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Access through your institution Buy or subscribe > However, α-PD1 was then transferred to neighbouring TAMs within > minutes, where it was retained. To gain insight into the

pharmacokinetics of PD1 inhibitors and to investigate potential mechanisms of resistance, Pittet and colleagues followed labelled monoclonal antibodies (mAbs) targeting PD1 (α-PD1) in real

time and analysed their interaction with different cell types in the tumour microenvironment _in vivo_ in mice carrying xenografts derived from the MC38 colon cancer cell line. They observed

that the drug starts accumulating in tumours within minutes after injection and achieves maximum concentration after 24 h. Reporter mice with labelled T cells and TAMs showed tagged α-PD1

on T cells as early as 5 minutes after injection with a pericellular distribution. However, α-PD1 was then transferred to neighbouring TAMs within minutes, where it was retained. This

phenomenon was also observed in mouse models of melanoma and adenocarcinoma. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your

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REFERENCES * Arlauckas, S. P. et al. _In vivo_ imaging reveals a tumor-associated macrophage-mediated resistance pathway in anti-PD-1 therapy. _Sci. Transl Med._ 9, eaal3604 (2017) Article 

Google Scholar  * Gordon, S. R. et al. PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity. _Nature_, 545, 495–499 (2017) Article  CAS  Google Scholar

  Download references Authors * M. Teresa Villanueva View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Villanueva, M. Macrophages steal the show. _Nat Rev Drug Discov_ 16, 455 (2017). https://doi.org/10.1038/nrd.2017.126 Download citation * Published: 29

June 2017 * Issue Date: July 2017 * DOI: https://doi.org/10.1038/nrd.2017.126 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

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