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ABSTRACT We have designed an advanced δ–integration system (integration of genes into the δ–sequence of yeast retrotransposon Ty) and used it for secretion of human nerve growth factor

(hNGF) from _Saccharomyces cerevisiae_. The expression and secretion of hNGF was directed by the PGK promoter and _MFα1_ prepro–signal. Using two selectable markers (_URA3_ and _leu2–d_),

haploid yeast strains were constructed with approximately 20 copies of a δ–integrated hNGF expression cassette on four chromosomes. The strain secreted hNGF at levels 3–4 fold higher than a

2μm–based plasmid. Northern and Western analyses revealed that the oversecretion was caused by an increased amount of mRNA. We also detected an unusual processing of the _MFα1_ prepro–hNGF

fusion protein that required the _pep4_ mutation. Application of this system for industrial purposes is discussed. Access through your institution Buy or subscribe This is a preview of

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FOR CHROMOSOMAL INTEGRATION IN FISSION YEAST Article Open access 08 June 2023 OPENPICHIA: LICENCE-FREE _KOMAGATAELLA PHAFFII_ CHASSIS STRAINS AND TOOLKIT FOR PROTEIN EXPRESSION Article Open

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Download references AUTHOR INFORMATION Author notes * Akira Sakai: Corresponding author. AUTHORS AND AFFILIATIONS * Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life

Sciences, 11, Minamiooya, Machida-shi, Tokyo, 194, Japan Akira Sakai, Fumiko Ozawa, Takako Higashizaki, Yuki Shimizu & Fumio Hishinuma Authors * Akira Sakai View author publications You

can also search for this author inPubMed Google Scholar * Fumiko Ozawa View author publications You can also search for this author inPubMed Google Scholar * Takako Higashizaki View author

publications You can also search for this author inPubMed Google Scholar * Yuki Shimizu View author publications You can also search for this author inPubMed Google Scholar * Fumio Hishinuma

View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sakai, A., Ozawa,

F., Higashizaki, T. _et al._ Enhanced Secretion of Human Nerve Growth Factor from _Saccharomyces cerevisiae_ using an Advanced δ–Integration System. _Nat Biotechnol_ 9, 1382–1385 (1991).

https://doi.org/10.1038/nbt1291-1382 Download citation * Received: 26 June 1991 * Accepted: 14 August 1991 * Issue Date: 01 December 1991 * DOI: https://doi.org/10.1038/nbt1291-1382 SHARE

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