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The role of agonist and constitutive nitric oxide (NO) release in the regulation of human placental vascular tone are not known. ADM is a recently discovered hypotensive peptide isolated

from pheochromocytoma cells that shares structural homology to Calcitonin Gene Related Peptide, (CGRP). Adrenomedullin has been shown to produce vascular relaxation through NO and cyclic AMP

mediated intracellular signal pathways. We investigated in vitro responses to both peptides in cannulated chorionic plate arteries (CPA) from term uncomplicated pregnancies. Vessels were

cannulated on micropipettes under constant intraluminal pressure and changes in diameter (<400 microns) were measured through a videodimension analyzer. Percent relaxation in response to

0.1uM synthetic human ADM and CGRP were observed following submaximal preconstriction with U46619. Vessels were rechallenged with either peptide following equilibration with 0.1mM of the NO

synthase inhibitor L-NNA. An additional group was studied following removal of the endothelium (EC-) by perfusing the lumen with lcc air at atmospheric pressure. Results shown below are Mean

± SEM (n=6) percent increase lumen diameter (*, p<0.05 vs. control). Membrane receptors and intracellular signal pathways to both peptides are present in term CPA. The in vitro response

to ADM is mediated by endothelial NO release. Selective inhibition of the response to ADM but not CGRP by L-NNA strongly suggests two distinct receptor populations. Constitutive NO release

may play an important role in the regulation of placental vascular tone in vivo. Table AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Dept. of Pediatrics, Tulane University School of

Medicine, New Orleans LA., Robert L Pierce, Maria R Pierce, Haiyan Liu & Arthur S Pickoff Authors * Robert L Pierce View author publications You can also search for this author inPubMed 

Google Scholar * Maria R Pierce View author publications You can also search for this author inPubMed Google Scholar * Haiyan Liu View author publications You can also search for this author

inPubMed Google Scholar * Arthur S Pickoff View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS

ARTICLE CITE THIS ARTICLE Pierce, R., Pierce, M., Liu, H. _et al._ ADRENOMEDULLIN MEDIATED NITRIC OXIDE RELEASE IN THE REGULATION PLACENTAL ARTERIAL VASCULAR TONE. • 451. _Pediatr Res_ 39

(Suppl 4), 78 (1996). https://doi.org/10.1203/00006450-199604001-00471 Download citation * Issue Date: 01 April 1996 * DOI: https://doi.org/10.1203/00006450-199604001-00471 SHARE THIS

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