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ABSTRACT The present study aimed to determine the effect of thyroid hormone dysfunction on brown adipose tissue activity and white adipose tissue browning in mice. Twenty randomized female

C57BL/6NTac mice per treatment group housed at room temperature were rendered hypothyroid or hyperthyroid. _In-vivo_ small animal 18F-FDG PET/MRI was performed to determine the effects of

hypo- and hyperthyroidism on BAT mass and BAT activity. _Ex-vivo_14C-acetate loading assay and assessment of thermogenic gene and protein expression permitted analysis of oxidative and

thermogenic capacities of WAT and BAT of eu-, hyper and hypothyroid mice. 18F-FDG PET/MRI revealed a lack of brown adipose tissue activity in hypothyroid mice, whereas hyperthyroid mice

displayed increased BAT mass alongside enhanced 18F-FDG uptake. In white adipose tissue of both, hyper- and hypothyroid mice, we found a significant induction of thermogenic genes together

with multilocular adipocytes expressing UCP1. Taken together, these results suggest that both the hyperthyroid and hypothyroid state stimulate WAT thermogenesis most likely as a consequence

of enhanced adrenergic signaling or compensation for impaired BAT function, respectively. SIMILAR CONTENT BEING VIEWED BY OTHERS TRIIODOTHYRONINE (T3) PROMOTES BROWNING OF WHITE ADIPOSE

THROUGH INHIBITION OF THE PI3K/AKT SIGNALLING PATHWAY Article Open access 02 September 2024 BONE MARROW ADIPOSE TISSUE IS A UNIQUE ADIPOSE SUBTYPE WITH DISTINCT ROLES IN GLUCOSE HOMEOSTASIS

Article Open access 18 June 2020 TRIIODOTHYRONINE (T3) PROMOTES BROWN FAT HYPERPLASIA VIA THYROID HORMONE RECEPTOR Α MEDIATED ADIPOCYTE PROGENITOR CELL PROLIFERATION Article Open access 13

June 2022 INTRODUCTION Thyroid hormones (TH) are intimately involved in the regulation of energy metabolism. Administration of TH to mammals leads to an increase in basal metabolic rate and

thermogenesis1. The important role of TH in the regulation of body temperature homeostasis is perhaps best reflected by the cold or heat intolerance of animals and humans with hypothyroidism

or hyperthyroidism, respectively2. Under conditions of thermoneutrality, core body temperature can be maintained in the absence of any thermoregulatory process (e.g. shivering). However,

under conditions of cold exposure adaptive thermogenesis occurs through a compensatory increase in sympathetic nervous system tone (SNS) which, in concert with TH, accelerates energy

expenditure (EE) and increases body temperature3. Numerous studies primarily performed on animal models have investigated the mechanism of TH for activating brown adipose tissue (BAT).

Findings from different TR knockout mice (e.g. TRα, TRβ, TRα1+m) and the use of isoform-selective agonists indicate specific roles for TH-adrenergic interactions in BAT and white adipose

tissue(WAT)4,5,6,7,8,9,10. Early studies in thyroidectomized and hypothyroid rats demonstrated the relevance of BAT specific type II iodothyronine deiodinase Dio2 as a local and systemic

source of 3,3′,5-triiodothyronine (T3) under cold exposure and for the first time provided evidence for T3 in the enhancement of the thermogenic response to sympathetic stimulation11,12.

This suggests that the subsequent heat liberation is actually a result of synergism between norepinephrine (NE) and T3 signaling13. The significance of the central nervous system in TH

activation of BAT was demonstrated in rats in which central administration of T3, via the inhibition of hypothalamic AMP-activated protein kinase (AMPK), induces thermogenic gene expression

in BAT through stimulation of sympathetic nervous system (SNS) activity14. Additionally, SNS stimulation results in activation of β-adrenergic receptors (β-AR) by NE which induces both the

enhanced thermogenic activity of existing brown adipocytes and the recruitment of new cells to BAT depots1. Energy release is accomplished by the activity of UCP1, a BAT-specific

mitochondrial uncoupling protein1. With regards to WAT, a distinct population of UCP1-positive adipocytes arises within subcutaneous and visceral depots of mice in response to cold

exposure15,16 and β3 adrenergic receptor agonist treatment15,17,18. To date it remains a matter of controversy as to whether these cells, entitled “brite” or “beige” adipocytes, are produced

from the conversion of existing white adipocytes and/or the differentiation of WAT resident progenitor cells, leading to a process called “browning”19,20,21. There is preliminary evidence

that suggests a role for TH in the induction of beige adipocytes in WAT. In human multipotent adipose-derived stem cells, 3–10 day treatment with T3 induced UCP1 expression which was most

pronounced during the differentiation phase and was dependent on the presence of thyroid hormone receptor β (TRβ)22. Similarly, treatment of mice with the TRβ specific agonist GC-1 leads to

induction of adaptive thermogenesis in subcutaneous WAT, increased energy expenditure, and restoration of cold tolerance in cold-intolerant mice independent from BAT activity23,24.

Furthermore, in mice centrally administered T3 leads to the recruitment of BAT in inguinal WAT which is accompanied by increased energy expenditure and body temperature. Since the effect was

abolished in UCP1 KO mice, the findings highlight the significance of UCP1 as a central relais modulating the central effects of TH25. Furthermore, a novel circuit for the control of TH

feedback in the brain via the Liver X receptor α and β has recently been published26. LXRαβ−/− mice display increased TH serum level combined with changes in expression of genes associated

with TH synthesis and TH transport as well as prominent UCP1 staining in the subcutaneous adipose depot26. In a positron emission tomography (PET) study of a patient with a history of

thyroid cancer, systemic administration of TH led to BAT activation in periumbilical WAT indicating that browning had occurred as a result of T4 supplementation for 14 days27. Despite the

significance of TH for adaptive thermogenesis, there is currently limited understanding of whether and how different thyroid states, i.e. hypo- and hyperthyroidism, regulate BAT

thermogenesis as well as the thermogenic activity of WAT. Therefore, the present study aimed to determine TH induced effects on (I) BAT activity and (II) WAT browning in a mouse model of

thyroidal dysfunction with a view to incorporate the results into the context of TH-regulated effects on whole-body energy homeostasis. RESULTS BOTH HYPO- AND HYPERTHYROIDISM INDUCE BROWN

FAT BIOGENESIS Comparison of total T4 and free T3 serum concentrations between eu-, hyper- and hypothyroid mice confirmed the hyperthyroid and hypothyroid state of the animals (Fig. 1A).

This finding is further substantiated by the differential hepatic expression of the TH-responsive genes deiodinase 1 (_Dio1_), thyroxine-binding globulin (_Tbg_) and pyruvate dehydrogenase

kinase 4 (_Pdk4_) (Fig. 1B). The mRNA level of _Dio1_ and _Pdk4_ in liver increased significantly upon T4-treatment and decreased significantly in hypothyroid animals. In addition, induction

of _Tbg_, negatively regulated by TH, confirmed the hypothyroid state (Fig. 1B). Hyperthyroid mice showed slightly enhanced weight gain at the end of treatment while hypothyroid animals

displayed significantly decreased body weights (Fig. 1C). While there was no change in body composition between hyperthyroid and euthyroid animals, hypothyroid mice had significantly reduced

lean mass and concurrently increased body fat (8.9 ± 0.9%) compared with euthyroid (6.8 ± 0.3%) and hyperthyroid mice (5.7 ± 0.3%; Fig. 1D). Core body temperature was significantly

decreased in hypothyroid mice (34.9 ± 0.2 °C) compared with hyperthyroid (36.2 ± 0.2 °C) and euthyroid mice (35.7 ± 0.3 °C); Fig. 1E). Furthermore, food intake decreased in hypothyroid

animals (Fig. 1F). In view of the striking differences in core body temperature and body fat distribution between hyper- and hypothyroid mice, we next asked to which extent WAT depot

contributes to TH induced thermogenesis. Consistent with the finding of increased fat mass, adipocytes from hypothyroid mice were larger in size with increased lipid accumulation in iWAT and

gWAT when compared with hyper- and euthyroid mice (Fig. 2A). In addition we observed moderate increases in thermogenic gene program in the white fat depots of both hypo- and hyperthyroid

mice as compared to euthyroid littermates. Strikingly, _Ucp1_ gene expression was upregulated by about 40-fold in iWAT of hypo- and hyperthyroid mice compared to euthyroid controls.

Moreover, we observed a significant increase in _Ucp1_ mRNA in gWAT, which is considered to have a much lower capacity to induce thermogenic gene programs (Fig. 2B). Of interest, the zinc

finger protein _Zfp423_, which is an essential determinant of preadipocyte commitment28,29, was predominantly upregulated in WAT of hypothyroid mice (Fig. 2B). Histological examination of

the white fat depots revealed the presence of abundant islets with a distinctive multilocular appearance of adipocytes which were positively stained for UCP1 in both, hyper- and hypothyroid

states (Fig. 3A). We then investigated whether the presence of brown fat-like cells has consequences for fatty acid oxidation. As demonstrated in Fig. 3B, the highest uptake of 14C-acetate

was found in both iWAT and gWAT of hyperthyroid mice in comparison with hypo- and euthyroid mice. This was accompanied by a significantly higher level of _Ardb1_ expression in iWAT of

hyperthyroid mice, whereas the expression _Ardb1_ and _Ardb3_ in gWAT of hypo- and euthyroid mice did not change (Fig. 3C). HYPOTHYROIDISM IS ASSOCIATED WITH ENHANCED Β3-ADRENERGIC TONE IN

BAT Given the differences in core body temperature despite the activation of the thermogenic program in WAT at room temperature, we next asked whether this is due to changes in the

thyroid-adrenergic axis induced by thyroidal dysfunction. Immunohistochemical staining of tyrosine hydroxylase, which is the rate-limiting enzyme for catecholamine synthesis30, revealed no

differences in abundance in the BAT of hyperthyroid and hypothyroid mice (data not shown). Furthermore, while we did not observe changes in gene expression of the β-adrenergic receptor 1

(_Ardb1_) in BAT, there was a significant increase in gene expression of _Ardb3_ in BAT of hypo- vs hyperthyroid mice (Fig. 4A). BAT is profusely innervated by sympathetic nerve terminals

with norepinephrine (NE) acting via β-ARs31. Therefore, we next determined the concentrations of circulating NE and epinephrine in the experimental groups. Intriguingly, we found that

concentrations of NE only increased in hypothyroid mice whereas epinephrine increased in both hyperthyroid and hypothyroid mice compared to euthyroid mice (Fig. 4B). The activity of the Dio2

in hypothyroid BAT was 20-fold increased compared to hyperthyroid mice (p < 0.05). In hyperthyroid BAT Dio2 activity was significantly lower than in euthyroid controls (p < 0.01; Fig.

4C). DIFFERENT MORPHOLOGY AND GENE EXPRESSION IN BAT OF HYPO- AND HYPERTHYROID MICE In order to further unravel the discrepancies between hypothermia despite increased adrenergic signalling

and WAT browning in hypothyroid mice we next investigated BAT metabolism. Histological examination of interscapular BAT revealed gross differences in cell morphology. Whereas BAT of

euthyroid mice contained mixed regions of white and brown adipocytes, the BAT of hypothyroid mice contained predominantly adipocytes with unilocular lipid droplets of intermediate size

between WAT and BAT. Hyperthyroid BAT displayed a distinct morphology with a decreased cell size of the mainly multilocular adipocytes (Fig. 5A+B). Gene expression analysis of thermogenic

markers, including _Ucp1_, _Fgf21, Cidea, Dio2 and Elovl3,_ revealed a remarkable collective overexpression in the hypothyroid BAT compared with hyperthyroid mice (Fig. 5C). However, the

high induction of _Ucp1_ mRNA in hypothyroid mice was not reflected on the level of UCP1 protein expression. Furthermore, there was no difference in UCP1 protein expression between

hyperthyroid and euthyroid mice (Fig. 5D). In contrast, in hyperthyroid BAT we found an increased activation of β-adrenergic signaling as demonstrated by higher gene expression of the

hormone-sensitive lipase (_Hsl_) and adipose triglyceride lipase (_Atgl;_ Fig. 5C) together with an increased Ser660-phosphorylation of HSL (Fig. 5D). VOLUME AND ACTIVITY OF BAT IS

DISTINCTIVELY REGULATED IN HYPO- AND HYPERTHYROID MICE Given the magnitude of thermogenic gene expression in BAT of hypothyroid mice together with the increased stimulation of the

TH-adrenergic axis, hypothyroid mice cannot compensate for hypothermia. Therefore we next addressed whether this is due to defects in BAT activity. First, to determine the oxidative capacity

in BAT, we performed _ex-vivo_14C-acetate uptake assay and observed a significant increase in fatty acid oxidation in BAT of hyperthyroid mice (74.0 ± 14.0 dpm/count) as compared to

hypothyroid mice (18.9 ± 1.95 dpm/count; p < 0.01). There was no significant difference between hypo- and euthyroid mice (47.2 ± 14.5 dpm/count; Fig. 6A). Second, we used a combined PET-

and MR-based delineation of active BAT to investigate whether there are differences in BAT formation between the experimental mouse groups. Hyperthyroid mice contained a higher volume of

active iBAT (0.06 ccm ± 0.01 ccm) when compared to euthyroid (0.04 ccm ± 0.01 ccm; p < 0.05) and hypothyroid mice (0.03 ccm ± 0.01 ccm; Fig. 6B). We then asked whether the increase in

amount of BAT also correlates with increased BAT activity. Metabolically active BAT depots with symmetrical uptake of 18F-FDG were observed in all three groups being most marked in the

hyperthyroid group (Fig. 6C). The SUVR of 18F-FDG accumulation in iBAT was significantly decreased in hypothyroid mice vs. euthyroid mice (8.9 ± 1.3 vs. 18.9 ± 3.8; p < 0.05; Fig. 6D).

Furthermore, there was a trend towards an increased 18F-FDG accumulation in hyperthyroid compared to euthyroid and hypothyroid mice mice (21.99 ± 5.0 vs. 18.9 ± 3.8 and 8.9 ± 1.3, p = 0.06,

respectively). Finally, to determine to which extent the MR based iBAT volume is metabolically activated, the respective 18F-FDG avid iBAT with a SUV threshold of >2 was adjusted to the

MR based iBAT volume. Hyperthyroid mice presented with an active iBAT fraction of 94% the highest BAT activity. In contrast, hypothyroid mice showed only in 81% of the interscapular adipose

tissue relevant metabolic activity. In iBAT of euthyroid mice we found that 92% is activated (Fig. 6E). DISCUSSION The present study provides new evidence of how TH affects thermogenesis in

both brown and white adipose depots. For the first time with regard to metabolic activity of BAT, we were able to identify a distinct profile in hyper- and hypothyroid mice at room

temperature. First, regarding the amount of BAT, the _in vivo_18F-FDG PET/MRI-estimated BAT volumes gave first evidence for an increased total amount of BAT in hyperthyroid mice compared to

hypo- and euthyroid animals (Fig. 6B). Second, although hypo- and euthyroid animals contained detectable BAT, hyperthyroid mice displayed an increased metabolic activity as determined by

18F-FDG uptake (Fig. 6C+D). In line with this observation, fatty acid oxidation activity in BAT was increased in hyperthyroid mice as determined by the uptake of 14C-acetate (Fig. 6A). The

opposite metabolic profile was found in hypothyroid animals, which display significantly lower uptake of 18F-FDG and of 14C-acetate in BAT when compared to hyperthyroid mice (Fig. 6A–D). The

observation of active BAT in all three experimental groups emphasizes the significance of thermal conditions for metabolic control irrespective of thyroidal state. As discussed by

Nedergaard _et al_. under sub-thermal conditions (for mice 18–22 °C) BAT derived heat will be used to maintain body temperature32. Paradoxically, the low BAT activity in hypothyroid animals

was associated with a strong increase of thermogenic genes (_Ucp1_, _Dio2_, _Fgf21_, _Cidea_, and _Elovl3_; Fig. 5) and with a significant upregulation of the adrenergic system, as evidenced

by significant increases of _Ardb3_ and NE concentrations in hypothyroid animals (Fig. 4). Although NE _per se_ is not an index of NE release or sympathetic tone, these data suggest an

increase in norepinephrine outflow to the periphery as a compensatory response to maintain body temperature. This finding is principally in agreement with observations in cold exposed

hypothyroid rodents6,33. Interestingly, in iWAT and gWAT of hypothyroid mice we detected features of adipose tissue browning, evidenced by an increased expression of brown specific genes

(_Ucp1_, _Cidea_, and _Elovl3_; Fig. 2) and a multilocular UCP1-positive phenotype (Fig. 3). Collectively, these data suggest a compensatory WAT browning as a response of decreased heat

production due to BAT inactivity in hypothyroid mice. Indeed, decreased BAT thermogenesis in mice lacking bone morphogenetic protein (BMPR1A) is associated with increased circulating NE and

a compensatory browning of iWAT and eWAT in a very similar fashion to the hypothyroid mice in the present study34. The idea of heat compensation is further substantiated by the observation

of enriched expression of the _zinc finger protein 423_ (_Zfp423_) in WAT and BAT selectively in hypothyroid mice (Figs 2+5). Gupta _et al_. demonstrated that _Zfp423_+ endothelial cells

undergo an endothelial-mesenchymal transition into _Zfp423_+_Pparg_+pericytes that serve as a pool of committed pre-adipocytes29. Thus, the increased _Zfp423_ expression in the adipose

tissues of hypothyroid mice likely suggests the formation of beige adipocytes by recruitment and _de-novo_ differentiation of progenitor cells as has been demonstrated by cold exposure or

adrenergic _β__3__-AR_ agonist treatment28. The observation of browning of white adipose tissue was also made in white adipose tissues of hyperthyroid mice, where in particular, established

markers for adipose tissue browning such as _Ucp1, Fgf21_, _Cidea_, and _Elovl3_ were upregulated (Fig. 2). In addition, hyperthyroid mice were characterized by a significant increase in

_Ardb1_ expression in iWAT (Fig. 3C). Recent studies demonstrated that the absence of the _β__1__-AR_ receptor impairs NE-induced _de-novo_ brown adipogenesis in BAT35. Conversely,

_β__1__-AR_ transgenic mice are resistant to diet-induced obesity and display a high abundance of adipocytes expressing Ucp1 in WAT36. With the results gained in the present study we cannot

conclude whether or not central effects of T3 contribute to the observed WAT browning. However, evidences from a recent study by Alvarez-Crespo _et al_. suggest a coordinated action of

central hyperthyroidism on WAT browning. Thus, the authors for the first time demonstrated that central T3 administration in mice induces UCP1 dependent thermogenesis in BAT and browning of

WAT25. Also, similar to the observed increased BAT amount in our cohort of hyperthyroid mice, Alvarez-Crespo reported BAT recruitment upon central T3 infusion even at thermoneutrality25.

Notably, keeping in mind that the hyperthyroid state of our mouse cohort is rather mild it is even more interesting to observe the significant effects on BAT recruitment and WAT browning.

Further work will be necessary to address the extent of centrally induced T3 effects, i.e. by the determination of hypothalamic AMPK and ACC in our model of hyperthyroidism37. Collectively,

our data provide first evidence for differential mechanisms contributing to WAT browning dependent upon altered thyroidal state. This is most apparent in hypothyroid mice that exhibit

increased adipogenesis and _de-novo_ differentiation of brown adipocytes potentially as a compensatory mechanism to hypothermia resulting from BAT inactivity. In hyperthyroid mice, it can be

hypothesized that increased β-adrenergic activation contributes to WAT browning most likely by central effects of TH. However, it has to be emphasized that with the current data we cannot

exclude potential non cell-autonomous contributions from the SNS to adipose tissue browning irrespective of thyroidal state. Thus, in order to rule out that temperature as opposed to

thyroidal state is the underlying reason for WAT browning, further studies should be performed under temperatures where sympathetic stimulation of facultative thermogenesis is depressed i.e.

thermoneutrality (30 °C)32. METHODS MICE Nine week-old female C57BL/6NTac mice were purchased from Taconic Europe (Lille Skensved, Denmark). The mice were housed in pathogen-free facilities

in groups of three at 22 °C with a 12:12-h dark-light cycle (lights on at 06:00 h). Guidelines were approved by the local authorities of the State of Saxony, Germany as recommended by the

responsible local animal ethics review board (Regierungspräsidium Leipzig, TVV04/12, Germany). All experiments with mice were carried out according to the approved guidelines. After one week

of adaptation, induction of hyper- or hypothyroidism started at the age of ten weeks with n = 20 mice per experimental group. Mice were rendered hyperthyroid by application of L-thyroxine

at a dose of 2 μg/ml diluted in drinking water for 4 weeks38. Hypothyroidism was induced by feeding an iodine-free chow diet supplemented with 0.15% propylthiouracil (PTU, catalog TD 97061;

Harlan Teklan, Madison, WI, USA) for 4 weeks. Euthyroid mice fed standard chow diet (Altromin GmbH, Lage, Germany) served as controls. BODY COMPOSITION Body weight was recorded once a week

and after 4 weeks of treatment. Whole body composition (fat mass, lean mass and total body water) was determined in conscious mice by using nuclear magnetic resonance technology with an

EchoMRI700™ instrument (Echo Medical Systems, Houston, TX, USA). Ten animals per experimental group were measured. Rectal body temperature was measured in fed mice between 8:00–9:00 am. Mice

were sacrificed by CO2 inhalation at the age of 14 weeks. 18F-FDG PET/MRI OF BAT ACTIVATION In order to investigate the influence of thyroidal status on BAT activity, small animal

PET/magnetic resonance (MR) imaging studies were performed using a dedicated high-resolution scanner (nanoScan, Mediso Medical Imaging Systems, Hungary). Anaesthetized (induction at 4%,

maintenance at 1.8% isoflurane in 60%/40% oxygen/air) mice were injected intraperitoneally with 14.5 ± 1.3 MBq 18F-FDG followed by a PET/MR scan from 30-60 minute _post-injection_ in list

mode. The data were reconstructed dynamically into 5-min time frames (OSEM, 4 iterations, 6 subsets, MR-based attenuation and scatter correction). A T1-weighted MR image (Gradient Echo

sequence (GRE), TE = 3.2 ms (out phase), TE = 6.4 ms (in phase), TR = 15.0 ms, Flip Angle = 25°; NEX = 2) served for anatomical orientation with individual PET datasets as well as for

threshold based delineation of BAT in the intrascapular region. The 18F-FDG uptake was determined using mean standardized uptake values (SUV) in PET/MR based volumes of interest (VOIs) in

the intrascapular BAT (iBAT) and liver. To rule out an impact of alterations in the physiological 18F-FDG distribution associated with a hyperthyroid or hypothyroid state, 18F-FDG uptake was

accessed semi-quantitatively. Therefore, SUV ratios (SUVR) were calculated to normalize iBAT uptake to unspecific liver uptake39,40. Furthermore, iBAT volume was retrieved by a MR-based

delineation of fatty tissue in the intrascapular region. Active iBAT volume was defined as iBAT with an increased 18F-FDG uptake of SUV > 2. Subsequently, the fraction of active iBAT was

calculated as percentage of active iBAT volume out of total adipose tissue volume delineated by MR in the interscapular region. The SUVR and active iBAT volume between the groups were

compared using 1-way ANOVA, followed by Bonferroni’s multiple comparison test, using Prism 6.0 (GraphPad, San Diego, Ca, USA). Values of p < 0.05 were considered significant.

_EX-VIVO_14C-ACETATE LOADING ASSAY To quantify the oxidative capacity of WAT and BAT in hyper-, hypo-, and euthyroid mice, we performed an _ex-vivo_14C-acetate loading assay that measures

the incorporation of radioactively labeled acetate into triacyl glycerides (TAG) as described elsewhere41. ADIPOCYTE CELL SIZE AND NUMBER To determine cell size distribution and adipocyte

number, 200 μl aliquots of adipocyte suspension were fixed with osmic acid, incubated for 48 h at 37 °C, and counted in a Coulter counter (Multisizer III; Beckman Coulter, Krefeld,

Germany)42. SERUM CONCENTRATIONS OF THYROXINE AND SERUM METABOLITES Serum TT4 and fT3 concentrations were measured using commercial ELISA kits according to the manufacturer’s instructions

(DRG Instruments GmbH, Germany). Serum concentrations of epinephrine and norepinephrine were determined using a commercially available ELISA kit (2-CAT (A-N) Research ELISA; Rocky Mountain

Diagnostics, Inc., Colorado Springs, USA). The immunoassays were performed according to the manufacturer’s instructions. DEIODINASE TYPE 2 ACTIVITY Activity of type 2 deiodinase were

determined in duplicates in BAT homogenates (100 μg protein per 100 μl reaction) as described43,44. WESTERN BLOT ANALYSIS Western Blot analysis was performed on samples of inguinal WAT

(iWAT), gonadal WAT (gWAT) and BAT as described previously45. The following antibodies and dilutions were used: mouse polyclonal anti-Ucp1 antibody (1:500; Merck Millipore; Darmstadt,

Germany), pHSL and HSL(1:1000; Cell Signaling Technology, Danvers, USA). Blots were visualized by enhanced chemiluminescence (Pierce, Milwaukee, USA). QUANTITATIVE REAL-TIME-PCR (QPCR) For

quantification of gene expression qPCR was performed using the LightCycler System LC480 and LightCycler-DNA Master SYBR Green I Kit (Roche, Mannheim, Germany) as described previously46.

Primer sequences are available on request. Gene expression was calculated by the delta-delta CT method using _36B4_ as a reference gene47. Relative gene expression was calculated by setting

the mean of the euthyroid control group to 1 and then calculating each individual value of the three groups of mice studied. IMMUNOHISTOCHEMISTRY Tissues were harvested, fixed in 4%

paraformaldehyde (pH 7.4) for 24 h at 4 °C and subsequently embedded in paraffin. Immunohistochemical analysis was performed on BAT and WAT sections rabbit anti-UCP1 polyclonal antibody

(ab23841; Abcam, Cambridge, UK). Sections were rinsed thoroughly and incubated with labelled polymer HRP anti-rabbit (Dako Envision™+; Dako, Hamburg, Germany) for 1 h. Visualization was

performed with 3,3′-diaminobenzidine. Microscopic examination was performed using an Axio Observer Microscop (Carl Zeiss, Jena, Germany). Images were obtained using ZEN2012 software (Carl

Zeiss, Germany). STATISTICAL ANALYSES Data are shown as mean ± SEM. Statistical significance was determined by Student´s t-test for comparison of two experimental groups or by ANOVA for

comparison of multiple conditions followed by Bonferroni’s multiple comparison test, using Prism 6.0 (GraphPad, San Diego, Ca, USA). _P_ values less or equal to 0.05 were considered to be

significant. ADDITIONAL INFORMATION HOW TO CITE THIS ARTICLE: Weiner, J. _et al_. Thyroid hormone status defines brown adipose tissue activity and browning of white adipose tissues in mice.

_Sci. Rep._ 6, 38124; doi: 10.1038/srep38124 (2016). PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional

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Dr. M. Patt, Department of Nuclear Medicine, University of Leipzig, Germany, for the supply of radiotracer and Eva Böge and Viola Döbel, Department of Internal Medicine, University of

Leipzig, Germany, for technical assistance. This work was supported by the DFG SPP1629 (TO 718/2, KR425811-1, KO 922/17-1; Z KO 922/17-1/2) as well as the DFG SFB1052 (C01 and C07), from the

German Diabetes Association and from the DHFD (Diabetes Hilfs- und Forschungsfonds Deutschland). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Endocrinology and Nephrology,

University Hospital, Leipzig, Germany Juliane Weiner, Nora Klöting, Claudia Gebhardt, John T. Heiker, Susan Kralisch, Michael Stumvoll, Matthias Blüher, Anke Tönjes & Kerstin Krause *

Department of Neuroradiopharmaceuticals, Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer Research, Research Site Leipzig, Leipzig, Germany Mathias Kranz, Winnie

Deuther-Conrad & Peter Brust * University of Leipzig, IFB Adiposity Diseases, Leipzig, Germany Nora Klöting, Mohammed Hankir, Susan Kralisch, Michael Stumvoll, Matthias Blüher, Osama

Sabri & Swen Hesse * German Center for Diabetes Research (DZD), Leipzig, Germany Anne Kunath * Department of Nuclear Medicine, University Hospital, Leipzig, Germany Karen Steinhoff, 

Vilia Zeisig, Osama Sabri & Swen Hesse * Institute of Experimental Endocrinology, Charité University Hospital, Berlin, Germany Eddy Rijntjes & Josef Köhrle Authors * Juliane Weiner

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inPubMed Google Scholar * Kerstin Krause View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS J.W. and K.K. designed and performed the

experiments, interpreted and analyzed the data, and wrote the manuscript. N.K. and A.K. performed mouse work. M.K., K.S., V.Z., S.H., W.D.C., P.B., and O.S. performed and analyzed PET/MRI

analysis. E.R. and J.K. determined serum concentrations of thyroid hormones and DIO2 activity assays. C.G., S.K., J.T.H. and M.H. performed experiments and analyzed data. M.B., M.S., and

A.T. interpreted and analyzed the data, and critically edited the manuscript. All authors read, commented and approved the final version of the manuscript. K.K. is responsible for the

integrity of the work as a whole. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. RIGHTS AND PERMISSIONS This work is licensed under a Creative

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this license, visit http://creativecommons.org/licenses/by/4.0/ Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Weiner, J., Kranz, M., Klöting, N. _et al._ Thyroid hormone

status defines brown adipose tissue activity and browning of white adipose tissues in mice. _Sci Rep_ 6, 38124 (2016). https://doi.org/10.1038/srep38124 Download citation * Received: 25

February 2016 * Accepted: 07 November 2016 * Published: 12 December 2016 * DOI: https://doi.org/10.1038/srep38124 SHARE THIS ARTICLE Anyone you share the following link with will be able to

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