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ABSTRACT Three new compounds including two thiodiketopiperazine derivatives shovelmycins A–B (1–2), and one ansamycin derivative divergolide X (3) were isolated and identified from the

culture extract of _Streptomyces olivaceus_ HDN22-001, a marine actinomycete obtained from the deep-sea cold seep sediment sample collected from the South China Sea. Their structures and

absolute configurations were determined by spectroscopic analyses and ECD calculations. Compound 1 exhibited the strongest DPPH radical scavenging activity with an IC50 value of 10.83 μM,

which was better than that of the positive control vitamin C. And compound 2 was modestly cytotoxic against NCl-H446 cell with the IC50 value of 26.6 μM. Access through your institution Buy

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OTHERS A NEW STEROID WITH POTENT ANTIMICROBIAL ACTIVITIES AND TWO NEW POLYKETIDES FROM _PENICILLIUM VARIABILE_ EN-394, A FUNGUS OBTAINED FROM THE MARINE RED ALGA _RHODOMELA CONFERVOIDES_

Article 26 October 2023 ANTIBACTERIAL _P_-TERPHENYL AND _Α_‑PYRONE DERIVATES ISOLATED FROM THE MARINE-DERIVED ACTINOMYCETE _NOCARDIOPSIS_ SP. HDN154086 Article 25 January 2024 RAUSUQUINONE,

A NON-GLYCOSYLATED PLURAMYCIN-CLASS ANTIBIOTIC FROM _RHODOCOCCUS_ Article 30 November 2021 REFERENCES * Cong M, Pang X, Zhao K, Song Y, Liu Y, Wang J. Deep-sea natural products from extreme

environments: cold seeps and hydrothermal vents. Mar Drugs. 2022;20:404. CAS  PubMed  PubMed Central  Google Scholar  * Rampelotto PH. Extremophiles and extreme environments. Life.

2013;3:482–5. PubMed  PubMed Central  Google Scholar  * Brown PD, Lawrence AL. The importance of asking “how and why?” in natural product structure elucidation. Nat Prod Rep.

2017;34:1193–202. CAS  PubMed  Google Scholar  * Dubilier N, Bergin C, Lott C. Symbiotic diversity in marine animals: the art of harnessing chemosynthesis. Nat Rev Microbiol. 2008;6:725–40.

CAS  PubMed  Google Scholar  * Levin LA. Ecology of cold seep sediments: interactions of fauna with flow, chemistry and microbes. Oceanogr Mar Biol. 2005;43:1–46. Google Scholar  * Chi LP,

Li XM, Wan YP, Li X, Wang BG. Ophiobolin sesterterpenoids and farnesylated phthalide derivatives from the deep sea cold-seep-derived fungus _Aspergillus insuetus_ SD-512. J Nat Prod.

2020;83:3652–60. CAS  PubMed  Google Scholar  * Choi BK, Trinh PTH, Lee HS, Choi BW, Kang JS, Ngoc NTD, et al. New ophiobolin derivatives from the marine fungus _Aspergillus flocculosus_ and

their cytotoxicities against cancer cells. Mar Drugs. 2019;17:346. CAS  PubMed  PubMed Central  Google Scholar  * Li X, Li L, Li XM, Li HL, Konuklugil B, Wang BG. Ustusaustin A: a new

neuraminidase inhibitory meroterpene from the ascidian-derived endophytic fungus _Aspergillus ustus_ TK-5. Nat Prod Res. 2021;35:4939–44. CAS  PubMed  Google Scholar  * Che YH, Wang JF, Ding

WP, Xiao ZH, Shi XF, Wu JM, et al. New diketopiperazine alkaloids from the Haima cold seep-derived fungus _Toxicocladosporium_ sp. CYH-18. Phytochem Lett. 2024;60:96–100. CAS  Google

Scholar  * Hu XY, Wang CY, Li XM, Yang SQ, Li X, Wang BG, et al. Cytochalasin derivatives from the endozoic _Curvularia verruculosa_ CS-129, a fungus isolated from the deep-sea squat lobster

_Shinkaia crosnieri_ living in the cold seep environment. J Nat Prod. 2021;84:3122–30. CAS  PubMed  Google Scholar  * Ying Z, Li XM, Yang SQ, Li HL, Li X, Wang BG, et al. Pseudallenes A and

B, new sulfur-containing ovalicin sesquiterpenoid derivatives with antimicrobial activity from the deep-sea cold seep sediment-derived fungus _Pseudallescheria boydii_ CS-793. Beilstein J

Org Chem. 2024;20:470–8. CAS  PubMed  PubMed Central  Google Scholar  * Dong Y, Wang JingE, Bao R, Li Y. H. New Olimycins from a Cold‐Seep‐Derived _Streptomyces olivaceus_. Chem Biodivers.

2023;20:e202300689. CAS  PubMed  Google Scholar  * Jin E, Li H, Liu Z, Xiao F. Antibiotic Dixiamycins from a Cold-Seep-Derived _Streptomyces olivaceus_. J Nat Prod. 2021;84:2606–11. CAS 

PubMed  Google Scholar  * Chen CL, Hu SJ, Zhu LL, Yao LM, Wang XX, Zhang AN, et al. Isolation and identification of chemical constituents of _Rubi Fructus_, screening novel cannabinoid CB2

receptor agonist and evaluation of its anti-osteoporosis effect. Chin Tradit Herbal Drugs. 2024;55:386–401. Google Scholar  * Furukawa T, Akutagawa T, Funatani H, Uchida T, Hotta Y, Niwa M,

et al. Cyclic dipeptides exhibit potency for scavenging radicals. Bioorg Med Chem. 2012;20:2002–9. CAS  PubMed  Google Scholar  * Zhao G, Li S, Guo Z, Sun M, Lu C. Overexpression of div 8

increases the production and diversity of divergolides in _Streptomyces_ sp. W112. RSC adv. 2015;5:98209–14. CAS  Google Scholar  * Tu L, Shen S, Yan Z, Li X, Liu K, Xu J, et al. Discovery

of olimycin E from _Streptomyces_ sp. 11695. Nat Prod Res. 2024;4:1–6. Google Scholar  * Frisch MJ, Trucks GW, Schlegel HB, Scuseria GE, Robb MA, Cheeseman JR, et al. Gaussian 09, Revision

A.1, Gaussian Inc. Wallingford CT;USA;2009. * Bruhn T, Schaumlöffel A, Hemberger Y, Bringmann G SpecDis, Version 1.53. University of Wuerzburg;Germany;2011. * Sharma OP, Tej KB. DPPH

antioxidant assay revisited. Food Chem. 2009;113:1202–5. CAS  Google Scholar  * Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother. 2001;48:5–16. CAS 

PubMed  Google Scholar  * Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55–63. CAS 

PubMed  Google Scholar  * Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst.

1990;82:1107–12. CAS  PubMed  Google Scholar  Download references ACKNOWLEDGEMENTS This work was supported by the Fundamental Research Funds for the Central Universities (202362008,

202172002), the Shandong Provincial Natural Science Foundation (ZR2021MH257), the National Key Research and Development Program of China (2022YFC2804400). AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * School of Medicine and Pharmacy, Key Laboratory of Marine Drugs Ministry of Education, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University

of China, Qingdao, People’s Republic of China Chen Li, Jiangli Cheng, Ruojin Liu, Xiaofei Huang, Luning Zhou, Guojian Zhang, Tianjiao Zhu, Dehai Li & Qian Che * Laboratory for Marine

Drugs and Bioproducts, Laoshan Laboratory, Qingdao, People’s Republic of China Guojian Zhang, Tianjiao Zhu, Dehai Li & Qian Che Authors * Chen Li View author publications You can also

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agreement and applicable law. Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Li, C., Cheng, J., Liu, R. _et al._ The thiodiketopiperazine derivatives shovelmycin A–B and

ansamycin derivative divergolide X from the cold-seep-derived _Streptomyces olivaceus_ HDN22-001. _J Antibiot_ 78, 275–280 (2025). https://doi.org/10.1038/s41429-025-00812-z Download

citation * Received: 28 November 2024 * Revised: 23 January 2025 * Accepted: 16 February 2025 * Published: 04 March 2025 * Issue Date: April 2025 * DOI:

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