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The lack of endogenous reporter lines is a bottleneck in the study of subcellular dynamics in human adult stem cell (ASC)-derived organoids. An approach using CRISPR–Cas9-mediated

homology-independent organoid transgenesis (CRISPR–HOT) in ASC-derived organoids now narrows the gap between basic research and translational studies in human organoids. Access through your

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during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Lancaster, M. A. & Huch, M. _Dis.

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equally: Qiutan Yang, Koen C. Oost. AUTHORS AND AFFILIATIONS * Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland Qiutan Yang, Koen C. Oost & Prisca Liberali

Authors * Qiutan Yang View author publications You can also search for this author inPubMed Google Scholar * Koen C. Oost View author publications You can also search for this author

inPubMed Google Scholar * Prisca Liberali View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Prisca Liberali. ETHICS

DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yang, Q., Oost, K.C. &

Liberali, P. Engineering human knock-in organoids. _Nat Cell Biol_ 22, 261–263 (2020). https://doi.org/10.1038/s41556-020-0478-z Download citation * Published: 02 March 2020 * Issue Date:

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