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ABSTRACT Peyronie's disease is a fibrotic disorder, a condition characterized by cellular proliferation and excess extracellular matrix production. Previous work in related conditions

has demonstrated chromosomal instability. This investigation was undertaken to analyze fibroblasts derived from Peyronie's disease tunical tissue for abnormalities of chromosome number

and progression of cytogenetic aberrations during cell culture. Tunical tissue was excised from men with Peyronie's disease from both plaque and nonplaque tissue and cells were

explanted in culture. Control cells were derived from both neonatal foreskins and normal tunica from men with congenital penile curvature. Fluorescent _in situ_ hybridization was used to

probe for chromosomes 7, 8, 17, 18, X and Y. Control cells demonstrated normal copy number for all chromosomes analyzed. In contrast, Peyronie's disease plaque-derived fibroblasts

demonstrated frequent aneusomies in chromosomes 7, 8, 17, 18 and X and recurrent deletions of chromosome Y. Peyronie's disease nonplaque tunica-derived fibroblasts demonstrated

infrequent chromosomal changes early in culture; however, with repeated passaging the majority of cell cultures demonstrated aneusomies in at least one chromosome. These data indicate that

Peyronie's disease plaque-derived fibroblasts have consistent aneusomies even at early passage and that nonplaque tunica-derived cells from men with Peyronie's disease also

demonstrate chromosomal instability. This suggests that the tunica albuginea of men with Peyronie's disease may be predisposed to undergoing unregulated fibrosis. These findings confirm

the transformed nature of the Peyronie's disease tunical fibroblasts studied in this analysis. While the etiology of these findings is not clear, it is likely that these

pathobiological characteristics contribute to the pathophysiology of this disease process. Access through your institution Buy or subscribe This is a preview of subscription content, access

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Laboratory, VA Medical Center, Hines, USA J P Mulhall, B Nicholson, S Pierpaoli, T Lubrano & T V Shankey * Department of Urology, Loyola University Medical Center, Stritch School of

Medicine, USA B Nicholson, S Pierpaoli, T Lubrano & T V Shankey Authors * J P Mulhall View author publications You can also search for this author inPubMed Google Scholar * B Nicholson

View author publications You can also search for this author inPubMed Google Scholar * S Pierpaoli View author publications You can also search for this author inPubMed Google Scholar * T

Lubrano View author publications You can also search for this author inPubMed Google Scholar * T V Shankey View author publications You can also search for this author inPubMed Google

Scholar CORRESPONDING AUTHOR Correspondence to J P Mulhall. ADDITIONAL INFORMATION This research was funded by a Veterans Administration Career Development Award to Dr Mulhall RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Mulhall, J., Nicholson, B., Pierpaoli, S. _et al._ Chromosomal instability is demonstrated by fibroblasts derived

from the tunica of men with Peyronie's disease. _Int J Impot Res_ 16, 288–293 (2004). https://doi.org/10.1038/sj.ijir.3901170 Download citation * Received: 08 May 2003 * Revised: 08

July 2003 * Accepted: 19 July 2003 * Published: 12 February 2004 * Issue Date: 01 June 2004 * DOI: https://doi.org/10.1038/sj.ijir.3901170 SHARE THIS ARTICLE Anyone you share the following

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SharedIt content-sharing initiative KEYWORDS * Peyronie's disease * chromosomes * flourescent _in situ_ hybridization * fibromatosis