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One major challenge in neuroscience is to gain a systematic understanding of the extraordinary diversity of brain cell types and how they contribute to brain function. Spatially resolved

transcriptomics holds unmatched promise in unraveling the organization of brain cell types and their relationship with connectivity, circuit dynamics, behavior and disease. Here we discuss

neuroscience applications of various spatially resolved transcriptomics methods, as well as technical challenges that need to be overcome to realize their full potentials. Access through

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calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Zeng, H. & Sanes, J. R.

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references ACKNOWLEDGEMENTS This work was supported by Allen Institute for Brain Science and by grant U19MH114830 from the National Institute of Mental Health to H.Z. The content is solely

the responsibility of the authors and does not necessarily represent the official views of NIH and its subsidiary institutes. The authors wish to thank the Allen Institute founder, Paul G.

Allen, for his vision, encouragement and support. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Allen Institute for Brain Science, Seattle, WA, USA Jennie L. Close, Brian R. Long & 

Hongkui Zeng Authors * Jennie L. Close View author publications You can also search for this author inPubMed Google Scholar * Brian R. Long View author publications You can also search for

this author inPubMed Google Scholar * Hongkui Zeng View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Hongkui Zeng.

ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Close, J.L., Long,

B.R. & Zeng, H. Spatially resolved transcriptomics in neuroscience. _Nat Methods_ 18, 23–25 (2021). https://doi.org/10.1038/s41592-020-01040-z Download citation * Published: 06 January

2021 * Issue Date: January 2021 * DOI: https://doi.org/10.1038/s41592-020-01040-z SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

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