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ABSTRACT Antigenicity and immunogenicity are important considerations in the laboratory evaluation of candidate varicella-zoster virus (VZV) vaccines. Therefore, high titered antisera were

prepared in guinea pigs and rabbits against two VZV strains: VZV-32 (a laboratory strain) and VZV-Oka (a vaccine strain). Radiolabeled proteins and glycoproteins were precipitated from

VZV-infected cells with the VZV immune sera, as well as human zoster sera, and analyzed by polyacrylamide gel electrophoresis. Three major glycopeptides of approximate mol. wt. 62,000,

98,000, and 118,000 were identified; two of the three glycoproteins have been found in the membranes of VZV-infected cells and in culture medium overlying VZV-infected cells. At least seven

nonglycosylated antigens, which ranged in mol. wt. from ∼30,000 to 150,000, also were present in the precipitates. The prominent high mol. wt. antigen probably corresponds to the major

capsid polypeptide found in herpesviruses. In summary, at least 10 polypeptides were consistently precipitated by VZV anti-sera obtained from hyperimmunized animals. Since both strains

elicited the same spectra of VZV antibodies, they could not be distinguished from one another on the basis of their electrophoretic profiles. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * Univ. Texas Hlth, Sci. Ctr., Dept. of Pediatrics, San Antonio, Texas Charles Grose Authors * Charles Grose View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Grose, C. 1020 THE ANTIGENIC DETERMINANTS OF VARICELLAZOSTER VIRUS. _Pediatr Res_

15 (Suppl 4), 612 (1981). https://doi.org/10.1203/00006450-198104001-01046 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01046 SHARE THIS

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