Reader TTS

Access through your institution Buy or subscribe Nitric oxide, produced by iNOS, is required for IgA class-switch recombination in the small-intestinal lamina propria of mice. While

investigating the role of iNOS in the gut, the authors found a population of iNOS+ cells in intestinal lamina propria preparations from wild-type mice that was absent in mice lacking B

cells. Further examination of these iNOS+ cells showed that they expressed IgA and had undergone class-switch recombination, suggesting that these particular iNOS+ cells are a unique subset

of IgA+ plasma cells. Interestingly, these cells also produced TNF and expressed LY6C and LY6G, features usually associated with monocytic cells. Not all IgA+ cells expressed iNOS and/or

TNF, but those that did had a different morphology and cellular IgA localization compared with IgA+iNOS−TNF− cells, suggesting that some B cells in the gut adopt a more monocyte-like

phenotype as they differentiate into IgA+ plasma cells. But how are these cells induced? The authors found that the microbiota was necessary for the expression of iNOS by IgA+ cells, and

reconstitution of germ-free mice with a single bacterial species was sufficient for the induction of IgA+iNOS+ cells. Furthermore, only gut stroma from specific pathogen-free mice, and not

bone marrow-derived stroma or gut stroma from germ-free mice, could support the development of IgA+iNOS+ cells _in vitro_. This is a preview of subscription content, access via your

institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this

article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in

* Learn about institutional subscriptions * Read our FAQs * Contact customer support ORIGINAL RESEARCH PAPER * Fritz, J. H. et al. Acquisition of a multifunctional IgA+ plasma cell phenotype

in the gut. _Nature_ 11 Dec 2011 (doi:10.1038/nature10698) Article  Google Scholar  Download references Authors * Olive Leavy View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Leavy, O. Multifunctional gut IgA+ plasma cells. _Nat Rev Immunol_ 12, 75 (2012).

https://doi.org/10.1038/nri3157 Download citation * Published: 13 January 2012 * Issue Date: February 2012 * DOI: https://doi.org/10.1038/nri3157 SHARE THIS ARTICLE Anyone you share the

following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer

Nature SharedIt content-sharing initiative