Reader TTS

ABSTRACT We examined nasal secretions (NS) of 13 patients (pts) age 1-8 months (mos) hospitalized with acute RSV infection for IgA Ab to the surface glycoproteins (66K and 84K) and

nucleocapsid protein (NCP) of RSV. Acute and convalescent (conv) NS (obtained 3-17 days (d) after initial NS) were examined by enzyme-linked immunosorbent assay (ELISA) using individual

mouse monoclonal Abs (RSV Long strain) to 66K, 84K, and NCP and RSV hyperimmune horse serum (HARS) as capture Abs. Competition assays demonstrated that the antigens captured in this system

were specific for each monoclonal Ab used. Total IgA Ab to RSV measured by HARS showed a ≥ 3-fold rise in Ab titer in 9/13 pts. Ab rise (≥ 3-fold) to 66K (fusion) glycoprotein occurred in

5/13 pts; 84K, 3/13 pts; and NCP, 6/13 pts. Ab rise was most striking in the 7 pts with later conv NS (≥ 6 d after acute NS). All but the youngest (1 mo) of these 7 pts had a ≥ 3-fold rise

in total RSV Ab measured by HARS capture. Three, 2, and 4 of these pts had Ab rises to the 66K, 84K, and NCP respectively. Only 2/13 pts, both aged 1 mo, had no detectable RSV-specific Ab in

NS. Ab rise in the remaining pts could not be explained by differences in IgA content between acute and conv NS. We conclude that monoclonal Ab capture ELISA can be used to detect

class-specific Ab in infant NS and that infants recovering from RSV make IgA Ab to NCP and both surface glycoproteins. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *

Children's Hospital, Div. Inf. Dis., Boston, MA. J C Burns, R M Hendry, J C Ho, B Fernie & K McIntosh * Georgetown University, Washington, D.C. J C Burns, R M Hendry, J C Ho, B

Fernie & K McIntosh Authors * J C Burns View author publications You can also search for this author inPubMed Google Scholar * R M Hendry View author publications You can also search for

this author inPubMed Google Scholar * J C Ho View author publications You can also search for this author inPubMed Google Scholar * B Fernie View author publications You can also search for

this author inPubMed Google Scholar * K McIntosh View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT

THIS ARTICLE CITE THIS ARTICLE Burns, J., Hendry, R., Ho, J. _et al._ ANTIGEN-SPECIFIC SECRETORY ANTIBODY (Ab) RESPONSE FOLLOWING RESPIRATORY SYNCYTIAL VIRUS (RSV) INFECTION IN INFANTS.

_Pediatr Res_ 18 (Suppl 4), 270 (1984). https://doi.org/10.1203/00006450-198404001-01065 Download citation * Issue Date: 01 April 1984 * DOI: https://doi.org/10.1203/00006450-198404001-01065

SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy

to clipboard Provided by the Springer Nature SharedIt content-sharing initiative