, Povey S, Benham F (1993): Irradiation hybrids for human chromosome 11: characterization

and use for generating region-specific markers in 11q14-q23. Genomics15:332–341 Article  Google Scholar  * Hampton GM, Penny LA, Baergen RN, Larson A, Brewer C, Liao S, Busby-Earle RMC,

Williams AWR, Steel CM, Bird CC, Stanbridge EJ, Evans GA (1994): Loss of heterozygosity in cervical carcinoma: subchromosomal localization of a putative tumor-suppressor gene to chromosome

11q22-q24. Proc Natl Acad Sci USA91:6953–6957 Article  CAS  PubMed  PubMed Central  Google Scholar  * Heutink P, Van der May AGL, Sandkuijl LA, Van Gils APG, Bardoel A, Breedvald GJ, Van

Vliet M, Van Ommen GJB, Cornelisse CJ, Oostra BA, Weber JL, Devilee P (1992): A gene subject to genomic imprinting and responsible for hereditary paragangliomas maps to chromosome

11q23-qter. Hum Mol Genet1:7–10 Article  CAS  PubMed  Google Scholar  * Hori T, Takahashi E, Tanigami A, Tokino T, Nakamura Y (1992): A high-resolution cytogenetic map of 186 cosmid DNA

markers for human chromosome 11. Genomics13:129–133 Article  CAS  PubMed  Google Scholar  * Kapp LN, Painter RB, Yu LC, Van Loon N, Richard CW III, James MR, Cox DR, Murane JP (1992):

Cloning of a candidate gene for Ataxia-telangiectasia group D. Am J Hum Genet51: 45–54 CAS  PubMed  PubMed Central  Google Scholar  * Keldysh PL, Dragani TA, Fleischman EW, Konstantinova LN,

Perevoschikov AG, Pierotti MA, Della Porta G, Kopnin BP (1993): 11q deletions in human colorectal carcinomas: cytogenetics and RFLP analysis. Genes Chromosomes Cancer6:45–50 Article  CAS 

PubMed  Google Scholar  * Kobayashi H, Episona R III, Fernand AA, Bogy C, Diaz MO, LeBeou MM, Rowly JD (1993): Analyses of deletions of the long arm of chromosome 11 in hematologic

malignancies with_FISH_. Genes Chromosomes Cancer8:246–252 Article  CAS  PubMed  Google Scholar  * Koi M, Shimizu M, Morita H, Yamada H, Oshimura M (1989): Construction of mouse A9 clones

containing a single chromosome tagged with neomycin-resistance gene_via_ microcell fusion. Jpn J Cancer Res80:413–418 Article  CAS  PubMed  Google Scholar  * Kugoh H, Nakagawa Y, Mitsuya K,

Mita T, Suzuki M, Suzuki N, Uejima H, Yuasa Y, Oshimura M (1995): Isolation and mapping of 186 new DNA markers on human chromosome 1. Genomics27:207–210 Article  CAS  PubMed  Google Scholar

  * Kurimasa A, Nagata Y, Shimizu M, Emi M, Nakamura Y, Oshimura M (1994): A human gene that restores the DNA-repair defect in SCID mice is located on 8p11.1-q11.1. Hum Genet93:21–26 Article

  CAS  PubMed  Google Scholar  * Leach RJ, Thayer MJ, Chafer AJ, Fournier REK (1989): Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. Genomics5:167–176

Article  CAS  PubMed  Google Scholar  * Ledbetter SA, Nelson DL, Warren ST, Ledbetter DH (1990): Rapid isolation of DNA probes within chromosome regions by interspersed repetitive sequence

polymerase chain reaction. Genomics6:475–481 Article  CAS  PubMed  Google Scholar  * Leonhardt EA, Kapp LN, Young BR, Murane JP (1994): Cloning of a candidate gene for Ataxiatelangiectasia

group D. Genomics19:130–136 Article  CAS  PubMed  Google Scholar  * Monaco AP, Neve RL, Colleti-Feener C, Bertelson CJ, Kurnit DM, Kunkel LM (1986): Isolation of candidate cDNAs for portions

of the Duchenne muscular distrophy gene. Nature323:646–650 Article  CAS  PubMed  Google Scholar  * Page DC, Mosher R, Simpson EM, Fisher EMC, Mardon G, Pollac J, McGillivray B, de la

Chapelle A, Brown LG (1987): The sex-determining region of the human Y chromosome encodes a finger protein. Cell51:1091–1104 Article  CAS  PubMed  Google Scholar  * Rabbits TH (1994):

Chromosomal translocations in human cancer. Nature372:134–149 Article  Google Scholar  * Rommens JM, Iannuzzi MC, Kerem BS, Drumm ML, Melmer G, Dean M, Rozmahel R, Cole JL, Kennedy D, Hidaka

N, Buchwald M, Riordan JR, Tsui LC, Collins FS (1989): Identification of the cystic fibrosis gene: chromosome walking and jumping. Science245:1059–1065 Article  CAS  PubMed  Google Scholar

  * Sanger F, Nicklen S, Coulson AR (1977): DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA74:5463–5467 Article  CAS  PubMed  PubMed Central  Google Scholar  * Sobel

E, Lange E, Jaspers NGJ, Chessa L, Sanal O, Shiloh Y, Taylor AMR, Weemaes CM, Lange K, Gatti RA (1992): Ataxia-telangiectasia: linkage evidence for genetic heterogeneity. Am J Hum

Genet50:1343–1348 CAS  PubMed  PubMed Central  Google Scholar  * Takahashi E, Hori T, O'Connell P, Leppert M, White R (1990): R-banding and nonisotpoic_in situ_ hybridization: precise

localization of the human type II collagen (Co12A1). Hum Genet86:14–16 Article  CAS  PubMed  Google Scholar  * Takahashi E, Yamakawa K, Nakamura Y, Hori T (1992): A high resolution

cytogenetic map of human chromosome 3: localization of 291 new cosmid markers by direct R-banding fluorescence_in situ_ hybridization. Genomics13:1047–1055 Article  CAS  PubMed  Google

Scholar  * Tamari M, Hamaguchi M, Shimizu M, Oshimura M, Takayama H, Kohno T, Yamaguchi N, Sugimura T, Terada M, Yokota J (1992). Ordering of human chromosome 3p markers by radiation hybrid

mapping. Genomics13:705–712 Article  CAS  PubMed  Google Scholar  * Tokino T, Takahashi E, Mori M, Tanigami A, Glaser T, Park JW, Jones C, Hori T, Nakamura Y (1991): Isolation and mapping of

62 new RFLP markers on human chromsome 11. Am J Hum Genet48:258–262 CAS  PubMed  PubMed Central  Google Scholar  * Tomlinson IPM, Gammack AJ, Sticland JE, Mann GJ, Mackie RM, Kefford RF,

McGee JO (1993): Loss of heterozygosity in malignant melanoma at loci on chromosome 11 and 17 implicated in the pathogenesis of other cancers. Genes Chromosomes Cancer7:169–172 Article  CAS

  PubMed  Google Scholar  * Ziemin S, McCabe NR, Gill HJ, Episona R III, Patel Y, Harden A, Rubinelli P, Smith SD, LeBeau MM, Rowley JD, Diaz MO (1991). Identification of a gene, MLL, that

spans the breakpoint in 11q23 translocations associated with human leukemias. Proc Natl Acad Sci USA88:10735–10739 Article  Google Scholar  * Zoghbi HY, McCall AE, LeBorgne-Demarquoy F

(1991): Sixty-five radiation hybrids for the short arm of human chromosome 6: their value as a mapping panel and a source for rapid isolation of new probes using repeat element-mediated PCR.

Genomics9:713–720 Article  CAS  PubMed  Google Scholar  Download references AUTHOR INFORMATION Author notes * Yuzuki Nakagawa Present address: Hatano Research Institute/Food and Drug Safety

Center, 729-5 Ochiai, 257, Hadano, Kanagawa, Japan AUTHORS AND AFFILIATIONS * Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, 86

Nishimachi, 683, Yonago, Tottori, Japan Motonobu Katoh, Yuzuki Nakagawa, Toshio Yawata, Satoshi Kumano, Eisuke Kobayashi, Akihiro Kurimasa, Hiroyuki Kugoh & Mitsuo Oshimura Authors *

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T. _et al._ Cosmids and transcribed sequences from chromosome 11q23. _Jap J Human Genet_ 40, 307–317 (1995). https://doi.org/10.1007/BF01900597 Download citation * Received: 18 May 1995 *

Accepted: 16 August 1995 * Published: 01 December 1995 * Issue Date: December 1995 * DOI: https://doi.org/10.1007/BF01900597 SHARE THIS ARTICLE Anyone you share the following link with will

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content-sharing initiative KEY WORDS * chromosome 11q23 * radiation-reduced hybrid * cosmids * exon amplification * transcribed sequences