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ABSTRACT Previously we reported that LPS induced rabbit Møs release protein factors which recruit PMNLs into rabbit skin as measured with 51Cr labelled leukocytes. Here we report that in

vitro stimulation of human monocyte derived Møs with LPS (3-100 ng/ml for 1-24 hours) results in their secretion of at least one protein factor capable of attracting PMNLs into the skin of

rabbits following intradermal injection. The predominant PMNL recruiting activity (PRA) had a molecular weight on gel filtration (Sephadex-100, Superose-12) of 40-45 Kd. The production of

the PRA was inhibited by cycloheximide (2 μg/ml) and the PRA was found to be relatively heat stable (43% loss of activity at 56° C 30 min) and had no in vitro chemotactic activity for PMNLs.

The gel filtration fractions most active for PRA had 7-20 U/ml TNF and no detectable IL-1 (<0.2 U/ml) activity. The active gel filtration fractions were tested after treating them with

neutralizing polyclonal anti-human IL-1α and IL-1β and with neutralizing monoclonal anti-TNFα antibody. This combined treatment decreased the in vivo PRA activity by only 16%. These results

suggest that LPS stimulated human Møs secrete a yet unidentified 40-45 Kd component (PRA) which can be distinguished from TNFα, IL-1 and macrophage derived factors chemotactic for PMNLs in

vitro. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Szent-Györgyi Albert Medical School, Szeged, Hungary Pál J Megyeri & Andrew C Issekutz * Dept of Pediatrics and

Microbiology Dalhousie Univ, Halifax, N.S., Canada Pál J Megyeri & Andrew C Issekutz Authors * Pál J Megyeri View author publications You can also search for this author inPubMed Google

Scholar * Andrew C Issekutz View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE Megyeri, P., Issekutz, A. STUDIES OF ENDOTOXIN (LPS) INDUCED HUMAN MACROPHAGE (MØ) PRODUCTS WHICH INDUCE POLY- MORPHONUCLEAR LEUKOCYTE (PMNL) RECRUITMENT DURING INFLAMMATION.

_Pediatr Res_ 26, 510 (1989). https://doi.org/10.1203/00006450-198911000-00064 Download citation * Issue Date: 01 November 1989 * DOI: https://doi.org/10.1203/00006450-198911000-00064 SHARE

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