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ABSTRACT The F-actin based motor protein myosin II has a key role in cytokinesis. Here we show that the _Schizosaccharomyces pombe_ regulatory light chain (RLC) protein Rlc1p binds to Myo2p

in manner that is dependent on the IQ sequence motif (the RLC-binding site), and that Rlc1p is a component of the actomyosin ring. Rlc1p is important for cytokinesis at all growth

temperatures and is essential for this process at lower temperatures. Interestingly, all deleterious phenotypes associated with the loss of Rlc1p function are suppressed by deletion of the

RLC binding site on Myo2p. We conclude that the sole essential function of RLCs in fission yeast is to relieve the auto-inhibition of myosin II function, which is mediated by the RLC-binding

site, on the myosin heavy chain (MHC). Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through

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Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS HOW MYOSIN VI TRAPS ITS OFF-STATE, IS ACTIVATED AND DIMERIZES Article Open access 23 October 2023

AUTOINHIBITION AND ACTIVATION OF MYOSIN VI REVEALED BY ITS CRYO-EM STRUCTURE Article Open access 08 February 2024 UNC-45 ASSISTED MYOSIN FOLDING DEPENDS ON A CONSERVED FX3HY MOTIF IMPLICATED

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Enzymol._ 283, 494–506 (1997). Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank T. Pollard for the _myp2_-null strain, and P. Silver and K. Sawin for anti-GFP

antibodies. We also thank A. Munn, S. Oliferenko, K. Sampath and all members of the IMA yeast laboratories (especially S. Naqvi and V. Rajagopalan) for discussion and critical reading of the

manuscript. Rlc1p was independently isolated by V. Simanis and colleagues, whom we thank for sharing unpublished information. This work was supported by research funds from the National

Science and Technology Board, Singapore. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Cell Division Laboratory, Institute of Molecular Agrobiology, The National University of Singapore, 1

Research Link, 117604, Singapore Naweed I. Naqvi, Kelvin C. Y. Wong, Xie Tang & Mohan K. Balasubramanian Authors * Naweed I. Naqvi View author publications You can also search for this

author inPubMed Google Scholar * Kelvin C. Y. Wong View author publications You can also search for this author inPubMed Google Scholar * Xie Tang View author publications You can also

search for this author inPubMed Google Scholar * Mohan K. Balasubramanian View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR

Correspondence to Mohan K. Balasubramanian. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Naqvi, N., Wong, K., Tang, X. _et al._ Type II myosin

regulatory light chain relieves auto-inhibition of myosin-heavy-chain function. _Nat Cell Biol_ 2, 855–858 (2000). https://doi.org/10.1038/35041107 Download citation * Received: 11 May 2000

* Revised: 17 July 2000 * Accepted: 01 August 2000 * Published: 17 October 2000 * Issue Date: November 2000 * DOI: https://doi.org/10.1038/35041107 SHARE THIS ARTICLE Anyone you share the

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