Reader TTS

ABSTRACT OBJECTIVE: We investigated the effects of low-dose leptin treatment on restoration of fertility in young adult male leptin deficient obese mice. EXPERIMENTAL DESIGN AND RESULTS:

MMTV-TGF-α _Lep__ob__ Lep__ob_ mice (8–10 weeks old) were treated with recombinant mouse leptin. In experiment 1, four mice (5 µg/g body weight leptin followed by 2.5 µg/g) lost weight and

impregnated females (number of pregnancies/number of females, 3/6, 5/6, 5/10, 4/10). In experiment 2, Leptin-Obese (2.5 µg/g) and Control-Lean mice weighed significantly less than

Control-Obese mice. Epididymal pad weights of Control-Obese mice were the heaviest, followed by those of Leptin-Obese mice, and Control-Lean mice were the lightest. Testes weight was greater

in Control-Lean _vs_ Control-Obese mice. Leptin-Obese mice had testes weight not significantly different from either control group. Four of five Leptin-Obese mice impregnated females (4/10,

5/10, 2/10, 5/12, 0/10). CONCLUSIONS: These results indicate that low-dosage mouse recombinant leptin treatment restored fertility to young _Lep__ob__ Lep__ob_ male mice. Although body

weights of Leptin-Obese mice were similar to those of lean age-matched mice, epididymal fat pad weights were heavier. Access through your institution Buy or subscribe This is a preview of

subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $259.00 per year only

$21.58 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout

ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS SWITCHING FROM HIGH-FAT DIET

TO NORMAL DIET AMELIORATE BTB INTEGRITY AND IMPROVE FERTILITY POTENTIAL IN OBESE MALE MICE Article Open access 29 August 2023 HIGH FAT DIET-INDUCED OBESITY PROLONGS CRITICAL STAGES OF THE

SPERMATOGENIC CYCLE IN A LDLR−/−.LEIDEN MOUSE MODEL Article Open access 11 January 2022 MATERNAL AND PATERNAL OBESITY DIFFERENTIALLY REPROGRAM THE OVARIAN MITOCHONDRIAL BIOGENESIS OF F1

FEMALE RATS Article Open access 19 September 2023 REFERENCES * Ingalls AM, Dickie MM, Snell GD . Obese, a new mutation in the house mouse _J Hered_ 1950 41: 317–318. Article  CAS  Google

Scholar  * Jones N, Harrison GA . Genetically determined obesity and sterility in the mouse. In: Harrison RG (ed) _Studies on Fertility._ Blackwell Scientific: Oxford 1957 pp 51–64. Google

Scholar  * Pelleymounter MA, Cullen MJ, Baker MB, Hecht R, Winters D, Boone T, Collins F . Effects of the _obese_ gene product on body weight regulation in _ob/ob_ mice _Science_ 1995 269:

540–543. Article  CAS  Google Scholar  * Halaas JL, Gajiwala KS, Maffei M, Cohen SL, Chait BT, Rabinowitz D, Lallore RL, Burley SK, Freidman JM . Weight-reducing effects of the plasma

protein encoded by the _obese_ gene _Science_ 1995 269: 543–546. Article  CAS  Google Scholar  * Campfield LA, Smith FJ, Guisez Y Devos R, Burn P . Recombinant mouse OB protein: evidence for

a peripheral signal linking adiposity and central neural network _Science_ 1995 269: 546–549. Article  CAS  Google Scholar  * Weigle DS, Bukowski TR, Foster DC, Holderman S, Kramer JM,

Lasser G, Lofton-Day CE, Prunkard DE, Raymond C, Kuijper JL . Recombintant _ob_ protein reduces feeding and body weight in the _ob/ob_ mouse _J Clin Invest_ 1995 96: 2065–2070. Article  CAS

  Google Scholar  * Harris RBS, Zhou J, Redmann SM Jr, Smagin GN, Smith SR, Rodgers E, Zachwieja JJ . A leptin dose–response study in obese (_ob_/_ob_) and lean (+/?) mice _Endocrinology_

1998 139: 8–19. Article  CAS  Google Scholar  * Sarmiento U, Benson B, Kaufman S, Ross L, Qi M, Scully S, DiPaima C . Morphologic and molecular changes induced by recombinant human leptin in

the white and brown adipose tissues of C57BL/6 mice _Lab Invest_ 1997 77: 243–256. CAS  PubMed  Google Scholar  * Huang Q, Viale A, Picard F, Nahon JL, Richard D . Effects of leptin on

melanin-concentrating hormone expression in the brain of lean and obese Lepob/Lepob mice _Neuroendocrinology_ 1999 69: 145–153. Article  CAS  Google Scholar  * Chehab FF, Lim ME, Lu R .

Correction of the sterility defect in homozygous obese female mice with the human recombinant leptin _Nature Genet_ 1996 12: 318–320. Article  CAS  Google Scholar  * Mounzih K, Lu R, Chehab

FF . Leptin treatment rescues the sterility of genetically obese _ob_/_ob_ males _Endocrinology_ 1997 138: 1190–1193. Article  CAS  Google Scholar  * Gloaguen I, Costa P, Demartis A, Lazzaro

D, Di Marco A, Graziani R, Paonessa G, Chen F, Rosenblum CI, Van de Pleog LHT, Cortese R, Ciliberto G, Laufer R . Ciliary neurotrophic factor corrects obesity and diabetes associated with

leptin deficiency and resistance _Proc Natl Acad Sci USA_ 1997 94: 6456–6461. Article  CAS  Google Scholar  * Giese K, Fantl WJ, Vitt C, Stephans JC, Cousens L, Wachowicz M, Williams LT .

Reduction of food intake and weight gain by the ob protein requires a specific secondary structure and is reversible _Mol Med_ 1996 2: 50–58. Article  CAS  Google Scholar  * Matsui Y, Halter

SA, Holt JT, Hogan BLM, Coffey RJ . Development of mammary hyperplasia and neoplasia in MMTV-TGFα transgenic mice _Cell_ 1990 61: 1147–1155. Article  CAS  Google Scholar  * Picard F,

Richard D, Huang Q, Deshaies Y . Effects of leptin on adipose tissue lipoprotein lipase in the obese _ob_/_ob_ mouse _Int J Obes Relat Metab Disord_ 1998 22: 1088–1095. Article  CAS  Google

Scholar  * Hoggard N, Hunter L, Trayhurn P, Williams LM, Mercer JG . Leptin and reproduction _Proc Nutr Soc_ 1998 57: 421–427. Article  CAS  Google Scholar  * Banks WA, McLay RN, Kastin AJ,

Sarmiento U, Scully S . Passage of leptin across the blood–testis barrier _Am J Physiol_ 1999 276: E1099–E1104. CAS  PubMed  Google Scholar  * Tena-Sempere M, Pinilla L, Gonzalez LC, Dieguez

C, Casanueva FF, Aguilar E . Leptin inhibits testosterone secretion from adult rat testis _in vitro_ _J Endocrinol_ 1999 161: 211–218. Article  CAS  Google Scholar  * Licinio J, Mantzoros

C, Negrao AB, Cizza G, Wong ML, Bongiorno PB _et al_. Human leptin levels are pulsatile and inversely related to pituitary-adrenal function _Nature Med_ 1997 3: 575–579. Article  CAS  Google

Scholar  Download references ACKNOWLEDGEMENTS This study was supported by grant award DAMD17-97-1-7055 and funds from the Hormel Foundation. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *

The Hormel Institute, University of Minnesota, Austin, Minnesota, USA MP Cleary, HM Bergstrom, TL Dodge, SC Getzin, MK Jacobson & FC Phillips Authors * MP Cleary View author publications

You can also search for this author inPubMed Google Scholar * HM Bergstrom View author publications You can also search for this author inPubMed Google Scholar * TL Dodge View author

publications You can also search for this author inPubMed Google Scholar * SC Getzin View author publications You can also search for this author inPubMed Google Scholar * MK Jacobson View

author publications You can also search for this author inPubMed Google Scholar * FC Phillips View author publications You can also search for this author inPubMed Google Scholar

CORRESPONDING AUTHOR Correspondence to MP Cleary. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Cleary, M., Bergstrom, H., Dodge, T. _et al._

Restoration of fertility in young obese (_Lep__ob__ Lep__ob_) male mice with low dose recombinant mouse leptin treatment. _Int J Obes_ 25, 95–97 (2001).

https://doi.org/10.1038/sj.ijo.0801525 Download citation * Received: 04 February 2000 * Revised: 04 August 2000 * Accepted: 20 September 2000 * Published: 25 January 2001 * Issue Date: 01

January 2001 * DOI: https://doi.org/10.1038/sj.ijo.0801525 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a

shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * leptin * obese * male mice *

fertility * _Lep_ _ob_ _ Lep_ _ob_