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ABSTRACT This method requires the sequential addition of 5 μl increments of bilirubin solution (BS) of 25 mg/dl to 50 μl of neonatal serum (NS) in 2 ml of 200 mM phosphate buffer, pH 7.4.

With each addition of BS to the NS, there is a constant increase in absorbance at 484 nm until all PABS are exceeded. The table shows the expected absorbance change (Δ A484 nm) per addition

of BS which results in a linear relationship between added BS and Δ A484 nm until saturation of PABS. Deviation from linearity occurs when the saturation of PABS is exceeded. The volume of

BS that can be added to a NS before this point is reached provides an index of how far a patient is from the point at which bilirubin (BR) concentration will exceed primary capacity and

unbound BR will rapidly rise. The proposed method is less cumbersome than the methods currently being used, other than the less available BR hematofluorometer (Pediatrics 65:767, 1980).

Uniform lots of stable BS were provided by A. Louderback of Clinical Chemistry Consultants, Temple City, California. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Depts. of Path.

and Ped., Iowa Methodist Medical Center, Des Moines, IA Larry H Bernstein, Raul C Banagale, David Kingsley & A P Erenberg * Dept. of Ped., Univ. of Iowa Hospitals and Clinics, Iowa

City, IA. Larry H Bernstein, Raul C Banagale, David Kingsley & A P Erenberg Authors * Larry H Bernstein View author publications You can also search for this author inPubMed Google

Scholar * Raul C Banagale View author publications You can also search for this author inPubMed Google Scholar * David Kingsley View author publications You can also search for this author

inPubMed Google Scholar * A P Erenberg View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE

CITE THIS ARTICLE Bernstein, L., Banagale, R., Kingsley, D. _et al._ 225 RESERVE BILIRUBIN BINDING CAPACITY (RBBC) MEASURED BY ABSORBANCE DEVIATION (AD) AT SATURATION OF PRIMARY

ALBUMIN-BINDING SITES (PABS). _Pediatr Res_ 15 (Suppl 4), 477 (1981). https://doi.org/10.1203/00006450-198104001-00234 Download citation * Issue Date: 01 April 1981 * DOI:

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