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ABSTRACT The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a

Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of _Swap70__−/−_ B cells into lymph nodes _in vivo_. _Swap70__−/−_ B

cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, _Swap70__−/−_ B cells

aberrantly regulated integrin-mediated adhesion. During attachment, _Swap70__−/−_ B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined

region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes. Access through your institution Buy or subscribe This is a preview of subscription content,

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the B cell antigen receptor complex. _Proc. Natl. Acad. Sci. USA_ 97, 2180–2184 (2000). Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank L. Quemeneur for

discussions, and A. Berg and M. Chopin for help. Supported by the National Institutes of Health (AI49470 to R.J.) and the Deutsche Forschungsgemeinschaft (SFB605 to R.J.). AUTHOR INFORMATION

AUTHORS AND AFFILIATIONS * Institute of Physiological Chemistry, Dresden University of Technology, Dresden, 01307, Germany Glen Pearce & Rolf Jessberger * Department of Gene and Cell

Medicine, Mount Sinai School of Medicine, New York, 10029, New York, USA Veronique Angeli, Gwendalyn J Randolph & Rolf Jessberger * Center for Blood Research, Harvard Medical School,

Boston, 02115, Massachusetts, USA Tobias Junt & Ulrich von Andrian * Institute of Physiology, Dresden University of Technology, Dresden, 01307, Germany Hans-Joachim Schnittler Authors *

Glen Pearce View author publications You can also search for this author inPubMed Google Scholar * Veronique Angeli View author publications You can also search for this author inPubMed 

Google Scholar * Gwendalyn J Randolph View author publications You can also search for this author inPubMed Google Scholar * Tobias Junt View author publications You can also search for this

author inPubMed Google Scholar * Ulrich von Andrian View author publications You can also search for this author inPubMed Google Scholar * Hans-Joachim Schnittler View author publications

You can also search for this author inPubMed Google Scholar * Rolf Jessberger View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR

Correspondence to Rolf Jessberger. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY FIG. 1

Inflammation-induced migration of B cells into the peritoneum. (PDF 207 kb) SUPPLEMENTARY FIG. 2 Flow cytometric analysis of integrin and chemokine receptor expression on purified B cells.

(PDF 692 kb) SUPPLEMENTARY FIG. 3 Actin polymerization and Rac-1 activity and localization. (PDF 531 kb) SUPPLEMENTARY VIDEO 1 WT B cells moving on an anti-CD44 coated surface. Images were

collected every 10 sec for 20 min after a 25 min attachment period. (MOV 3624 kb) SUPPLEMENTARY VIDEO 2 _Swap70__−/−_ B cells moving on an anti-CD44 coated surface. Images were collected

every 10 sec for 20 min after a 25 min attachment period. (MOV 3100 kb) SUPPLEMENTARY METHODS (PDF 73 KB) SUPPLEMENTARY NOTE (PDF 66 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT

THIS ARTICLE CITE THIS ARTICLE Pearce, G., Angeli, V., Randolph, G. _et al._ Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs. _Nat Immunol_ 7, 827–834

(2006). https://doi.org/10.1038/ni1365 Download citation * Received: 03 February 2006 * Accepted: 19 June 2006 * Published: 16 July 2006 * Issue Date: 01 August 2006 * DOI:

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