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ABSTRACT LIGHT is a primary environmental signal regulating plant growth and form1. The receptor phytochrome responds to red light and induces changes in membrane properties and gene

expression2,3 through an unknown transduction pathway which is the subject of intense study. Etiolated wheat leaf protoplasts swell in response to red light4, by a mechanism believed to be

similar to that involved in phytochrome-regulated leaf growth and unrolling5–7. Here we report that this physiological response is preceded by a transient increase in free calcium in the

cytosol, followed by a decrease to below resting level. The timing of this response varied between protoplasts. Cytosolic calcium transients induced by photolytic release of calcium or

inositol 1,4,5-trisphosphate from chemically caged forms8–13 inside these protoplasts resulted in red light-induced increases in protoplast volume being mimicked. Our results support the

hypothesis that phytochrome-mediated signals are transduced through calcium and help to resolve a long-standing issue in plant physiology. Access through your institution Buy or subscribe

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LIGHT REGULATES STOMATAL DEVELOPMENT BY MODULATING PARACRINE SIGNALING FROM INNER TISSUES Article Open access 07 June 2021 CALCIUM SPIKES, WAVES AND OSCILLATIONS IN PLANT DEVELOPMENT AND

BIOTIC INTERACTIONS Article 29 June 2020 GROWTH IN FLUCTUATING LIGHT BUFFERS PLANTS AGAINST PHOTORESPIRATORY PERTURBATIONS Article Open access 03 November 2023 REFERENCES * Hart, J. W.

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Molecular Biology, University of Edinburgh, Rutherford Building, Edinburgh, EH9 3JH, UK P. S. Shacklock, N. D. Read & A. J. Trewavas Authors * P. S. Shacklock View author publications

You can also search for this author inPubMed Google Scholar * N. D. Read View author publications You can also search for this author inPubMed Google Scholar * A. J. Trewavas View author

publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Shacklock, P., Read, N. &

Trewavas, A. Cytosolic free calcium mediates red light-induced photomorphogenesis. _Nature_ 358, 753–755 (1992). https://doi.org/10.1038/358753a0 Download citation * Received: 05 June 1992 *

Accepted: 20 July 1992 * Issue Date: 27 August 1992 * DOI: https://doi.org/10.1038/358753a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content:

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