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ABSTRACT Summary: Human milk samples were assayed for bile-salt-stimulated lipolytic activity in the presence of an unpurified and purified preparation of glycoconjugated bile salts and two

patterns of activity were obtained. One group of milks (high activity) had similar activities (mean ± 1 S.D.) of 16.9 ± 3.5 (purified bile salts) and 17.2 ± 3.5 μ mole·ml−1·h−1 (unpurified

bile salts) whereas another group (low activity) had a lower activity with the purified (4.2 ± 2.3 μ mole·ml−1·h−1) than with the unpurified bile salt preparation (19.9 ± 4.0 μ

mole·ml−1·h−1). Activities of the low activity milks (assayed with the purified bile salts) could be increased following storage at −20°C, sonication, or the preparation of an acetone ether

powder and, in some cases, reached the maximal activity obtained with the unpurified bile salt preparation. These procedures had no effect on the activity of the high activity milks.

Recombination experiments, whereby the milk serum from a low activity milk was added to the milk fat of a high activity milk and _vice versa_, and also kinetic studies suggest that the

differences in activities between the two groups result from differences in availability of the enzyme and are independent of the source of milk fat. SIMILAR CONTENT BEING VIEWED BY OTHERS

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ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Child Health, Institute of Child Health, 30 Guilford Street, WCIN IEN, London, England Barbara Hall & D P R Muller

Authors * Barbara Hall View author publications You can also search for this author inPubMed Google Scholar * D P R Muller View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hall, B., Muller, D. Studies on Bile-Salt-Stimulated Lipolytic Activity in Human

Milk. II Demonstration of Two Groups of Milk with Different Activities. _Pediatr Res_ 17, 716–720 (1983). https://doi.org/10.1203/00006450-198309000-00006 Download citation * Issue Date: 01

September 1983 * DOI: https://doi.org/10.1203/00006450-198309000-00006 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link

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