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ABSTRACT Water-soluble and water-insoluble polysaccharide fractions were isolated from fruiting bodies of _Ganoderma lucidum_ (rheishi) by extraction with phosphate buffer and 1 N sodium

hydroxide solutions at different temperatures and investigated by high-performance liquid chromatography (HPLC), one- and two-dimensional 1H and 13C NMR, infrared (IR) spectroscopy, and

other techniques. This work was prompted by Sone _et al._’s earlier conclusion that similarly extracted, water-insoluble polysaccharides are (1→3)-β-D-glucans with very low degrees of

branching though hardly hydrolyzed by the enzyme _exo_-(1→3)-β-D-glucanase of _Basidiomycete_ QM 806. HPLC on the acid hydrolysates from our extracts showed that the water-soluble fractions

were minor constituents and mostly heteropolysaccharides, while the water-insoluble ones (_i.e._, the alkali-extracts at 25 and 65°C) were essentially pure glucans. It was found from NMR and

viscosity measurements in dimethylsulfoxide containing 0.25 M LiCl that these glucans consist of linearly linked (1→3)-α-D-glucose residues and differ only in molecular weight. The

(1→3)-α-structure of the water-insoluble fractions was confirmed by IR and optical rotation and also by comparison with the NMR spectra taken for standard (1→3)-β-D-glucan and

(1→3)-α-D-glucan samples. SIMILAR CONTENT BEING VIEWED BY OTHERS MOLECULAR CHARACTERIZATION OF A GALACTOMANNAN EXTRACTED FROM TARA (_CAESALPINIA SPINOSA_) SEEDS Article Open access 11

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Sarko, _Int. J. Biol. Macromol._, 3, 31 (1981). * J. Jelsma and D. R. Kreger, _Carbohydr. Res._, 71, 51 (1979). Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department

of Chemistry, Wuhan University, Jinghua Chen, Jinping Zhou & Lina Zhang * Department of Macromolecular Science, Osaka University, Yo Nakamura & Takashi Norisuye Authors * Jinghua

Chen View author publications You can also search for this author inPubMed Google Scholar * Jinping Zhou View author publications You can also search for this author inPubMed Google Scholar

* Lina Zhang View author publications You can also search for this author inPubMed Google Scholar * Yo Nakamura View author publications You can also search for this author inPubMed Google

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ARTICLE Chen, J., Zhou, J., Zhang, L. _et al._ Chemical Structure of the Water-Insoluble Polysaccharide Isolated from the Fruiting Body of _Ganoderma lucidum_. _Polym J_ 30, 838–842 (1998).

https://doi.org/10.1295/polymj.30.838 Download citation * Issue Date: 01 October 1998 * DOI: https://doi.org/10.1295/polymj.30.838 SHARE THIS ARTICLE Anyone you share the following link with

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content-sharing initiative KEYWORDS * Polysaccharide * _Ganoderma lucidum_ (Rheishi) * Chemical Structure * Nuclear Magnetic Resonance * (1→3)-α-D-Glucan * (1→3)-β-D-Glucan