Reader TTS

ABSTRACT The rapid growth in ubiquitin biology requires facile chemical approaches for protein ubiquitylation that can overcome the common problem of low yield faced by the enzymatic

reaction catalyzed by ubiquitin ligases. We report a chemical approach for monoubiquitylation and SUMOylation of PCNA through disulfide exchange and intein chemistry. We used the chemically

ubiquitylated and SUMOylated PCNAs in studying translesion DNA synthesis and revealed a surprising degree of flexibility of the ubiquitin modification. Access through your institution Buy or

subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online

access $259.00 per year only $21.58 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which

are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS

A MODULAR TOOLBOX TO GENERATE COMPLEX POLYMERIC UBIQUITIN ARCHITECTURES USING ORTHOGONAL SORTASE ENZYMES Article Open access 11 November 2021 THIOETHER-MEDIATED PROTEIN UBIQUITINATION IN

CONSTRUCTING AFFINITY- AND ACTIVITY-BASED UBIQUITINATED PROTEIN PROBES Article 25 April 2025 STRUCTURAL BASIS FOR TRANSTHIOLATION INTERMEDIATES IN THE UBIQUITIN PATHWAY Article Open access

14 August 2024 REFERENCES * Kerscher, O., Felberbaum, R. & Hochstrasser, M. _Annu. Rev. Cell Dev. Biol._ 22, 159–180 (2006). Article  CAS  Google Scholar  * Branzei, D. & Foiani, M.

_Nat. Rev. Mol. Cell Biol._ 9, 297–308 (2008). Article  CAS  Google Scholar  * Bergink, S. & Jentsch, S. _Nature_ 458, 461–467 (2009). Article  CAS  Google Scholar  * Ulrich, H.D. _DNA

Repair (Amst.)_ 8, 461–469 (2009). Article  CAS  Google Scholar  * Lehmann, A.R. et al. _DNA Repair (Amst.)_ 6, 891–899 (2007). Article  CAS  Google Scholar  * Pickart, C.M. _Annu. Rev.

Biochem._ 70, 503–533 (2001). Article  CAS  Google Scholar  * McGinty, R.K., Kim, J., Chatterjee, C., Roeder, R.G. & Muir, T.W. _Nature_ 453, 812–816 (2008). Article  CAS  Google Scholar

  * Krishna, T.S., Kong, X.P., Gary, S., Burgers, P.M. & Kuriyan, J. _Cell_ 79, 1233–1243 (1994). Article  CAS  Google Scholar  * Moldovan, G.L., Pfander, B. & Jentsch, S. _Cell_

129, 665–679 (2007). Article  CAS  Google Scholar  * Chang, D.J. & Cimprich, K.A. _Nat. Chem. Biol._ 5, 82–90 (2009). Article  CAS  Google Scholar  * Hoege, C., Pfander, B., Moldovan,

G.L., Pyrowolakis, G. & Jentsch, S. _Nature_ 419, 135–141 (2002). Article  CAS  Google Scholar  * Stelter, P. & Ulrich, H.D. _Nature_ 425, 188–191 (2003). Article  CAS  Google

Scholar  * Garg, P. & Burgers, P.M. _Proc. Natl. Acad. Sci. USA_ 102, 18361–18366 (2005). Article  CAS  Google Scholar  * Haracska, L., Unk, I., Prakash, L. & Prakash, S. _Proc.

Natl. Acad. Sci. USA_ 103, 6477–6482 (2006). Article  CAS  Google Scholar  * Zhuang, Z. et al. _Proc. Natl. Acad. Sci. USA_ 105, 5361–5366 (2008). Article  CAS  Google Scholar  * Kannouche,

P.L., Wing, J. & Lehmann, A.R. _Mol. Cell_ 14, 491–500 (2004). Article  CAS  Google Scholar  * Watanabe, K. et al. _EMBO J._ 23, 3886–3896 (2004). Article  CAS  Google Scholar  * Plosky,

B.S. et al. _EMBO J._ 25, 2847–2855 (2006). Article  CAS  Google Scholar  * Parker, J.L., Bielen, A.B., Dikic, I. & Ulrich, H.D. _Nucleic Acids Res._ 35, 881–889 (2007). Article  CAS 

Google Scholar  * Indiani, C., McInerney, P., Georgescu, R., Goodman, M.F. & O'Donnell, M. _Mol. Cell_ 19, 805–815 (2005). Article  CAS  Google Scholar  * Heltzel, J.M., Maul, R.W.,

Scouten Ponticelli, S.K. & Sutton, M.D. _Proc. Natl. Acad. Sci. USA_ 106, 12664–12669 (2009). Article  CAS  Google Scholar  * Bienko, M. et al. _Science_ 310, 1821–1824 (2005). Article

  CAS  Google Scholar  * Freudenthal, B.D., Ramaswamy, S., Hingorani, M.M. & Washington, M.T. _Biochemistry_ 47, 13354–13361 (2008). Article  CAS  Google Scholar  * van Kasteren, S.I. et

al. _Nature_ 446, 1105–1109 (2007). Article  CAS  Google Scholar  * Gamblin, D.P. et al. _Mol. Biosyst._ 4, 558–561 (2008). Article  CAS  Google Scholar  Download references

ACKNOWLEDGEMENTS We thank S. Prakash (University of Texas Medical Branch) for the generous gift of yeast Polδ. We also thank C. Thorpe for helpful discussions. This work was supported in

part by a grant from the University of Delaware Research Foundation to Z.Z. (UDRF08000654). L.H. acknowledges funding from the Hungarian Science Foundation (OTKA 77495-TAMOP-4.2.2/08/1).

AUTHOR INFORMATION Author notes * Junjun Chen and Yongxing Ai: These authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Department of Chemistry and Biochemistry, University

of Delaware, Newark, Delaware, USA Junjun Chen, Yongxing Ai, Jialiang Wang & Zhihao Zhuang * Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged,

Temesvari, Hungary Lajos Haracska Authors * Junjun Chen View author publications You can also search for this author inPubMed Google Scholar * Yongxing Ai View author publications You can

also search for this author inPubMed Google Scholar * Jialiang Wang View author publications You can also search for this author inPubMed Google Scholar * Lajos Haracska View author

publications You can also search for this author inPubMed Google Scholar * Zhihao Zhuang View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS

J.C., Y.A. and Z.Z. designed research; J.C., Y.A. and J.W. performed research; L.H. contributed new reagents; J.C., Y.A. and Z.Z. analyzed data; and Z.Z. wrote the paper. CORRESPONDING

AUTHOR Correspondence to Zhihao Zhuang. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND FIGURES

Supplementary Methods and Supplementary Figures 1–19 (PDF 998 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Chen, J., Ai, Y., Wang, J. _et al._

Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis. _Nat Chem Biol_ 6, 270–272 (2010). https://doi.org/10.1038/nchembio.316 Download citation * Received: 29

September 2009 * Accepted: 04 December 2009 * Published: 07 March 2010 * Issue Date: April 2010 * DOI: https://doi.org/10.1038/nchembio.316 SHARE THIS ARTICLE Anyone you share the following

link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature

SharedIt content-sharing initiative