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The community-driven initiative Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy

image data through quality control (QC) management of instruments and images. It aims for a common set of QC guidelines for hardware calibration and image acquisition, management and

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JANUARY 2022 A Correction to this paper has been published: https://doi.org/10.1038/s41592-021-01387-x _ REFERENCES * Baker, M. _Nature_ 521, 274–276 (2015). Article  CAS  Google Scholar  *

Bordeaux, J. et al. _Biotechniques_ 48, 197–209 (2010). Article  CAS  Google Scholar  * Eckers, J. C., Swick, A. D. & Kimple, R. J. _Radiat. Res._ 189, 551–552 (2018). Article  CAS 

Google Scholar  * Kniss, D. A. & Summerfield, T. L. _Reprod. Sci._ 21, 1015–1019 (2014). Article  Google Scholar  * Hutson, M. _Science_ 359, 725–726 (2018). Article  Google Scholar  *

Gibney, E. _Nature_ 577, 14 (2020). Article  CAS  Google Scholar  * Clements, J. C. _Nature_ 582, 300 (2020). Article  CAS  Google Scholar  * Franca, T.F. & Monserrat, J.M. _EMBO Rep._

19, e46008 (2018). Article  Google Scholar  * Fanelli, D. _Proc. Natl. Acad. Sci. USA_ 115, 2628–2631 (2018). Article  CAS  Google Scholar  * França, T. F. A. & Monserrat, J. M.

_Bioessays_ 41, e1800206 (2019). Article  Google Scholar  * MacNeil, T. et al. _Biotechniques_ 69, 460–468 (2020). Article  CAS  Google Scholar  * Marx, V. _Nat. Methods_ 11, 483–488 (2014).

Article  CAS  Google Scholar  * Freedman, L. P. et al. _Nat. Methods_ 12, 493–497 (2015). Article  CAS  Google Scholar  * Yu, M. et al. _Nature_ 520, 307–311 (2015). Article  CAS  Google

Scholar  * Ellenberg, J. et al. _Nat. Methods_ 15, 849–854 (2018). Article  CAS  Google Scholar  * Sansone, S. A. et al. _Nat. Biotechnol._ 37, 358–367 (2019). Article  CAS  Google Scholar 

* Nelson, G., Gelman, L., Faklaris, O., Nitschke, R. & Laude, A. Preprint at https://arxiv.org/abs/2011.08713 (2020). * Nelson, G. et al. Preprint at https://arxiv.org/abs/2101.09153

(2021). Download references ACKNOWLEDGEMENTS C.M.B has been funded in part by grant number 2020-225398 from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community

Foundation. The work of C.S.-D.-C. was supported by NIH grant # U01CA200059 and by grant #2019-198155 (5022) by the Chan Zuckerberg Initiative as part of their Imaging Scientist Program.

R.N. was funded by Deutsche Forschungsgemeinschaft (German Research Foundation) grant number Ni 451/9-1-MIAP-Freiburg. We thank somersault18:24 BV (Leuven, Belgium) for help with Fig. 1 and

Thao Do (Allen Institute, Seattle, WA, USA) for the design of the QUAREP-LiMi logo. AUTHOR INFORMATION Author notes * These authors contributed equally: Ulrike Boehm, Glyn Nelson, Claire M.

Brown. AUTHORS AND AFFILIATIONS * Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA Ulrike Boehm * Bioimaging Unit, Newcastle University, Newcastle upon Tyne, UK

Glyn Nelson & Alex Laude * Advanced BioImaging Facility (ABIF), McGill University, Montreal, Quebec, Canada Claire M. Brown * Visualisation, Irradiation & Analysis, Cancer Research

UK Manchester Institute, Alderley Park, Macclesfield, UK Steve Bagley * National Institute of Standards and Technology, Gaithersburg, MD, USA Peter Bajcsy * Euro-BioImaging ERIC, Heidelberg,

Germany Johanna Bischof * Unite Génétique et Biologie du Développement U934, PICT-IBiSA, Institut Curie/Inserm/CNRS/PSL Research University, Paris, France Aurelien Dauphin * Department of

Biochemistry, University of Oxford, Oxford, UK Ian M. Dobbie * Turku Bioscience Centre, Euro-Bioimaging ERIC, Turku, Finland John E. Eriksson * BCM, University of Montpellier, CNRS, INSERM,

Montpellier, France Orestis Faklaris * Centre for Cellular Imaging, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Julia Fernandez-Rodriguez * Imaging Core Facility

Biozentrum, University of Basel, Basel, Switzerland Alexia Ferrand * Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Laurent Gelman * Light Microscopy Facility, CMCB

Technology Platform, TU Dresden, Dresden, Germany Ali Gheisari & Hella Hartmann * FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany

Christian Kukat * Light Microscopy Facility, Max Planck Institute of Experimental Medicine, Goettingen, Germany Miso Mitkovski * VIB BioImaging Core, Leuven, Flanders, Belgium Sebastian

Munck * VIB-KU Leuven Center for Brain and Disease, Leuven, Flanders, Belgium Sebastian Munck * KU Leuven Department for Neuroscience, Leuven, Flanders, Belgium Sebastian Munck * The

Rockefeller University, New York, NY, USA Alison J. North * Scientific Service Group Microscopy, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany Tobias M. Rasse *

Division Biophotonics, Federal Institute for Materials Research and Testing (BAM), Berlin, Germany Ute Resch-Genger * European Molecular Biology Laboratory, Advanced Light Microscopy

Facility, Heidelberg, Germany Lucas C. Schuetz * Faculty of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland Arne Seitz * Program in Molecular Medicine,

University of Massachusetts Medical School, Worcester, MA, USA Caterina Strambio-De-Castillia * Divisions of Computational Biology and Gene Regulation and Expression, School of Life

Sciences, University of Dundee, Dundee, UK Jason R. Swedlow * Life Imaging Center, Albert-Ludwigs University, Freiburg, Germany Roland Nitschke * Life Imaging Center and Signalling Research

Centres CIBSS and BIOSS, University of Freiburg, Freiburg, Germany Roland Nitschke Authors * Ulrike Boehm View author publications You can also search for this author inPubMed Google Scholar

* Glyn Nelson View author publications You can also search for this author inPubMed Google Scholar * Claire M. Brown View author publications You can also search for this author inPubMed 

Google Scholar * Steve Bagley View author publications You can also search for this author inPubMed Google Scholar * Peter Bajcsy View author publications You can also search for this author

inPubMed Google Scholar * Johanna Bischof View author publications You can also search for this author inPubMed Google Scholar * Aurelien Dauphin View author publications You can also

search for this author inPubMed Google Scholar * Ian M. Dobbie View author publications You can also search for this author inPubMed Google Scholar * John E. Eriksson View author

publications You can also search for this author inPubMed Google Scholar * Orestis Faklaris View author publications You can also search for this author inPubMed Google Scholar * Julia

Fernandez-Rodriguez View author publications You can also search for this author inPubMed Google Scholar * Alexia Ferrand View author publications You can also search for this author

inPubMed Google Scholar * Laurent Gelman View author publications You can also search for this author inPubMed Google Scholar * Ali Gheisari View author publications You can also search for

this author inPubMed Google Scholar * Hella Hartmann View author publications You can also search for this author inPubMed Google Scholar * Christian Kukat View author publications You can

also search for this author inPubMed Google Scholar * Alex Laude View author publications You can also search for this author inPubMed Google Scholar * Miso Mitkovski View author

publications You can also search for this author inPubMed Google Scholar * Sebastian Munck View author publications You can also search for this author inPubMed Google Scholar * Alison J.

North View author publications You can also search for this author inPubMed Google Scholar * Tobias M. Rasse View author publications You can also search for this author inPubMed Google

Scholar * Ute Resch-Genger View author publications You can also search for this author inPubMed Google Scholar * Lucas C. Schuetz View author publications You can also search for this

author inPubMed Google Scholar * Arne Seitz View author publications You can also search for this author inPubMed Google Scholar * Caterina Strambio-De-Castillia View author publications You

can also search for this author inPubMed Google Scholar * Jason R. Swedlow View author publications You can also search for this author inPubMed Google Scholar * Roland Nitschke View author

publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS U.B. and G.N. are chair and vice-chair of the QUAREP-LiMi’s White Paper working group (WG8). They led

the effort to write this manuscript and the QUAREP white paper. C.M.B. wrote the first draft of the manuscript based on the QUAREP white paper and coordinated integration of comments and

changes from the authors to realize the final version. All authors contributed to the editing of the manuscript. R.N. coordinates the QUAREP-LiMi initiative. CORRESPONDING AUTHOR

Correspondence to Roland Nitschke. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE

THIS ARTICLE Boehm, U., Nelson, G., Brown, C.M. _et al._ QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy. _Nat Methods_ 18, 1423–1426

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