Reader TTS

ABSTRACT Chronic uremia is often associated with impaired utilization of glucose, presumably intracellularly, since it is not dependent on insulin or insulin redeptors. Using the circulating

polymophonuclear leukocyte (PMNL) as a cell model, 9 nondialyzed & 26 stable, maintenance hemodialyzed adults with chronic uremia (residual Ccr < 5ml/min) had significantly reduced

levels of glycolytic enzyme activities pyruvate kinase (PK) & glucose -6-PO4 dehydrogenese (G6PDH), ATP, & adenylate kinase (AK), protein (3 H Leu) & RNA (3H-URI) synthesis,

compared to 32 normal control subjects (blood donors). Following in vitro incubation of isolated PMNL in Krebs-Henseleit medium with 5mM glucose, cell levels of the glycolytic metabolites

G6P, F6P, & F-1-6-P were reduced, while DHAP, 3-PGA & PEP were increased, indicating a perturbation in glycolytic flux (GF), although lactate production was not impaired.

Hemodialysis improved AK activity & protein synthesis (PS), but G6PDH & ATP decreased. Lactate production by incubated PMNL fell. The postdialysis changes reverted to predialysis

values within 1 week. Stable maintenance hemodialysis patients got 500ml amino acid infusions (AAI) (Aminosyn 10%) at the end of each 3×/week dialysis. After 12 infusions (1 month),

predialysis ATP levels & PS reached normal levels. GF lactate production was unaffected but triose phosphate levels increased. To the extent that PMNL s reflect cell metabolism, the data

is consistent with a cellular defect in glycolysis & protein synthesis in chronic uremics. Dialysis, coupled with AAI, improves cell functions. ARTICLE PDF AUTHOR INFORMATION AUTHORS

AND AFFILIATIONS * University of Oklahoma Health Sciences Center & Veterans Administration Hospital, Oklahoma City, Oklahoma Jack Metcoff, Paul Costiloe, James Pederson, Billy Matter, 

Seshaehalam Dutta & Owen Rennert Authors * Jack Metcoff View author publications You can also search for this author inPubMed Google Scholar * Paul Costiloe View author publications You

can also search for this author inPubMed Google Scholar * James Pederson View author publications You can also search for this author inPubMed Google Scholar * Billy Matter View author

publications You can also search for this author inPubMed Google Scholar * Seshaehalam Dutta View author publications You can also search for this author inPubMed Google Scholar * Owen

Rennert View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Metcoff, J.,

Costiloe, P., Pederson, J. _et al._ 1525 CELL FUNCTIONS IN UREMIA IMPROVED BY DIALYSIS AND AMINO ACID INFUSIONS. _Pediatr Res_ 15 (Suppl 4), 697 (1981).

https://doi.org/10.1203/00006450-198104001-01548 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01548 SHARE THIS ARTICLE Anyone you share the

following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer

Nature SharedIt content-sharing initiative