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ABSTRACT We report noncovalent assemblies of iRGD peptides and methylene blue dyes via electrostatic and hydrophobic stacking. These resulting nanomaterials could bind to cancer cells, image

them with photoacoustic signal, and then treat them via photodynamic therapy. We first assessed the optical properties and physical properties of the materials. We then evaluated their

utility for live cell targeting, in vivo imaging, and in vivo photodynamic toxicity. We tuned the performance of iRGD by adding aspartic acid (DD) or tryptophan doublets (WW) to the peptide

to promote electrostatic or hydrophobic stacking with methylene blue, respectively. The iRGD-DD led to 150-nm branched nanoparticles, but iRGD-WW produced 200-nm nano spheres. The branched

particles had an absorbance peak that was redshifted to 720 nm suitable for photoacoustic signal. The nanospheres had a peak at 680 nm similar to monomeric methylene blue. Upon continuous

irradiation, the nanospheres and branched nanoparticles led to a 116.62% and 94.82% increase in reactive oxygen species in SKOV-3 cells relative to free methylene blue at isomolar

concentrations suggesting photodynamic toxicity. Targeted uptake was validated via competitive inhibition. Finally, we used in vivo bioluminescent signal to monitor tumor burden and the

effect of for photodynamic therapy: The nanospheres had little impact versus controls (_p_ = 0.089), but the branched nanoparticles slowed SKOV-3 tumor burden by 75.9% (_p_ < 0.05).

SIMILAR CONTENT BEING VIEWED BY OTHERS BIOINSPIRED CARRIER-FREE PEPTIDE CONJUGATED BF2-OXASMARAGDYRIN DYE-BASED NANO SELF-ASSEMBLIES: A PHOTOSTABLE NIR CANCER THERAGNOSTIC AGENT Article Open

access 04 December 2020 NANOPARTICLES DESTABILIZING THE CELL MEMBRANES TRIGGERED BY NIR LIGHT FOR CANCER IMAGING AND PHOTO-IMMUNOTHERAPY Article Open access 17 July 2024 A SUPRAMOLECULAR

PHOTOSENSITIZER DERIVED FROM AN ARENE-RU(II) COMPLEX SELF-ASSEMBLY FOR NIR ACTIVATED PHOTODYNAMIC AND PHOTOTHERMAL THERAPY Article Open access 02 June 2022 INTRODUCTION New cancer therapies

are urgently needed, and targeted therapies have shown value in reducing off-target effects while increasing therapeutic efficacy. Peptide-based agents have shown specific value when

engineered to interact with T-cell epitopes for triggered immunogenicity1, upregulated membrane receptors in solid tumors2,3,4, and enzyme biomarkers for targeted inhibition. Peptides also

have value when used to direct a therapeutic or diagnostic agent to the site of disease, but covalent ligation between peptides and a therapeutic or diagnostic molecular agent can suffer

from modest conjugation yields, thus bottlenecking scale-up5,6. Self-assembling peptide nanoparticles (SAPNs) are an important alternative to covalent chemistry when linking peptides and

cargo. Here, peptide sequences are engineered so that one domain has biologically activity, i.e., targeting, while another domain tunes the peptide’s physical properties via zwitterionic,

charged, or hydrophobic regimes7,8. Examples include tissue regeneration, targeted cytotoxicity, nucleic acid delivery, activatable immunotherapy9, and molecular imaging10,11. One limitation

of SAPNs is that synthesis usually yields nanoparticles with various architectures and shapes, which can affect their interactions with biology. While many reports discuss the ideal

application for different resultant geometries of SAPNs12, few reports empirically compare the performance of different geometries in parallel. Here, we investigate non-covalent interactions

between a tumor-homing peptide motif (iRGD) and a molecular dye (methylene blue). iRGD was chosen as the model peptide because it is a well-established and robust bimodal targeting peptide

against solid tumors: iRGD first internalizes into cancer cells via αVβ3 and αVβ5 integrin recognition13. Upon cleavage into CRGDK/R, the exposed C-endR motif (R/KXXR/K) allows the

fragmented peptide to enable the tumor microenvironment (TME) vasculature permeability through neuropilin-1 (NRP-1) interactions14. This peptide offers active targeted drug delivery by mere

co-loading the peptide and the drug rather than covalent tethering15. The photodynamic therapeutic used here is methylene blue—an FDA-approved dye and longstanding workhorse in the clinical

landscape; it was selected as a model molecular dye here because it is both hydrophobic and cationic, thus making it possible to explore two possible mechanisms of self-assembly. Methylene

blue also absorbs light in the NIR window (~670 nm) suitable for imaging16. Its redox potential also enables activatable reactive oxygen species (ROS) generation for therapy (Fig. 1)17. We

combined the methylene blue and iRGD peptide into SAPNs to make a hybrid construct that offers targeting, therapy, and imaging. The approach used intermolecular forces rather than covalent

bonds. Here, the iRGD molecule was modified with additional amino acids on the C-terminus—either aspartic acid or tryptophan. Aspartic acid (D) facilitates electrostatic interactions between

iRGD and methylene blue, while tryptophan (W) produces hydrophobic stacking. These different intermolecular forces lead to different nanoparticle morphologies. After characterizing the

materials’ physical properties, we then studied the value of these materials in imaging tumors with photoacoustic imaging and treating tumors with photodynamic therapy. The results show that

the SAPN morphology is controllable via peptide engineering and that the product can markedly reduce tumor growth. The product offers targeting via the iRGD motif, photoacoustic imaging via

the methylene blue cargo, and therapy via photodynamic therapy of the methylene blue cargo. RESULTS PEPTIDE DESIGN AND ASSEMBLY WITH METHYLENE BLUE Native and designer iRGD peptides were

synthesized using solid phase synthesis. iRGD (CRGDKGPDC) was tagged with either aspartic acid doublets (iRGD-DD) or tryptophan doublets (iRGD-WW): We hypothesized that the negatively

charged aspartic acids would facilitate electrostatic assemblies between the peptides and methylene blue; the tryptophan groups would form hydrophobic assemblies. The products were purified

via reverse phase high performance liquid chromatography (RP-HPLC) and characterized via ESI-mass spectrometry (Fig. S1). Although native iRGD adopts a cyclic structure from disulfide

bridging between cysteines, computational simulations demonstrated that the designer peptides do not adopt a secondary structure (Fig. S2). Multispectral advanced nanoparticle tracking

analysis (MANTA) was used to monitor size changes and nanoparticle formations during assembly of peptide and methylene blue (Fig. 2A–C). First, native iRGD peptides were mixed with methylene

blue at a 10:1 molar ratio in water. MANTA demonstrated that these mixtures did not yield any nanoparticles: The maximum average size of 9 nm, which is similar to peaks seen with native

iRGD peptide in water alone (Fig. 2A). When iRGD-DD peptides were mixed with methylene blue at the same molar ratio in water, the size increased and stabilized to about 178 nm particles

within 90 min (Fig. 2B). This assembly was caused by electrostatic attraction between the aspartic acid doublets and the methylene blue. To demonstrate this, we repeated the same assembly

conditions but under pH conditions that surpassed the isoelectric points of either iRGD-DD or methylene blue. When the pH was set to 12, methylene blue adopted a neutral charge, which did

not result in stable nanoparticulate formations. The same results were observed when the pH was set below 2 where iRGD-DD has neutral charge. In both cases, the most abundantly observed

sizes from MANTA were below 10 nm (Fig. S3A–D). When iRGD-WW peptides were mixed with methylene blue at a similar 10:1 molar ratio in water, 150 nm nanoparticles were formed under the same

90-minute time frame (Fig. 2C). This assembly was likely due to hydrophobic stacking between the tryptophan doublets and the methylene blue molecules. To confirm this hypothesis, we studied

assembly in DMSO and chloroform, which disrupt hydrophobic interactions from tryptophan. Here, no nanoparticle assembly was observed (Fig. S3E–F). Thus, both iRGD-DD and iRGD-WW based

assemblies can produce SAPNs18. We used transmission electron microscopy (TEM) to image the products (Fig. 2D). The individual methylene blue molecules and peptides did not show any

noticeable particles. Similarly, a mixture between native iRGD and methylene blue did not reveal any visible assemblies besides some nonspecific aggregates. In contrast, the

iRGD-DD-methylene blue assemblies were seen as branched nanoparticles (“iRGD-BNPs”), while iRGD-WW-methylene blue assemblies were spherical (iRGD-SNPs) (see also Fig. S4). The sizes of these

particulates agreed with the MANTA measurements. We further studied the impact of different ratios of peptides to methylene blue using MANTA analysis (Fig. S5). The number of particles

produced was not significantly different than PBS background until there were two equivalents of peptide per MB for the iRGD-BNP and four equivalents of peptide per methylene blue for

iRGD-SNP. Thus, all work above and below used 10 equivalents of peptide because this concentration led to the maximum particle formation (Fig. S5). Finally, we tested the stability of the

products in different pH values including in physiological conditions. The results showed a slight increase at more acidic conditions typical of tumor environment (Fig. S6). The particles

were also stable for up to a month when refrigerated in the dark. LIVE CELL INTERACTIONS BETWEEN THE PEPTIDE-DYE ASSEMBLIES We next evaluated targeted photodynamic toxicity. SKOV-3 cells

were incubated with either methylene blue, a mixture of methylene blue with native iRGD, iRGD-BNPs, or iRGD-SNPs. The cells were then irradiated with a 100 mW 660 nm light to activate ROS

production from the methylene blue (Fig. 3a). ROS production was monitored with a fluorescent DCFDA assay. This experiment showed the iRGD-SNPs and iRGD-BNPs produced 116.6% and 94.82% more

ROS than SKOV3 cells treated with methylene blue alone, respectively (Fig. 3d, e). We also observed increased fluorescence from the DCFH-DA (153.7% and 134.2% increased fluorescence above

free methylene blue for the nanospheres and branched NPs respectively) for MCF-7 human breast cancer cells (Fig. 3d, e). ROS fluorescence was lower when cells were not irradiated with light.

To further corroborate that ROS production was due to light activation and photodynamic toxicity, we next designed an area-specific light experiment: A certain area of the cell culture

flask was irradiated with the NIR laser after treating with the iRGD-BNPs and iRGD-SNPs. ROS-based fluorescence was seen with fluorescent microscopy. There was more green fluorescence in the

laser treated area (Fig. S7). These data confirmed that light irradiation generated ROS from MB. Hoechst staining also showed that ROS generation resulted in increased apoptosis (Fig. S7).

Both iRGD-BNPs and iRGD-SNPs formulations were then incubated with cells to evaluate targeting capabilities. We performed a control with different cells lines with differing integrin

expression levels. (SKOV-3, MCF-7, and HEK 293T integrin expression levels were validated with flow cytometry experiments (Fig. S8)). The iRGD-BNPs, iRGD-SNPs, and methylene blue were

incubated with HEK 293T cells as a negative control (Fig. 3d, e). There was no elevated ROS fluorescence when the HEK 293T cells were incubated with the assemblies. The peptide formulations

were further challenged via competitive inhibition flow cytometry studies to confirm their specific interactions with integrin receptors (Fig. 3b, c). In addition to labeling as before, some

cells were pre-incubated with αVβ5 primary antibodies before iRGD-BNPs or iRGD-SNPs. Fluorescence from the cells SKOV-3 treated with αVβ5 antibodies prior to iRGD-BNPs or iRGD-SNPs was less

than the fluorescence detected when they were treated with free methylene blue alone. There was increased fluorescence when the cells were treated with iRGD-BNPs or iRGD-SNPs alone. There

was no observable change in fluorescence from the presence of the antibodies for HEK 293-T cells. These data confirmed that the iRGD peptide retained its αVβ5 -mediated uptake into cells.

Endosomal escape has remained as a longstanding issue for nanoparticle drug delivery19, and past reports have also validated that the iRGD peptide inherently increases endocytosis-mediated

uptake20. Therefore, it was important to examine if the iRGD nanospheres and branched NPs could escape the endosome. We characterized calcein leakage fluorescence by flow cytometry after

co-incubating the membrane-impermeable and pH sensitive calcein dye either with iRGD-BNPs, iRGD-SNPs, methylene blue, or mixtures of iRGD and methylene blue (Fig. 3f)21,22. The cells

incubated with the iRGD-BNPs showed 76.2% of the population was double positive in methylene blue and calcein fluorescence and 14.9% of the population was predominantly positive in methylene

blue fluorescence, while the iRGD-SNPs showed 61.6% of the population was double positive in methylene blue and calcein fluorescence while 32.2% of the population was predominantly positive

in methylene blue fluorescence. The results showed that while much of the methylene blue is internalized into the cells from the spherical formulation, it could not escape the endosome,

unlike branched nanoparticles. OPTICAL CHARACTERIZATION AND PHOTOACOUSTIC IMAGING WITH THE PEPTIDE DYE ASSEMBLIES The optical and photoacoustic properties of the assemblies were also studied

(Fig. 4). When the nanospheres and branched NPs were scanned from 400 to 700 nm for optical absorbance, both adopted a similar peak absorbance to methylene blue at 662 nm. When the peaks

were normalized, there was no 614 nm shoulder growth where dimerization typically forms23. While the iRGD-BNP maintained methylene blue’s distinct blue color, the iRGD-SNP samples shifted to

a slightly greener color. The quantum yield for the branched NPs and the nanospheres relative to methylene blue (52%) were 43% and 31%, respectively, because there was increased

fluorescence self-quenching between the assemblies relative to the methylene blue reference at the same optical density at the same wavelength (Fig. S9). The photoacoustic contrast of the

formulations was evaluated in vitro and in vivo. First, the samples were loaded into tubes, placed under water, and irradiated under a pulsed NIR laser and imaged with an ultrasound

transducer. Both the nanospheres and branched NPs had photoacoustic contrast at 680 nm, which is the native peak photoacoustic intensity of methylene blue. There was no significant

photoacoustic enhancement between the cases (_p_ = 0.051). However, the branched NPs maintained distinct photoacoustic contrast at 720 nm while the nanospheres, mixture between methylene

blue and iRGD, and methylene blue lost visibility (_p_ < 0.0001) (Fig. 4a, b). Even when the iRGD-DD to methylene blue molar ratio was increased, these assemblies maintained a 720 nm

photoacoustic contrast (Fig. S10). These results suggest that these non-covalent assemblies can maintain native optical properties or promote aggregation-induced shifts such as J aggregation

and self-quenching based on simply tuning the peptide sequence. Photoacoustic imaging has demonstrated ability to image diseased tissue with excellent contrast and with accessory

photodynamic properties24,25. For the in vivo experiments, mice were xenografted with SKOV-3 cells intraperitoneally. There were six groups: (1) SKOV-3 positive and imaged with free

methylene blue, (2) SKOV-3 positive and imaged with a mixture of iRGD and methylene blue, (3) SKOV-3 positive and imaged with the iRGD-BNPs, (4) SKOV-3 positive and imaged with the

iRGD-SNPs, (5) SKOV-3 negative and imaged with the iRGD-BNPs, and (6) SKOV-3 negative and imaged with the iRGD-SNPs. The mice were imaged 10 days after inoculation. When the nanospheres and

branched NPs were locally injected, PA contrast and intensity increased at a faster rate over the span of 15 min, while the PA contrast rate was slower when iRGD was co-injected with

methylene blue. PHOTODYNAMIC THERAPY AGAINST TUMOR BEARING MICE Targeted photodynamic therapy against tumor-bearing mice was also evaluated. Mice were inoculated with SKOV-3

intraperitoneally, but these cells expressed the luciferase gene for bioluminescence monitoring over time. Three days after inoculation, they were treated either with (1) PBS (2) methylene

blue (3) iRGD+methylene Blue, (4) iRGD-SNPs, or (5) iRGD-BNPs over the nine-day period. After overnight incubation, the subjects were exposed to a 100 mW of 660-nm light locally pointed at

the abdomen for 10 min each (Fig. 5a). Interestingly, the iRGD + methylene blue mixture and the iRGD-BNPs treatments yielded the slowest tumor growth rate, while the iRGD-NS treatment was

less efficient. In particular, the branched NPs decreased tumor burden bioluminescence by an average 75.91% (+/−11.48%) (_p_ = 0.043), while spheres decreased tumor burden bioluminescence by

an average of 58.89% (+/−18.23%) (_p_ = 0.089). There was no significant difference in the decreased bioluminescence between the branched NPs and the co-loaded iRGD and methylene blue

treatment (_p_ = 0.7941) (Fig. 5a–c). The vital organs of the subjects were harvested after euthanasia and analyzed for fluorescence from the methylene blue for biodistribution studies.

Here, the subjects treated with only methylene blue showed nonspecific and low fluorescence in all of the vital organs. The iRGD-SNPs-, iRGD-BNPs-, and iRGD+MB-treated subjects showed at

least 2-fold heightened fluorescence in the extracted tumor. The iRGD-SNPs showed over 5-fold increased fluorescence in the liver above the other two cases (Fig. 5d). The increased

fluorescence in the liver indicates preferential liver clearance of spherical nanoparticles above the branched NPs nanoparticles. Finally, the targeting capabilities for the peptides were

analyzed and compared in silico. In summary, iRGD-WW had a stronger predicted binding energy of an average docking score (affinity) of −9.82, compared to that (−8.22) of iRGD-DD to the αVβ3

receptor (Figs. S11–S14). In addition, the docking ligand interactions between the peptides and receptor were compared to the ligand interactions of the co-crystallized iRGD- αVβ3 complex

structure (PDB 1L5G). The predicted conformations of iRGD-WW were within similar residues to iRGD’s location on the protein surface, with direct ligand interactions with directly neighboring

residues to the putative binding residues of the iRGD motif (Figs. S11, S12, S14). The iRGD-DD peptide had a higher predicted affinity towards the blades of the propeller domain of the

protein, which has been implicated for functionality towards the ligand binding domain (Figs. S13, S14)26,27. The results indicate that these peptides are capable of retaining their native

affinity to the receptor. DISCUSSION Engineered peptides for SAPNs has become an increasingly popular modality to investigate due to its simplicity and multifunctionality. This work was

designed to examine the extent that biomolecular targeting and supramolecular morphology contribute to enhanced uptake, contrast, and toxicity of photodynamic cargo against target-positive

cells and tissue from peptide nanoparticles. We found that the SAPNs produced between our iRGD branched and iRGD spherical nanoparticles were consistent with the literature:

Electrostatically mediated peptide-small molecule assemblies preferentially adopt “checkerboard-like” patterning between complimentary ionic forces that assemble into nanofiber

networks28,29. Hydrophobic affinities between peptides and small molecules are a well-established strategy to engineer designer vesicles, micelles, and nanoparticles30. Although the native

iRGD peptide typically adopts a cyclic structure due to disulfide bridging from the cysteines at either end, the engineered peptides were not cyclized during peptide synthesis to prevent

adopting this conformation. The advantage here is that no higher secondary structures would hinder their self-assembly with methylene blue molecules, but it was necessary to investigate any

changes in their targeting capacity. In silico docking experiments revealed that these linear peptides prefer relatively similar affinity interactions to the αVβ3 protein when compared to

their cyclic relative. The modulated ROS generation and competitive inhibition studies agree with past reports that SAPNs can retain their targeting modality after assembling into higher

order formulation, but the geometries of these formulations can still lead to adverse effects with live cells. Multicolor flow cytometry results show that while much of the methylene blue is

internalized into the cells from the nanospherical formulation, more of the calcein dye remains trapped in the endosome with the fluorescence quenched, indicating that the fluorescence of

the methylene blue in the cell can be from the dye internalized in the endosome. A couple reasons may explain the nanosphere’s entrapment: (1) nanospherical shapes have a high propensity to

stay within the endosome, and (2), while hydrophobic R groups in amino acids normally aid in endosomal escape for viruses31. The hydrophobic stacking between the tryptophan residues and the

methylene blue molecules in the nanospheres most likely suppresses their exposure for any escape capabilities for the particles. The translation between live cell to live tissue efficacy,

especially with targeted delivery, can also be compromised in a variety of ways. Although previous studies have shown that co-loaded iRGD and small molecule drug formulations have led to

robust therapeutic results with simple administration, we did not expect the nanospheres to have a dampened tumor photodynamic toxicity in vivo. Nonetheless, nanoparticle formulations have

shown significant impact on the pharmacokinetics and biodistribution32. The increased fluorescence in the liver indicates preferential liver clearance of spherical nanoparticles above the

fiber nanoparticles. Liver clearance has remained as an important barrier for different kinds of nanospherical formulations, such as oncolytic viruses, lipid nanoparticles, and inorganic

nanoparticles33,34. Strategies to overcome these barriers may include surface charge tunability and switchable self-assembly for better circulation and activated targeted delivery35. We

report two tailored iRGD peptides possessing either negative residues (iRGD-DD) or hydrophobic residues (iRGD-WW). These additional amino acids in turn produce electrostatic or hydrophobic

interactions with methylene blue. Both peptides led to the self-assembly with the methylene blue producing nanoparticles. A mixture of MB and native iRGD did not. Assemblies of MB and our

two peptides concurred with similar assemblies previously reported in the literature. Electrostatically mediated assembly results in branched nanoparticles (iRGD-BNPs). The hydrophobic

affinities between peptides and MB molecules resulted in spherical nanoparticles (iRGD-SNPs). Native iRGD peptides did not produce assemblies. The product offers targeting via the iRGD

sequence, photoacoustic contrast via the methylene blue cargo, and cytotoxicity via photodynamic therapy of the methylene blue cargo. Future work will integrate additional peptide structures

to further refine the morphology of the particles for improved in vivo targeting capabilities. METHODS PEPTIDE SYNTHESIS AND PREPARATION Peptides were synthesized using standard solid-phase

synthesis (AAPTEC Eclipse) on Wang resin solid support. Peptides were cleaved with TFA:Phenol:Water:Thioanisole:EDOT (82.5:2.5:5:5:5) and washed thrice with cold diethyl ether. After

lyophilization, peptides were resuspended in water and acetonitrile (15%), and then purified via reverse phase high performance liquid chromatography (RP-HPLC) with a Shimadzu LC-40 HPLC

system equipped with a LC-40D solvent delivery module, photodiode array detector SPD-M40, and degassing unit DGU-403, using a gradient of approximately 1% of acetonitrile per minute. Pure

fractions were characterized via ESI-Mass Spectrometry, and further lyophilized before use. PEPTIDE DYE ASSEMBLIES Peptides were mixed with methylene blue in pure milliQ water at a 10:1

peptide-dye molar ratio unless specified. They were allowed to react at 250 rpm in the dark for two hours. MULTISPECTRAL NANOPARTICLE TRACKING ANALYSIS MANTA ® Multispectral Nanoparticle

Tracking Analysis (Horiba Scientific, Irvine, CA) was used for size and concentration measurements of nanoparticles and particle formation kinetics. Before each sample was measured, a blank

measurement of the reaction solvent was measured to reduce background. CELL CULTURE Human ovarian adenocarinoma (SKOV-3), epithelial human breast cancer (MCF-7), and human embryonic kidney

cells (HEK293-T) were used for the cell experiments. SKOV-3 was cultured in McCoy’s 5 A Medium, and MCF-7 and HEK 293T cells were cultured in DMEM. Medium was supplemented with 10% fetal

bovine serum (FBS) and 1% penicillin/streptomycin. Cells passaged three times before use. IN VITRO PHOTODYNAMIC CYTOTOXICITY Cells were seeded overnight in 96 well plates at 10,000 cells per

well. The 100 nM methylene blue and peptide-methylene blue assemblies were then incubated with the cells for two hours before rinsing with PBS. The amount of methylene blue was held

constant for all cases for comparison. For irradiation, a NIR laser (660 nm, 100 mW cm−2) was fixed on a ring stand and pointed to the well for 10 minutes before characterization. Dark

control samples were seeded on an entirely different plate and were kept in the dark. Cytotoxicity analysis used a resazurin assay where 10 μL of dye was added to each well after

irradiation. The cells were then incubated overnight with the resazurin before measuring color changes. IN VITRO ROS CYTOTOXICITY For ROS assays, 10 µM DCFH-DA dye was incubated with the

cells for one hour before adding 100 nM peptide-dye or dye-only with two hours of incubation. The cells were rinsed in fresh PBS twice and then irradiated to induce photothermal therapy.

Hoechst 33342 NucBlue (ThermoFisher) was added to the cells for nuclei staining. INTEGRIN VALIDATION 300,000 cells were detached with trypsin, and then washed in 1 mL ice cold buffer (PBS

with 5% BSA and 0.1% sodium azide) at 700 × _g_. The cells were then incubated with the primary αVβ3 (LM609) and αVβ5 (P1F6) mAB antibodies (20 µg/µL) (abcam) on ice for 30 min. After being

washed twice, the cells were incubated with goat anti-mouse IgG Alexa Fluor 488 for 30 min on ice in the dark. The cells were then washed twice and resuspended in 1 mL of ice-cold buffer

before flow cytometry was performed using a FITC channel. COMPETITIVE INHIBITION 300,000 cells were seeded overnight. For the competitive inhibition samples, cell cultures were first treated

with αVβ5 mABs (20 µg/µL) for 90 min. The cells were then treated with the iRGD-BNPs, iR GD-SNPs, or methylene blue. The cells were detached, collected at 700xG, and the media was decanted

while the cells were resuspended in ice cold buffer for flow cytometry using the APC channel. ENDOSOMAL ESCAPE 300,000 cells per culture were seeded overnight. The following day, the cells

were incubated with the iRGD-BNPs, iRGD-NSs, methylene blue, and the iRGD methylene blue mixture for 90 min before incubation with calcein for one hour. The cells were then rinsed, detached

with trypsin, then resuspended in ice cold buffer for flow cytometry. The FITC and APC channels were used. PHOTOACOUSTIC IMAGING PA images of in vitro samples were acquired with a Vevo

system (VisualSonics) using a 21 MHz transducer (LZ-250). Samples were loaded into 0.86 mm polyethylene tubes and fixed in parallel with a 3D printed sample holder. One tube was filled with

reaction solvent to serve as a reference. The fixed samples were placed 1 cm below the transducer in a vessel filled with water. Single wavelength scans were operated at 680 and 720 nm at a

frame rate of 20 Hz. For 3D PA images, the transducer was scanned with a stepper motor along the axial dimension of the tubes. PA spectra were taken from 680 to 900 nm with a step size of 2 

nm. ANIMAL STUDIES All mice studies described below were performed in accordance with National Institutes of Health (NIH) Guidelines approved by the Institutional Animal Care and Use

Committee (IACUC) under protocol S15050 at University of California, San Diego. Female J:NU mice of 5 weeks of age were used for all in vivo experiments. For imaging experiments, mice were

anesthetized with 1–2% isoflurane. IN VIVO PHOTOACOUSTIC IMAGING Mice were divided into six groups of three and inoculated with 800,000 SKOV3 (with 50% Matrigel/PBS v/v) intraperitoneally on

the right side at the second nipple from the lower limbs. Ten days after inoculation, the mice were intraperitoneally injected with MB, iRGD+MB, iRGD-BNPs, iRGD-Ns at 9 mg/kg. The negative

controls were not inoculated with SKOV3 cells but were injected with the iRGD-NS at the same concentration. During imaging, the animals were anesthetized with isoflurane (1–2%) and laid

supine on a heated imaging stage and the transducer was directly placed above the injection site. Subjects injected with MB, iRGD-NS, and iRGD+MB were imaged using 680 nm wavelength while

the subject injected with iRGD-BNPs was imaged using 720 nm wavelength. All mice were imaged under the same PA and ultrasound gain. IN VIVO BIOLUMINESCENCE Mice were divided into five groups

of five. Here, 800,000 SKOV3-luc cells were injected (with 50% Matrigel/PBS v/v) intraperitoneally on the right side at the second nipple from the bottom. On days 3 and 6, the mice were

intraperitoneally injected with MB, iRGD+MB, iRGD-SNPs, or iRGD-BNPs (all at 9 mg/kg) or PBS. To measure tumor burden, mice were imaged with D-luciferin on days 3, 6, and 9, with 100 mg/kg

dosage in PBS. The bioluminescence was measured and imaged via IVIS Perkin-Elmer Illumination and LivingImage software. EX VIVO After the subject was euthanized, the heart, lungs, liver,

spleen, kidneys, and tumor were harvested and rinsed in PBS. The organs were then imaged for fluorescence signal via IVIS Perkin-Elmer Illumination and LivingImage software. IN SILICO

EXPERIMENTS Molecular Operating Environment (Chemical Computing Group) was used to predict docking interactions between our peptides and αVβ3. The αVβ3 – RGD complex (PDB 1L5G) was first

added into MOE, and quick prepped to an RMS gradient of 0.01 kcal/mol/A2. Peptide structures for iRGD-DD and iRGD-WW were comprised of the molecular database made to predict and compare

their docking interactions. The database was washed and quick prepped before docking simulations. We used induced fit for method refinement, with five poses predicted for each peptide.

Docking potentials, predicted images of the complexes, and ligand interactions were analyzed. DATA AVAILABILITY Data used and analyzed during the study are available from the corresponding

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_Angew Chem. Int. Ed. Engl._ 60, 19355–19363 (2021). Article  CAS  PubMed  Google Scholar  Download references ACKNOWLEDGEMENTS R.M.B. and M.N.C. acknowledge NIH fellowship support under T32

CA153915. J.V.J. acknowledges NSF funding NSF DMR 1845683 and NIH funding via S10 OD032268. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of NanoEngineering, University of

California, San Diego, La Jolla, CA, 92093, USA Raina M. Borum, Maurice Retout, Matthew N. Creyer & Jesse V. Jokerst * Materials Science Department, University of California, San Diego,

La Jolla, CA, 92093, USA Yu-Ci Chang & Jesse V. Jokerst * Department of BioEngineering, University of California, San Diego, La Jolla, CA, 92093, USA Karlo Gregorio * Department of

Radiology, University of California, San Diego, La Jolla, CA, 92093, USA Jesse V. Jokerst Authors * Raina M. Borum View author publications You can also search for this author inPubMed 

Google Scholar * Maurice Retout View author publications You can also search for this author inPubMed Google Scholar * Matthew N. Creyer View author publications You can also search for this

author inPubMed Google Scholar * Yu-Ci Chang View author publications You can also search for this author inPubMed Google Scholar * Karlo Gregorio View author publications You can also

search for this author inPubMed Google Scholar * Jesse V. Jokerst View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS R.M.B. and M.R.

contributed equally. R.M.B. and J.V.J. conceived the research and wrote the paper. R.M.B. and M.R. designed the peptides, designed and implemented the experiments, acquired and analyzed

data, wrote the paper. M.C., Y.-C.C., and K.G. acquired data. J.V.J. supervised the research and wrote the paper. CORRESPONDING AUTHOR Correspondence to Jesse V. Jokerst. ETHICS DECLARATIONS

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nanostructures for in vivo tumor imaging and photodynamic toxicity. _npj Imaging_ 2, 4 (2024). https://doi.org/10.1038/s44303-024-00008-4 Download citation * Received: 01 November 2023 *

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