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ABSTRACT Patients with B-cell chronic lymphocytic leukemia (CLL) acquire an immunodeficiency with many characteristics similar to those of persons with inherited defects in the gene encoding

the CD40-ligand (CD154). We found that the blood and splenic CD4+ T cells of patients with CLL failed to express surface CD154 after CD3 ligation. However, using an enzyme-linked

immunosorbent assay (ELISA)-based quantitative competitive polymerase chain reaction (PCR), we noted that CD3 ligation could induce such T cells to express CD154 messenger RNA at levels

similar to that of CD3-activated T cells from normal donors. Moreover, addition of increasing numbers of CLL B cells to activated normal donor T cells rapidly resulted in progressively

greater down-modulation of CD154. Such down-modulation of CD154 could be blocked by addition of CD40 monoclonal antibody to cultures _in vitro_. We propose that leukemia cell-mediated

down-modulation of CD154 on activated T cells accounts for some of the acquired immune defects of patients with CLL. Access through your institution Buy or subscribe This is a preview of

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ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS T CELLS TARGETED TO TDT KILL

LEUKEMIC LYMPHOBLASTS WHILE SPARING NORMAL LYMPHOCYTES Article Open access 06 December 2021 DUAL T-CELL CONSTANT Β CHAIN (TRBC)1 AND TRBC2 STAINING FOR THE IDENTIFICATION OF T-CELL NEOPLASMS

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interleukin 12 secretion. _J. Exp. Med._ 183, 693–698 (1996). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Hematology/Oncology,

Department of Medicine, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, California, 92093-0663, USA Mark Cantwell, Tinh Hua, Jim Pappas & Thomas J. Kipps Authors * Mark Cantwell

View author publications You can also search for this author inPubMed Google Scholar * Tinh Hua View author publications You can also search for this author inPubMed Google Scholar * Jim

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Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Cantwell, M., Hua, T., Pappas, J. _et al._ Acquired CD40-ligand deficiency in chronic lymphocytic

leukemia. _Nat Med_ 3, 984–989 (1997). https://doi.org/10.1038/nm0997-984 Download citation * Received: 07 January 1997 * Accepted: 18 June 1997 * Issue Date: 01 September 1997 * DOI:

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