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ABSTRACT Pancreatic cancer is a devastating disease with poor prognosis. The association between vitamin A, retinol and carotenoid intake and the risk of pancreatic cancer occurrence remains

controversial, and therefore it is necessary to make a meta-analysis to clarify the association between vitamin A, retinol and carotenoid intake and pancreatic cancer risk. In the present

study, PubMed and EMBASE databases were used to identify qualified studies. The association between dietary vitamin A, retinol and carotenoids was estimated by pooled odds ratios (ORs) and

corresponding 95% confidence intervals (CIs). It was found that there was an inverse correlation between vitamin A, beta-carotene and lycopene intake and the risk of pancreatic cancer (for

vitamin A, pooled OR = 0.85, 95%CI = 0.74–0.97, P = 0.015; for beta-carotene, pooled OR = 0.78, 95%CI = 0.66–0.92, P = 0.003; for lycopene, pooled OR = 0.84, 95%CI = 0.73–0.97, P = 0.020),

which was more prominent in case-control study subgroup. In conclusion, dietary vitamin A, beta-carotene and lycopene might inversely correlate with pancreatic cancer. SIMILAR CONTENT BEING

VIEWED BY OTHERS DIETARY INTAKE AND TISSUE BIOMARKERS OF OMEGA-6 FATTY ACIDS AND RISK OF COLORECTAL CANCER IN ADULTS: A SYSTEMATIC REVIEW AND DOSE-RESPONSE META-ANALYSIS OF PROSPECTIVE

COHORT STUDIES Article Open access 18 April 2025 ASSOCIATION BETWEEN DIETARY VITAMIN A INTAKE FROM DIFFERENT SOURCES AND NON-ALCOHOLIC FATTY LIVER DISEASE AMONG ADULTS Article Open access 22

January 2024 THE INFLUENCE OF DIETARY VEGETABLES AND FRUITS ON ENDOMETRIAL CANCER RISK: A META-ANALYSIS OF OBSERVATIONAL STUDIES Article 23 September 2022 INTRODUCTION Pancreatic cancer is

a devastating disease with poor prognosis and the 5-year survival rate remains low at 8%1. It is the eighth and ninth leading cause of cancer-related death in men and women respectively

throughout the world2. For patients with resectable pancreatic cancer, surgery is the mainstay of treatment. But the median overall survival time remains low in all pancreatic cancer

stages3. There have been few therapeutic advances or effective treatments over the last few years4, highlighting the importance of identifying preventive factors for this malignancy. Risk

factors such as smoking, obesity, diabetes mellitus, chronic pancreatitis and established genetic syndromes are known to be associated with pancreatic cancer5. A number of epidemiologic

studies have been published in an attempt to explore the relationship between nutrient intake and the risk of pancreatic cancer occurrence. Various vitamins including vitamin B6, vitamin C7

and vitamin E8 have been implicated in the risk of pancreatic cancer occurrence according to previous studies. Vitamin A (retinol) and its derivatives are a group of fat soluble compounds

composed of a similar structure which are rich in cod liver oil and play important role in multiple biological processes9. Due to their ability to promote normal embryonic development and

exert effects on cellular differentiation, they are essential for all stages of life from embryogenesis to adulthood10. However, they cannot be synthesized de novo by animals (including

human) and must be obtained from the diets11. Recently, a myriad of epidemiological studies have demonstrated an inverse relationship between dietary vitamin A consumption and cancer

development12. For instance, vitamin A has been proved to play a protective role in breast cancer13 and lung cancer14. However, the association between vitamin A (including retinol and

carotenoid) and pancreatic cancer remains controversial15,16,17,18. Zablotska _et al_. conducted a case–control study to evaluate the association of dietary vitamin D, calcium and retinol

and the risk of pancreatic cancer in USA, finding that there was no signification association between them18. Also, Kalapothaki _et al_. found that vitamin A intake was not related to

pancreatic cancer risk when crude fiber intake was adjusted16. The results of clinical studies are not consistent with those of molecular researches. But other carotenoids, such as lycopene,

alpha-and beta-carotene, are associated with pancreatic cancer risk17,19. Therefore, a meta-analysis is necessary to clarify the association between vitamin A, retinol and carotenoids

intake and pancreatic cancer risk. RESULTS STUDY CHARACTERISTICS AND QUALITY ASSESSMENT Initially, 672 articles were identified and 18 eligible studies were included in meta-analysis (Fig.

1). The characteristics of the studies and quality assessment results are shown in Table 1. The studies were published from 1990 to 2013. Since the subjects could be divided into males and

females, the studies by Ji _et al_., Nkondjock _et al_. and Zablotska _et al_. were separated into two studies, respectively18,19,20. According to different designs of controls, the study

conducted by Kalapothaki _et al_. was also divided into two studies16. Therefore, there were altogether 22 studies in our meta-analysis, among which 16 were performed in Caucasians, 3 in

Asians and 3 in mixed population. Besides, 3 studies were conducted in males only, 3 studies were in females only, and the other 16 in both sexes As for the nutrient type, 6 studies focused

on Vitamin A, 11 on retinol, and 17 on carotenoids including 6 on alpha-carotene, 14 on beta-carotene, 8 on lycopene, 6 on crytoxanthin and 7 on lutein and zeaxanthin. Quality assessment was

conducted in all included studies, and the Newcastle-Ottawa-Scale (NOS) scores ranged from 6 to 9. QUANTITATIVE SYNTHESIS VITAMIN A AND PANCREATIC CANCER The results pooled by the fixed

effect model indicated that there was an inverse association between vitamin A intake and pancreatic cancer risk (OR = 0.85, 95%CI = 0.74–0.97, P = 0.015) (Fig. 2). In addition,

stratification analysis conducted by ethnicity and study design type revealed a significant association between vitamin A intake and pancreatic cancer risk in Caucasians subgroup (OR = 0.84,

95%CI = 0.73–0.96, P = 0.011) and case-control subgroup (OR = 0.83, 95%CI = 0.72–0.95, P = 0.007). Subsequently, publication bias was test by funnel plot and Egger’s test. The Egger’s test

value suggested that significant publication bias was in the meta-analysis (P = 0.052). The results of metatrim suggested that the summary OR was 0.815 and corresponding 95%CI was 0.702 to

0.946. Besides, no single study could change the results in sensitive analyses, implying that the results of this meta-analysis were robust. RETINOL AND PANCREATIC CANCER The meta-analysis

based on 11 studies of 9 articles indicated that there was no significant correlation between retinol intake and pancreatic cancer risk (OR = 1.02, 95%CI = 0.78–1.34, P = 0.860). Subgroup

analysis by ethnicity and the results showed no significant correlation between retinol intake and the risk of pancreatic cancer (Fig. 3). Additionally, the stability of the results was

estimated by sensitive analysis, showing that a good stability and credibility. Publication bias was also tested by funnel plot and Egger’s test (P = 0.591), suggesting that there was no

statistically significant publication bias in this meta-analysis. CAROTENE, ALPHA-CAROTENE, BETA-CAROTENE AND PANCREATIC CANCER Overall, there was an inverse correlation between carotene

intake and pancreatic cancer risk (OR = 0.77, 95%CI = 0.67–0.89, P < 0.001). After stratification by ethnicity, the association became stronger among Asian population (OR = 0.55, 95%CI = 

0.37–0.80, P = 0.002) as compared with that in Caucasian (OR = 0.82, 95%CI = 0.70–0.96, P = 0.016) and the mixed population (OR = 0.72, 95%CI = 0.56–0.94, P = 0.016) (Fig. 4). The result of

case-control subgroup showed an inverse association between carotene intake and pancreatic cancer risk (OR = 0.74, 95%CI = 0.62–0.87, P < 0.001), while there was no significant

association between carotene intake and pancreatic cancer risk in prospective studies (OR = 0.94, 95%CI = 0.76–1.16, P = 0.577). As for nutrient types, the result showed that there was an

inverse correlation between beta-carotene intake and pancreatic cancer risk (OR = 0.78, 95%CI = 0.66–0.92, P = 0.003) (Fig. 5), but no significant correlation was observed between

alpha-carotene intake and pancreatic cancer risk (OR = 0.88, 95%CI = 0.66–1.18, P = 0.405) (Fig. 6). Sensitive analysis indicated that the results of this meta-analysis were stable.

Publication bias was estimated by forest plot and Egger’s test (P = 0.170) and no significant publication bias was found in carotene meta-analysis (Fig. 7). LYCOPENE AND PANCREATIC CANCER As

shown in Fig. 8, there was an inverse correlation between lycopene intake and pancreatic cancer risk (OR = 0.84, 95%CI = 0.73–0.97, P = 0.020). When stratified by ethnicity, there was an

inverse relationship between lycopene intake and pancreatic cancer risk in Caucasians (OR = 0.86, 95%CI = 0.73–1.00, P = 0.05), while this correlation was insignificant in the mixed

population (OR = 0.78, 95%CI = 0.54–1.13, P = 0.187). With respect to the study design type, decreased the pancreatic cancer risk in case-control study (OR = 0.77, 95%CI = 0.64–0.92, P = 

0.005), while prospective study showed no association between lycopene intake and pancreatic cancer (OR = 0.98, 95%CI = 0.78–1.23, P = 0.844). The sensitive analysis revealed that the

results of this meta-analysis were credible and stable. Publication bias was evaluated by forest plot and Egger’s test (P = 0.857), and the results showed no significant publication bias in

the meta-analysis. CRYPTOXANTHIN AND PANCREATIC CANCER The results showed no significant association between cryptoxanthin intake and pancreatic cancer risk (OR = 0.86, 95%CI = 0.67–1.12, P 

= 0.276). After stratification by ethnicity, there was no significant association in Caucasians and the mixed population. Similarly, there was no significant correlation in Caucasians and

the mixed population. Subgroup analysis showed no significant correlation between cryptoxanthin intake and pancreatic cancer risk in case-control study and prospective study. The sensitive

analysis implied that the result of the meta-analysis was robustness. The forest plot and Egger’s test (P = 0.522) suggested that no significant publication bias in this meta-analysis.

LUTEIN AND ZEAXANTHIN AND PANCREATIC CANCER Pooled ORs and corresponding 95%CIs indicated that there was no significant correlation between lutein and zeaxanthin intake and pancreatic cancer

risk (OR = 0.80, 95%CI = 0.61–1.05, P = 0.104). As it showed in Fig. 9, there was an inverse association between lutein and zeaxanthin intake and pancreatic cancer risk in the mixed

population (OR = 0.61, 95%CI = 0.42–0.89, P = 0.010), but not in Caucasians population (OR = 0.84, 95%CI = 0.62–1.3, P = 0.251). Besides, subgroup analysis showed no significant association

could be found in the subgroup of case-control study and prospective study. The sensitive analysis implied that this result was robust. Furthermore, the forest plot and Egger’s test (P = 

0.664) showed no significant publication bias in our meta-analysis. In summary, there was an inverse correlation between vitamin A (including some carotenoids) intake and pancreatic cancer

risk, but no significant correlation was observed between retinol intake and pancreatic cancer risk. All the results were summarized in Table 2. DISCUSSION This meta-analysis included 18

articles focusing on the correlation between vitamin A, retinol and carotenoid intake and pancreatic cancer risk. The result showed that dietary vitamin A, carotene, beta-carotene and

lycopene were inversely correlated with the risk of pancreatic cancer risk. However, retinol, alpha-carotene, cryptoxanthin, lutein and zeaxanthin intake had no relationship with pancreatic

cancer risk. Vitamin A is a necessity for cell growth and differentiation of epithelial tissues and must be obtained from diets in the human body. Provitamin A compounds, such as

beta-carotene can transform into vitamin A, which is an essential molecule entailing multiple developmental pathways and influencing cell proliferation and differentiation in a variety of

cell types21. Molecular studies had demonstrated that retinoids (vitamin A and its metabolites) could cause apoptosis in pancreatic cancer cells and thus suppress pancreatic cancer growth

via activation of retinoic acid receptor-gamma, suggesting that vitamin A and its metabolites may play a protective role against pancreatic cancer22. Additionally, several preclinical

studies showed that retinols play roles in many signaling pathways related with cell growth, adhesion and migration23,24,25. A recent study revealed that retinoic acid could inhibit

pancreatic cancer cell migration and epithelial-mesenchymal transition by decreasing the expression of interleukin 6 (IL-6) in cancer-associated fibroblast (CAFs) cells25, suggesting that

retinoids could be applied for prevention or therapy the recurrence and metastasis of pancreatic cancer. Actually, immunotherapy including 13-cis-retinoic acid and interleukin 2 had been

used for treating locally advanced pancreatic cancer26. However, there is no clinical study focusing on vitamin A therapy in pancreatic cancer so far. Other carotenoids such as lycopene and

zeaxanthin that cannot convert into vitamin A may act as antioxidants against cancer initiation and progression. Lycopene has been proved to be a potent inhibitor for cell proliferation and

growth in some cancer cells, such as endometrial cancer, breast cancer and lung cancer27,28. More recently, Assar and colleagues reported that lycopene could suppress the nuclear factor

kappa B (NF-κB) signaling pathway through inhibiting phosphorylation of inhibitor of kappa B (IκB) in human prostate and breast cancer cells, probably due to the action of lycopene as an

antioxidant to scavenge free radicals29. These data provide a potential strategy to prevent and treat pancreatic cancer by using lycopene. Several meta-analyses or pooled analyses have

investigated the association between the intake of other vitamins and pancreatic cancer risk. Fan _et al_. conducted a meta-analysis to assess the relationship between dietary vitamin C and

pancreatic cancer risk and found that a higher vitamin C intake was inversely correlated with pancreatic cancer risk7. Similarly, dietary vitamin E was found to be a protective factor

against pancreatic cancer8. However, a recent pooled analysis suggested that higher levels of vitamin D might increase pancreatic cancer risk30. To the best of our knowledge, this is the

first meta-analysis about the relationship of dietary vitamin A, retinol, and carotenoids with pancreatic cancer risk. Overall, we found that dietary vitamin A had an inversely association

with pancreatic cancer risk. On the contrary, several studies had investigated the relationship between vitamin A intake and the risk of pancreatic cancer and the results were negative.

Partly, these conclusions may due to the small sample size of each study. Besides, the only prospective study showed no association between vitamin A intake and the risk of pancreatic

cancer31. However, this prospective study was based on male smokers instead of the general population. No significant relationship was found between dietary retinol and pancreatic cancer

risk, or in the subgroups of Caucasians, Asians and the mixed population. These results are consistent with previous case-control studies15,17,32,33,34,35. The association between carotenoid

intake and cancer risk had been investigated in many cancer types. Zhou _et al_. conducted a meta-analysis and found that beta-carotene and alpha-carotene were inversely correlated with

risk of gastric cancer36. Another meta-analysis revealed that dietary alpha-carotene and lycopene could decrease the risk of prostate cancer37. In our meta-analysis, beta-carotene and

lycopene intake were inversely associated with pancreatic cancer risk, while alpha-carotene and cryptoxanthin intake had no significant relationship with pancreatic cancer risk. Many

previous observational studies including 10 case-control studies15,17,19,32,33,38,39,40,41 and 4 prospective studies31,42,43,44 on the relationship between beta-carotene intake and

pancreatic cancer risk reported inconsistent results. These discrepant results from case-control studies might result from recall bias of self-reported dietary intake and different

ethnicity. However, the results of 4 prospective studies did not suggest that beta-carotene acted as a protective factor against pancreatic cancer. Notably, a nested case–control study

performed in Europe indicated that higher plasma concentrations of beta-carotene could decrease the risk of suffering from pancreatic cancer45. Besides, this article also suggested that

higher plasma concentrations of zeaxanthin might be inversely related to pancreatic cancer risk, which is consistent with the result of our meta-analysis indicated that the relationship

between the total lutein and zeaxanthin intake and pancreatic cancer risk in the subgroup of the mixed population. However, this subgroup result was based on only one case-control study38.

Between-study heterogeneity is common in meta-analysis46, and the heterogeneity test showed moderate between-study heterogeneity in most meta-analyses. The study characteristics of each

study may lead to heterogeneity. In our meta-analysis, the study design, geographic location, publication year and sources of control and cases are various. To find the causes of

heterogeneity for covariates, we conducted a meta-regression and subgroup analysis. As a result, meta-regression failed to determine any study characteristics including publication year,

study type, study size and ethnicity as sources of heterogeneity. Subgroup analyses by study type and ethnicity were performed to explore the source of heterogeneity. However, between-study

heterogeneity was permanent in some subgroups, suggesting that other unknown confounding factors may be present. In addition, the adjustments and the intake levels of nutrients are different

between these studies. Although the results obtained in our meta-analysis are statistically significant as a whole, several limitations should be noted in interpreting our study. First,

most included studies in our meta-analysis were case-control studies, in which recall bias may be unavoidable. Both case-control and cohort study are observational study, they require fewer

resources but provide less evidence compared with randomized controlled trial (RCT). However, given the extremely low morbidity of pancreatic cancer, there is too difficult to conduct RCT on

the association between vitamin A, retinol and carotenoid intake and the risk of pancreatic cancer. Second, some confounding factors such as eating habits and residual confounding cannot be

measured, which may affect the stability and credibility of our meta-analysis. Third, the individual sample sizes for each case in most studies included in this meta-analysis were

relatively small and these studies were conducted in different populations whose heredity might be different. Finally, some pancreatic cancer cases may be familial heredity47, which may

change the morbidity of pancreatic cancer and lead to an inaccurate results in epidemiological study. In conclusion, the results of our meta-analysis indicate that high-level vitamin A,

carotene, beta-carotene and lycopene intake might be the potential factors related to low pancreatic cancer risk. However, due to the limitations of the present meta-analysis mentioned

above, it should be prudent to make recommendations based on the results of the present meta-analysis. MATERIALS AND METHODS LITERATURE SEARCH STRATEGY PubMed and EMBASE databases were used

to identify observational studies that reported the association between vitamin A, retinol and carotenoid intake and the risk of PANCREATIC CANCER up to December 30th, 2015 by using the

following key words “Vitamin A or Vitamin or diet or dietary or retinol or carotenoids or carotene or cryptoxanthin or lycopene or lutein or zeaxanthin” and “pancreatic” and “cancer or

carcinoma or neoplasm or tumor or adenocarcinoma”. Additionally, some potential studies were identified via secondary searches which were conducted by searching reference lists of selected

literatures. INCLUSION AND EXCLUSION CRITERIA The inclusion criteria were: (1) observational studies including case-control and cohort study design; (2) studies reporting the association

between exposure factors including vitamin A, retinol and carotenoids and the risk of pancreatic cancer; (3) studies published in English or Chinese; (4) Providing the odds ratio (OR) (or

relative risk [RR], hazard risk [HR]) data and the corresponding 95% corresponding interval (CI) for the highest vs. the lowest level of vitamin A intake or retinol intake or other

carotenoid intake. The exclusion criteria were: (1) reviews, meta-analyses, case reports, editorials or human-uncorrelated experiments; (2) duplicated study (If duplicated studies were

present, the study with the largest sample size was selected); (3) studies not reporting OR(or RR, HR) and 95%CI or lacking sufficient data to calculate OR(or RR and HR) and 95%CI. DATA

EXTRACTION The process of data extraction was conducted by two authors independently with a standardized form based on the inclusion and exclusion criteria mentioned above. Any divergence

was resolved by rechecking until consensus was reached. The following information was collected: the last name of the first author, publication year, country, ethnicity, study design, number

of cases and controls or total sample size, carotenoid types, OR(or RR, HR), the corresponding 95% CI from the most fully adjusted model for the highest vs. the lowest vitamin A intake, and

the factors of adjustment for covariates. QUALITY ASSESSMENT Newcastle-Ottawa-Scale (NOS) was applied in quality assessment48. During this process, the quality of the selected observational

studies was evaluated independently by two authors. The NOS is a nine-point scale containing three parts: selection (four points), comparability (two points) and exposure/outcome assessment

(three points). A study with a NOS score ≥6 was regarded as a high-quality study, and vice versa. STATISTICAL ANALYSIS If the outcome under study is rare in all populations and subgroups

under review, one can generally ignore the distinctions between the various measures of relative risk49. Given the low absolute risk of pancreatic cancer in the general populations, we

interpreted all risk estimates as OR for simplicity. The relationship between vitamin A, retinol and other carotenoids and the risk of pancreatic cancer was assessed by calculating pooled OR

and 95%CI respectively. Additionally, subgroup analysis was conducted by study design type and ethnicity if sufficient data was provided. All the statistical tests were two-sided and the

results were considered as statistically significant if P ≤ 0.05. The heterogeneity test was performed by Q test and _I__2_. When _I__2_ > 50%, the random effect model was suggested to

calculate the pooled OR and 95%CI. Otherwise, the fixed effect model was applied. Furthermore, sensitivity analysis was conducted by omitting each study once a time. We also assessed

publication bias via funnel plots and Egger’s test50. This meta-analysis was performed by STATA12.0 (STATA Corporation, College Station, TX). ADDITIONAL INFORMATION HOW TO CITE THIS ARTICLE:

Huang, X. _et al_. Association between vitamin A, retinol and carotenoid intake and pancreatic cancer risk: Evidence from epidemiologic studies. _Sci. Rep._ 6, 38936; doi: 10.1038/srep38936

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ACKNOWLEDGEMENTS This work was supported by the National Natural Scientific Foundation of China (No. 81172077) and Shanghai Municipal Commission of Health and Family Planning, Key Developing

Disciplines (No. 2015ZB0202). AUTHOR INFORMATION Author notes * Huang Xiaoyi, Gao Yisha and Zhi Xiaosong contributed equally to this work. AUTHORS AND AFFILIATIONS * Department of

Pathology, Changhai Hospital, Second Military Medical University, Shanghai, P.R. China Xiaoyi Huang, Yisha Gao, Na Ta, Hui Jiang & Jianming Zheng * Department of Cell Biology, Second

Military Medical University, Shanghai, P.R. China Xiaosong Zhi Authors * Xiaoyi Huang View author publications You can also search for this author inPubMed Google Scholar * Yisha Gao View

author publications You can also search for this author inPubMed Google Scholar * Xiaosong Zhi View author publications You can also search for this author inPubMed Google Scholar * Na Ta

View author publications You can also search for this author inPubMed Google Scholar * Hui Jiang View author publications You can also search for this author inPubMed Google Scholar *

Jianming Zheng View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS The study designed by J.M.Z. Searched databases and collected full-text

papers by X.Y.H. and Y.S.G. Extracted and analyzed the data by X.Y.H., Y.S.G. and X.S.Z. Statistical analyses by X.S.Z., H.J. and T.N. The main manuscript text wrote by X.Y.H., X.S.Z. and

H.J. All authors reviewed the manuscript. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. RIGHTS AND PERMISSIONS This work is licensed under a

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view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Huang, X., Gao, Y., Zhi, X. _et al._ Association

between vitamin A, retinol and carotenoid intake and pancreatic cancer risk: Evidence from epidemiologic studies. _Sci Rep_ 6, 38936 (2016). https://doi.org/10.1038/srep38936 Download

citation * Received: 24 February 2016 * Accepted: 16 November 2016 * Published: 12 December 2016 * DOI: https://doi.org/10.1038/srep38936 SHARE THIS ARTICLE Anyone you share the following

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