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ABSTRACT We have developed a method for demonstration and quantitation of the anionic charge sites in human alveolar-capillary basement membrane (ABM). Fixed-frozen sections of normal lung

were incubated with the cationic polymer polyethylenimine (PEI) and processed for electron microscopy using methods recently described for kidney (NEJM 309:1001, 1983). Results: The number

of anionic sites in the lamina rara externa (LRE) of the AMB is remarkably consistent in multiple random samples of normal lung of differing ages and is not influenced by the molecular

weight of the probe. Smaller numbers of sites are also present in the lamina rara interna and on cell membranes. Alteration of anionic charges in the ABM may influence permeability of

negatively-charged protein across the membrane. This hypothesis can now be assessed in man by this method. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pediatrics,

University of Minnesota Medical School, Minneapolis, MN Bruce Ferrara, Susan Sisson & Robert L Vernier Authors * Bruce Ferrara View author publications You can also search for this

author inPubMed Google Scholar * Susan Sisson View author publications You can also search for this author inPubMed Google Scholar * Robert L Vernier View author publications You can also

search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Ferrara, B., Sisson, S. & Vernier, R. ANIONIC CHARGE

SITES IN NEONATAL ALVEOLAR CAPILLARY BASEMENT MEMBRANES. _Pediatr Res_ 18 (Suppl 4), 391 (1984). https://doi.org/10.1203/00006450-198404001-01786 Download citation * Issue Date: 01 April

1984 * DOI: https://doi.org/10.1203/00006450-198404001-01786 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a

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