Connecting genotypes and phenotypes

Connecting genotypes and phenotypes


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Access through your institution Buy or subscribe Given the large fraction of genes of unknown function that is found in most sequenced genomes, developing high-throughput approaches to


assign genotypes to a particular phenotype is a priority. One approach involves determining the effect of a defined environmental condition on growth rate and fitness for


single-gene-knockout libraries; however, such genome-wide libraries are available for only a handful of organisms. These authors instead used their recently developed Tn-seq method to


generate a detailed genotype–phenotype map of the pathogen _Streptococcus pneumoniae_. They measured the fitness of mutant libraries in 17 different _in vitro_ conditions and two _in vivo_


environments in mice. Using this approach, they uncovered leads for gene function and antibiotic action, and correlated _in vitro_ stress conditions with _in vivo_ colonization and disease


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T. et al. A fine scale phenotype-genotype virulence map of a bacterial pathogen. _Genome Res._ 23 Jul 2012 (doi:10.1101/gr.137430.112) Download references RIGHTS AND PERMISSIONS Reprints


and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Connecting genotypes and phenotypes. _Nat Rev Microbiol_ 10, 595 (2012). https://doi.org/10.1038/nrmicro2869 Download citation *


Published: 14 August 2012 * Issue Date: September 2012 * DOI: https://doi.org/10.1038/nrmicro2869 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this


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