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ABSTRACT AIM: Overexpression of midkine (MK) has been observed in many malignancies. This aim of this study is to screen for suitable antisense oligonucleotides (ASODN) targeting MK in

hepatocellular carcinoma (HCC) cells and evaluate its antitumor activity. METHODS: Ten ASODN targeting MK were designed and synthesized. After transfection with ASODN, cell proliferation was

analyzed with MTS [3 -(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2_H_-tetrazolium, inner salt] assay. In addition, MK mRNA, protein levels, as well as apoptosis

and caspase-3 activity were also examined in HepG2 cells. Cell proliferation was then analyzed after treatment with both ASODN and chemotherapeutic drugs. RESULTS: In this experiment, the

ASODN5 among the 10 ASODN showed higher inhibitory activity against proliferation of hepatocellular carcinoma cells in a dose-dependent manner. In HepG2 cells, ASODN5 could significantly

reduce the MK mRNA level and protein content. After transfection with ASODN5 for 48 h, accompanied with a decline of survivin and Bcl-2 protein content, a remarkable increase of apoptosis

and caspase-3 activity was observed in HepG2 cells. Furthermore, ASODN5 transfer can significantly increase chemosensitivity in HepG2 cells. CONCLUSION: Antisense oligonucleotides targeting

MK shows therapeutic effects on HCC; ASODN5 has the possibility to be developed as an effective antitumor agent. SIMILAR CONTENT BEING VIEWED BY OTHERS DUAL-MODIFIED ANTISENSE

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Huzhou, 313000, China Li-cheng Dai, Xiang Wang & Jian-fang He * Department of General Surgery, Huzhou Central Hospital, Huzhou, 313000, China Xing Yao & Yong-liang Lu * Department of

Pathology, Huzhou Central Hospital, Huzhou, 313000, China Jin-liang Ping Authors * Li-cheng Dai View author publications You can also search for this author inPubMed Google Scholar * Xiang

Wang View author publications You can also search for this author inPubMed Google Scholar * Xing Yao View author publications You can also search for this author inPubMed Google Scholar *

Yong-liang Lu View author publications You can also search for this author inPubMed Google Scholar * Jin-liang Ping View author publications You can also search for this author inPubMed 

Google Scholar * Jian-fang He View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Li-cheng Dai. ADDITIONAL INFORMATION

Project supported by the Zhejiang Province Medicine and Sanitation Research Foundation (2003A077). The Editor has retracted this article because some of the figures have errors which were

introduced during the process of integrating and editing the original images (Figures 3, 4, and 5). Due to the fact that the research was conducted over ten years ago, the original data can

no longer be found, and the article cannot be corrected. All authors agree to this retraction. ABOUT THIS ARTICLE CITE THIS ARTICLE Dai, Lc., Wang, X., Yao, X. _et al._ RETRACTED ARTICLE:

Antisense oligonucleotides targeting midkine induced apoptosis and increased chemosensitivity in hepatocellular carcinoma cells. _Acta Pharmacol Sin_ 27, 1630–1636 (2006).

https://doi.org/10.1111/j.1745-7254.2006.00459.x Download citation * Received: 03 August 2006 * Accepted: 14 August 2006 * Issue Date: 01 December 2006 * DOI:

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not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * antisense oligonucleotide * midkine * apoptosis *

hepatocellular carcinoma * chemosensitivity