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You have full access to this article via your institution. Download PDF Collier, A.J. _et al_. _Cell Stem Cell_ 20, 874–890 (2017). Human pluripotent stem cells (hPSCs) can exhibit different

degrees of pluripotency. hPSCs induced from adult somatic cells tend to exist in the so-called primed state, which resembles an advanced epiblast (tissue that will form the embryo proper),

while a naïve state resembling the early epiblast can be established by culturing cells directly from young embryos or by converting primed cells. In an effort to better identify naïve hPSCs

and clarify the results of conversion protocols, Collier _et al_. screened a panel of 486 antibodies as cell-surface markers of pluripotency. They identified a large set of antibodies that

are specific to primed cells, a set that was common to both states, and a group of eight antibodies that are largely specific to naïve hPSCs. The naïve-specific antibodies will help with

cell identification and screening for improved culture conditions. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Separating the primed from the naïve.

_Nat Methods_ 14, 768 (2017). https://doi.org/10.1038/nmeth.4376 Download citation * Published: 01 August 2017 * Issue Date: 01 August 2017 * DOI: https://doi.org/10.1038/nmeth.4376 SHARE

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