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Access through your institution Buy or subscribe Grosselin, K. et al. _Nat. Genet_. 51, 1060–1066 (2019). Rare cell types can have an outsized biological effect, but their sparsity makes

them impossible to detect in bulk samples. To study the effect of such heterogeneity, Grosselin et al. developed a microfluidic device for single-cell chromatin immunoprecipitations. In one

channel, cells are embedded in droplets, lysed and treated with MNase to fragment their chromatin; in another channel, barcoded beads are encapsulated. Following fusion of these two types of

droplets, the nucleosomes in each cell are tagged with a specific barcode. After pooling and immunoprecipitation of activating and repressing histone marks, each modification can be traced

back to its cell of origin. The authors probed hundreds of cells from drug-resistant and susceptible breast tumors, and found a resistance signature in a low percentage of drug-sensitive

cells. At 4% of the overall population, this cell type was too sparse to be detected in a bulk sample, but it can provide important clues about the development of drug resistance during

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ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Nature Methods

http://www.nature.com/nmeth Nicole Rusk Authors * Nicole Rusk View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to

Nicole Rusk. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Rusk, N. Single-cell ChIP. _Nat Methods_ 16, 680 (2019).

https://doi.org/10.1038/s41592-019-0523-7 Download citation * Published: 30 July 2019 * Issue Date: August 2019 * DOI: https://doi.org/10.1038/s41592-019-0523-7 SHARE THIS ARTICLE Anyone you

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