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ABSTRACT Catechol-_O_-methyltransferase (COMT) is an enzyme that inactivates catecholamines. Several studies have suggested that this enzyme may play a role in blood pressure regulation. We

previously reported that the expression levels of _Comt_ mRNA in Dahl salt-sensitive (DS) rats were lower than those in Lewis (LEW) rats. However, the physiological significance of this

phenomenon has not been investigated. The purpose of the present study was to evaluate the significance of lower expression of _Comt_ in Dahl salt-sensitive hypertension. The _Comt_ gene in

DS rats has a palindromic insertion in 3′-untranslated region, which appears to be responsible for reduced mRNA stability. A genome-wide quantitative trait loci (QTL) analysis of blood

pressure using 107 F2 rats indicated that a statistically significant QTL for pulse pressure was located at the _Comt_ locus in chromosome 11. Microarray analysis confirmed that _Comt_ was

the only gene differentially expressed between DS and LEW rats in this chromosomal region. However, COMT inhibitors had no significant effects on blood pressure in either DS or LEW rats.

Thus, _Comt_ was excluded from the candidate genes contributing to salt-sensitive hypertension in DS rats. A true gene responsible for pulse pressure in this chromosome 11 region remains to

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expressed loci. _Nature_ 2005; 435: 637–645. Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Kazuaki Kajimoto, Yumiko Hiura and Toshiki Sumiya: These

authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Department of Epidemiology, Research Institute, National Cardiovascular Center, Suita, Japan Kazuaki Kajimoto, Yumiko

Hiura, Toshiki Sumiya, Naomi Yasui, Tomohiko Okuda & Naoharu Iwai Authors * Kazuaki Kajimoto View author publications You can also search for this author inPubMed Google Scholar * Yumiko

Hiura View author publications You can also search for this author inPubMed Google Scholar * Toshiki Sumiya View author publications You can also search for this author inPubMed Google

Scholar * Naomi Yasui View author publications You can also search for this author inPubMed Google Scholar * Tomohiko Okuda View author publications You can also search for this author

inPubMed Google Scholar * Naoharu Iwai View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Naoharu Iwai. RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kajimoto, K., Hiura, Y., Sumiya, T. _et al._ Exclusion of the Catechol-_O_-Methyltransferase Gene from Genes

Contributing to Salt-Sensitive Hypertension in Dahl Salt-Sensitive Rats. _Hypertens Res_ 30, 459–467 (2007). https://doi.org/10.1291/hypres.30.459 Download citation * Received: 20 September

2006 * Accepted: 20 December 2006 * Issue Date: 01 May 2007 * DOI: https://doi.org/10.1291/hypres.30.459 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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KEYWORDS * _Comt_ * quantitative trait loci * genetics * hypertension * salt